Purpose：Symptoms of visual fatigue are associated with prolonged near work. This study investigates the effects of visual fatigue on accommodation system, to find a correlation between visual fatigue and the use eye. We perform clinical measures of binocular functions and accommodation function. Methods：49 visually normal, young-adult subjects without presbyopia and eye diseases participated in the study. Ocular examination included visual acuity, autorefraction, subjective refraction for best corrected vision, Von Graefe test for phoria, test in near point of convergence, near point of accommodation, minus and plus lens to blur, fused crossed cylinder, negative relative accommodation, positive relative accommodative and accommodation facility. A survey of visual discomfort developed by Conlon et al. (1999), were administered to subjects before the clinical assessments. Results：32.7％ of the subjects have asthenopia, While 67.3％ of the subjects do not. Comparing to the ones with no visual fatigue, Subjects that with asthenopia have significant difference in accommodative amplitude(t＝-4.575，p＜0.05) and accommodative facility t＝4.016，p＞0.05). Statistically significant correlation was noted between visual fatigue and near work time(r＝0.311, p＝0.029＜0.05), computer usage time(r＝0.312, p＝0.029＜0.05), Smartphone usage time(r＝0.384, p＝0.006＜0.05), TV watching time(r＝0.283, p＝0.049＜0.05), and visual discomfort frequency(r＝0.656, p＝0.000＜0.05). Statistically significant risk for asthenopia included NPC break point(OR＝3.850, CI＝1.078~13.751), NPA(OR＝3.833, CI＝1.093~13.450), accommodative amplitude(OR＝1.941, CI＝1.394~2.703), and accommodative facility(OR＝5.60, CI＝1.429~21.949). Discussion：Our results show that visual fatigue is associated with near work time, 3C electronic products usage, frequency asthenopia. The accommodative system exhibited fatigue with decreasing accommodation amplitude and accommodative facility. Near visual functions are affected by asthenopia. It is essential for government to mandate public health policy for in the protection eye health general public is necessary.