Protein kinases play important roles in the regulation of several brain-specific functions, including neuronal differential, neuronal plasticity, LTP (long-term potentiation)，LTD (long-term depression) and neurotransmitter release. In the previously studies, we have identified a novel serine/threonine protein kinase, BSK146, in the brain of zebrafish. In the present data suggest that BSK146 may involve in hindbrain development. By using the BLAST program to search the currently available database, we found a BSK146 homologue, rat SH3-binding kinase (sbk), with distinct kinase domain and proline-rich C-terminal region. To identify this novel protein kinase function, we isolated and characterized a mice protein kinase msbk, which is homologous to that of rat SH3-binding kinase (sbk), and explore this novel protein kinase possible mechanism. Using pull-down approach, we have identified a candidate msbk interaction protein in astrocytes cell lysate, MYH9 (non-muscle myosin heavy chain IIA). In addition to elucidate the regulation of msbk gene expression, we analyzed the 5kb fragment corresponding to the 5’-upstream region of msbk gene, revealed numerous putative binding sites for transcription factors. Promoter analysis proved that the mSBK 7th fragment had promoter activity in nerve cells. By using the immuno-fluorescence analysis also found the MYH9 in nerve cells. In this study, we have identified a candidate interaction molecule for msbk and revealed numerous putative regulation elements. Whether the mSBK were associated with MYH9 to affect the downstream protein and regulated the other specific brain functions or nerve cells to migrate properly during developmental stages need to be further explored.