The aim of this study was to investigate stigmatization by the guards in the correctional system for the prisoners with HIV, and to understand the influence of the stigma. The study data was collected by face-to-face in depth interviews, and the participants were 8 male prison guards. The interviews were audio recorded and translated verbatim. This study used grounded theory as the research method. The content analysis showed that the guards who have contact with prisoners with HIV were more friendly, those who never or seldom had contact with prisoners with HIV thought that it was more difficult to manage those prisoners with HIV, the stress was heavy, and the environment was dangerous; they were afraid of being infected by the prisoners’ blood. That was the impact of stigma. Other than this, the guards have negative stereotypes about the prisoners with HIV. Separating those prisoners with HIV from others caused their HIV status to be exposed. The study also found that increasing knowledge was one of the key tools to reduce stigma. That is, the guards had correct knowledge about HIV/AIDS, then stigmatization would be decreased. The interviewees also pointed that if education should be reinforced, the guards would be more willing to guard the HIV/AIDS wards. According to the results, the study suggests increasing the interaction between guards and prisoners with HIV, and increasing counseling courses. The researcher reflects that the study would be more complete if it contained the interviews with prisoners. Further, it is also important to use the adaptive method of interviewing in the future in order to lower the reaction of interviewees to answer in the direction of social desirability.