The glass is ectensively applied in the industry and the daily necessities, for examply biomedical engineering, microelectromechanical, sensor, copper oxide photovoltaic cell, touch screens, glasses, and allkinds of windows, etc…many of these glass used in public places, and in the public places a lot of dirt and glass residue virus. If we can use a simple coating on glass reduces the adhesion of dirt and bacteria breeding, will be able to increase the aesthetics of each other and to avoid contagious diseases to environmental pollution.To promote the improvement of the value of gless, the society will be a great boon. Some self-assembled monolayer film made simple, In the past, already has a stain-resistant and reliable and stable system, but mixed self-assembled film fow success stories in the research, because of factors made complicated and not easy to control OTS mixed self-assembled film made simple step, low cost these thesis used taguchi method design and find the best of made arguments, so we can simply be coated on the glass and increasing antibacterial function.Let this coating multifunctional.The experimental results show Ethanol, Toluene, n-Heptane, compared with eachother in different solvents, tound effect, in the soaking temperature 30 ℃, ding temperature 25 ℃,soaking time 6 hrs, as hoc agent concentration 15 mM , under condition of Antibacterial agent concentration 10% contactis reach 130.1°. Surface adhesion reduced original 80%, and this film antimicrobial effects made bacteria reach only 0.63 CFU / cm2, much higfer than the industry and lifestyle applications, standard. The second part of the use sol gel method to as the precursors, different solvents, different additives etc, paremeeters of the glass surface modification, and plated OTS mixed self-assembled film contact angle of up to 117° expressed with stain resistance and antibacterial.