Objective: Handoffs and blood transfusion reaction management, which are critical to patient safety and care quality, are essential competence in nursing practice. This study analyzes the effectiveness of the new nurse training program with simulation teaching on clinical practice, team communication and nursing handoffs. Methods: A total of 60 new nurses were included in the study: those employed from June to July of 2016 as the experimental group (N=30) with simulation teaching and those employed from August to September of 2016 as the control group (N=30) receiving conventional education. Independent and paired t-tests were used to compare knowledge, skills and confidence between the two groups and before and after intervention. Results: After 2-week intervention, the mean score on knowledge in the experimental group (92.6) was higher than in the control group (82.0) (P＜.001); the experimental group showed a higher confidence score in handoff skills and blood transfusion reaction than in the control group after a 4-week follow-up. There was a difference between pretest and posttest mean scores in the experimental group, with 86.1 vs 92.6 for knowledge (P＜.001) and 9.8 vs 12.4 for confidence (P＜.001), respectively. Conclusion: The study results confirm that the new nurse training program that based on the OSCE evaluation with simulation teaching can improve the cognition of handoff communication and blood transfusion reaction management in new nurses and strengthen their confidence in clinical practice.