Fluid supplement, total parenteral nutrition, and medication necessitate an intravenous access for an extended period of time. Compared to peripheral intravenous accesses, PCVCs are better tolerated for hypertonic fluids and stimulating medications. In addition, the patients experience less puncture frequency than with peripheral intravenous accesses with an indwelling time of 3 days only. The complications of PCVC include catheter-related bloodstream infection (CRBSI), broken catheter, phlebitis, and occlusion. CRBSI may lead to sepsis and prolonged hospitalization. This study was conducted from March to October 2019. The incidence of the complications of PCVCs before and after the intervention was compared. The intervention was to clean the skin, followed by applying povidone iodine on the skin for at least 2 minutes, and to use maximum sterile barrier precautions during weekly dressings on the PCVC injection site. It was found that the incidence of CRBSI significantly decreased after the intervention during dressing change at the PCVC injection site, from 2.7/1,000 catheter-day to 0/1,000 catheter-day, which shortened the hospitalization days, reduced healthcare cost, and improved the quality of neonatal care in the NICU.