Cancer patients frequently rely on an implanted infusion catheter (Port-A) for injection of anti-cancer drugs; however, Port-A-related bloodstream infections affect patient outcomes and increase mortality. Thus, to maintain good quality of care and patient safety, we utilize cooperative interdepartmental teamwork and medical breakthrough series techniques (BTS) to formulate improvement strategies including: (1) introduction of 2% chlorhexidine gluconate for skin disinfection; (2) a training program and production of a Port-A health education video combined with action task vehicles; (3) introduction and execution of a maximal protection policy grade; (4) implementation of a hand hygiene compliance and accuracy audit; (5) development of specialist quality control indicators and clinical outcome indicators according to the Taiwan Clinical Practice Improvement (TCPI) indicators used to define oncology ward ＂Port-A-related bloodstream infections＂; and (6) establishment of Port-A teaching model aids. The implementation of the above strategies resulted in a decrease in Port-A-related bloodstream infections from 2.01% to 1.50% (decreased 0.51%), decrease in oncology ward healthcare-associated bloodstream infections from 1.65% to 1.24% (decreased 0.41%), increase in the accuracy of the Port-A infusion procedure from 58.8% to 100% (increased 41.2%), and decrease in the average oncology ward hospital stay from 8.2 to 7.1 days (shortened by 1.1 days). Cooperative interdepartmental teamwork, establishment of standard Port-A infusion procedures, and implementation of hand hygiene audits and skin disinfection techniques can reduce Port-A-related bloodstream infections.