Obesity is a critical issue worldwide. According to Nutrition and Health Survey in 2013 indicated that prevalence of overweight and obesity was 38% in Taiwan people. And World Health Organization (WHO) indicate that compare to the normal weight people the obesity people have higher risk of chronic disease which include diabetes mellitus, dyslipidemia, hepatic steatosis and hypertension. The concept of drinking tea is for a long time and widely accepted. However, the strain of Andraca theae which lives on tea leaf and its metabolites called the tea metabolites from Andraca theae that bioactivity is unknown yet. Consequently, this study was aimed to investigate whether extract of TTES No.12 and its metabolites from Andraca theae (TME) have the same or better effect than TTES No.12 tea extract (TE) on prevention of obesity and obesity-induced chronical disease. The animal model used high fat diet and different dose of TME and TE for a period of time. After administrated TME improved serum total cholesterol parameters. However, after administrated TE, our results showed that TE significantly decreased all adipose tissue and adipocyte size via increasing Pref-1and inhibiting downstream signaling pathway. However, TE also significantly improved fatty hepatocytes via increasing Ho-1. Taken together, these results suggested that the content of tea polyphenols play an important role for the prevention of obesity and fatty liver disease.