Organic agriculture has been higher and higher valued in recent years due to environmental protection concern. However, the research on the production of organic F1 hybrid seed is insufficient. This research focused on organic cultulral management and organic seed production of Chinese cabbage (Brassica rapa L. Group Pekinensis) ʻLucky Star No.2.ʼ After cover by black rot pathogen (Xanthomonas campestris pv. campestris), Chinese cabbage seeds were treated with 60℃ or 70℃ dry heat for 24, 36, or 48 h. Seeds treated by 70℃ dry heat for 36 h exhibited the highest sterile effect 98.3% without affecting seed germination. Organic cultural substrate was added with 1~3% organic vegetable fertilizer, 5~10% vermiculite or 5~10% carbonated rice hull and used as seedling substate. The substrate with 3% organic vegetable fertilizer, 5% vermiculite and 5% carbonated rice hull significantly showed the highest shoot dry weight 54.6 mg at the 3rd week in nursery stage. Three kinds of repellent plants, peppermint, rosemary and sage, were planted aside or around Chinese cabbage plants with distance of 10, 30 or 60 cm. The lowest pest index 54.1% was exhibited in the treatment of planting peppermint aside with distance of 30 cm which is easy to cultivate. Lacewings and ladybugs were released at the 0th, 1st or 3rd week after transplanting, once per week, twice per week or once per 2 weeks. Releasing lacewings once per week started from the 1st week after transplanting showed the highest suppression effect with aphid pest index 46.7% which is easy to conduct. Trichoderma spp. or phosphate-solubilizing bacteria of 10, 20, or 40 mL˙L-1 was sprayed on the seedlings after transplanting once per 5, 10, or 15 days. Spraying 20 mL˙L-1 Trichoderma spp. once per 10 days could keep the damping-off away from the seedlings without affecting plant growth. Spraying 40 mL˙L-1 phosphate-solubilizing bacteria once per 15 days over seeds showed the most prominent growth-promoting effect with shoot fresh weight 73.14 g. Seedlings of inbred parents of ‘Lucky star No.2’ were incubated at 5-7℃ with light/dark period 23/1, 16/8 or 12/12 h for 10, 15 or 20 days, respectively. Inbred parents vernalized by 5-7℃ for 20 days with light/dark period 23/1 h produced the most flowering branches of 59, which was the best vernalization condition.