Nanotechnology is the science of the very small and involves the manipulation of matter at the atomic or molecular level. Nanoparticles possess high surface-to-volume ratio and quantum effects and are broadly employed in the developments of electronics, renewable energy and medication. However, latest research has demonstrated that nanoparticles may exhibit cytotoxicity. Because of its small size, nanoparticles can interact with the cell membrane resulting in perforation or death of cells. As a consequence, the study of the interaction between nanoparticle and cell membrane is of great importance. In this work, the dissipative particle dynamics is employed to investigate the mechanism of nanoparticle-supported lipid bilayer (SLB) interaction. SLB is an ideal model for cell membrane since it is more stable than a freely suspended membrane. It is found that lipids tend to adsorb onto nanoparticles as temperatures increases and the adsorption curves exhibit two peaks at the pre-transition temperature and the main transition temperature. Furthermore, our results show that the hydrophobicity of a nanoparticle needs to exceed a critical value before the nanoparticle-SLB interaction takes place and the critical hydrophobicity varies with temperature. We also find that the adsorption area density of lipids on small-sized nanoparticle is greater than that of large-sized counterpart. However, as temperature increases, large-sized nanoparticles have the ability to perforate the lipid bilayer. Johnson-Mehl-Avrami-Kolmogorov equation is used to correlate the variation of the perforation surface area with time. The results reveal that the perforation mechanism of the membrane is essentially the same as the one dimension, heterogeneous nucleation process and is independent of the size, hydrophobicity and number of nanoparticles in the system. This work can be applicable to prevent harmful effects of nanoparticles to our body. It can also be used to predict the reaction rate and reaction temperature to maximize the cytotoxicity of the nanoparticles when it is applied as an anticancer drug or disinfectant.