Chilling injury of postharvest fruit in bitter gourd 'Moon Shine' was investigated. The ethylene production and respiratory rate increments of mature green fruits was positively correlated to temperature increments from 2 to 25 C. The lag phase of the preclimacteric period of the fruit was shortened in higher storage temperature. Symptoms of chilling injury in fruit included surface pitting, loss of yellowing and softening ability, water-soaking and breakdown. Those symptoms were developed in harvested fruits stored at 2°C for more than 2 days, and at 8°C for more than 7 days, respectively.