Objectives: To explore the impact of different ＂color contrast＂ on the computer screen for low vision readers. Methods: This study focused on patients over 20 years old, the best corrected-eyesight is between 0.05 (included) and 0.3, able to use a computer, and without a reading disorder or blindness in a general ward of a Taipei medical center from February 3, 2016 to December 31, 2017; there were 60 valid samples. 300 words were selected randomly constituted four essays without specific subjects and with Font 18 PMing Liu characters, and displayed in different color contrast (text/background) as white/black, black/white, white/green, and yellow/blue on the monitor. After patients with low vision finished reading the screen, we then collected and recorded their reading speed, error rate, and made them complete a questionnaire to find out their preferences and ideas about all the four essays. After data coding, ANOVA test was performed with SPSS for Windows 18.0 with Percentage and Mean. Results: Reading time: Yellow / blue is significantly higher than the other three groups. Error rate: white/black superior than the others. The white/black has the best word recognition and is more comfortable to read. Generally, white/black gets the highest satisfaction for the readers with low vision. Conclusions: The computer screen designed with ＂black background and white words＂ enables low vision people to have clearer visibility, and not only enhances reading speed, but also reduces reading error rate, and fatigue as well.