Qigong is a health cultivation method derived from Taoist in ancient China, and as a form of complementary and alternative medical therapy in the United States. The skills of qigong exercise emphasize the "relaxation" of the body, the "quietness" of the mind and the "naturalness" of movements. It has the effect of regulating negative emotion and keeping the body and mind in a harmonious state, through deeply breathing and concentration. In recent years, increasing number of studies have documented the effectiveness of qigong exercise in helping people improve their physical health and reduce perceived stress, anxiety and depression. Therefore, the purpose of this article is to summarize the researches to understand the regulatory effects and mechanisms of applying qigong exercise in negative emotions. Results show that qigong exercise reduce the symptoms of anxiety and depression in adults, adolescents, elderly and people with chronic disease, which illustrated qigong can regulate the emotion of anxiety and depression. In conclusion, this article provides the explain of regulatory mechanisms for qigong regulates negative emotion from the perspectives of psychological, physiological and neuroendocrine to demonstrate qigong exercise balance human body from a multiplicity of directions.