Additional file 5: of Giardia intestinalis mitosomes undergo synchronized fission but not fusion and are constitutively associated with the endoplasmic reticulum

Peripheral mitosomes divide during all stages of mitosis. (A) G. intestinalis culture was enriched for mitotic trophozoites by albendazole treatment (100 ng/mL) for 6 h at 37 °C. The cells were washed twice in warm medium and fixed, and the mitosomes were immunolabeled with an anti-GL50803_9296 antibody (red) and stained for nuclei with DAPI (blue). The image represents a deconvolved maximal projection of the Z-stack. Corresponding DIC images are shown. Scale bar, 2 μm. Arrowheads point at dividing mitosomes. (B) The number of mitosomes in particular stages of mitosis was determined using fixed cells. The data show a gradual increase in mitosome number during mitosis. Thirty cells of each mitotic stage were used for the statistics. The error bars represent the standard deviations. (EPS 4730 kb)