Additional file 3: Figure S2. of A novel set of vectors for Fur-controlled protein expression under iron deprivation in Escherichia coli

Nucleotide sequence of gBlock2 containing kanamycin resistance gene. Black text: Kanamycin resistance gene from pKT25. Red text: Sequence overlapping with pFCF1 region upstream of NcoI restriction endonuclease site. Green text: Sequence overlapping with pFCF1 region downstream of ScaI restriction endonuclease site. Orange text: Region encoding the HA tag. Start codon is highlighted in yellow. (TIF 320 kb)