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Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence-4
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posted on 2011-12-31, 15:25 authored by Anke Batsch, Andrea Noetel, Christian Fork, Anita Urban, Daliborka Lazic, Tina Lucas, Julia Pietsch, Andreas Lazar, Edgar Schömig, Dirk GründemannRbitrarily chosen; numbering refers to the Excel raw data file [see Additional file ]) was analyzed. After subtraction of linear background, EA was calculated as F/Fratio and plotted against fluorescence (F) as shown. To avoid confusion, points with F< 0.1 are not displayed; most of these, because of large errors in EA, lie outside the y axis range. The line graphs were drawn with the Gauss peak function (upper row) or the logistic peak function (lower row). Note that function parameters were not fitted to the points shown, but determined by our stepwise PCR simulation approach based on the raw fluorescence versus cycle number data. Open circles represent data that was not used for fitting.
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Taken from "Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence"
http://www.biomedcentral.com/1471-2105/9/95
BMC Bioinformatics 2008;9():95-95.
Published online 12 Feb 2008
PMCID:PMC2276494.
