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Verification of Amplicon Specificity using Restriction Enzymes (VASRE) on two real-time PCR amplicons
figure
posted on 2011-12-31, 01:43 authored by James J. Yun, Lawrence E. Heisler, Irene I. L. Hwang, Olivia Wilkins, Suzanne K. Lau, Martin Hyrcza, Bamini Jayabalasingham, Jing Jin, JoAnne McLaurin, Ming-Sound Tsao, Sandy D. DerCopyright information:
Taken from "Genomic DNA functions as a universal external standard in quantitative real-time PCR"
Nucleic Acids Research 2006;34(12):e85-e85.
Published online 13 Jul 2006
PMCID:PMC1524913.
© 2006 The Author(s)
Expected digestion patterns are shown in the panels on the right. Dissociation curves after digestion with HpyCH4V and RsaI confirm the specificity of two amplicons when compared to expected results.