12967_2023_3935_MOESM1_ESM.ppt (713 kB)
Additional file 1 of Short term starvation potentiates the efficacy of chemotherapy in triple negative breast cancer via metabolic reprogramming
presentation
posted on 2023-04-13, 15:07 authored by Ioannis S. Pateras, Chloe Williams, Despoina D. Gianniou, Aggelos T. Margetis, Margaritis Avgeris, Pantelis Rousakis, Aigli-Ioanna Legaki, Peter Mirtschink, Wei Zhang, Konstantina Panoutsopoulou, Anastasios D. Delis, Stamatis N. Pagakis, Wei Tang, Stefan Ambs, Ulrika Warpman Berglund, Thomas Helleday, Anastasia Varvarigou, Antonios Chatzigeorgiou, Anders Nordström, Ourania E. Tsitsilonis, Ioannis P. Trougakos, Jonathan D. Gilthorpe, Teresa FrisanAdditional file 1: Fig. S1. Starvation preferentially sensitizes different breast cancer subtypes versus near normal cells to chemotherapy. a. In vitro treatment overview: cells were glucose/serum starved (STS) for 48 h (blue bar), drugs were added during the last 24h (red bar). b. MTT survival assays after 48 h treatment with STS with or without addition of doxorubicin (DXR) in near normal MCF-10A cells and the indicated breast cancer cell lines. Data are presented as mean survival percentage. *P ≤ 0.05. c. MTT survival assay after 48 h treatment with STS with or without 100 nM doxorubicin (DXR) in near normal MCF10A cells and the triple negative breast cancer cells MDA-MB-231, MDA-MB-468 and HS578. Data are presented as mean survival percentage. *P ≤ 0.05. d. MTT survival assays after 48 h treatment with STS with or without addition of cisplatin (CIS) in near normal MCF-10A cells and the indicated breast cancer cell lines. Data are presented as mean survival percentage. *P ≤ 0.05.
