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Additional file 4 of Transcriptome analysis of a newly established mouse model of Toxoplasma gondii pneumonia

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posted on 2023-04-13, 13:06 authored by Long Cheng, Sajid Ur Rahman, Hai-Yan Gong, Rong-Sheng Mi, Yan Huang, Yan Zhang, Ju-Liang Qin, Cheng-Cong Yin, Min Qian, Zhao-Guo Chen

Additional file 4: Figure S1. Verification of transcriptome accuracy by qRT–PCR. The x-axis represents the DETs, and the y-axis represents the relative expression of the gene. DETs above the horizontal line are upregulated and those below the horizontal line are downregulated. qRT–PCR: quantitative real-time PCR; DETs: differentially expressed transcripts.

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Shanghai Agriculture Applied Technology Development Program Shanghai Science and Technology Commission Scientific Research Project National Key Research and Development Program of China Major Scientific Research Tasks of the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences Central Public-interest Scientific Institution Basal Research Fund National Data Center of Animal Health

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qrt – pcr differentially expressed transcripts lung tissue sections lung pathology examination lung morphological observation transcript enrichment analysis evans blue staining clinical cases due 5 days post identified 3167 dets host immune defenses transcriptome analysis helped kegg enrichment analyses 6 mice infected results provided dets new mouse model reported toxoplasma pneumonia good disease model gondii lung infection toxoplasma gondii infection transcriptome analysis mice infected lung tissues mouse model toxoplasma gondii toxoplasma pneumonia clinical manifestations 5 dpi dets showed dets ).1880 dets innate immune immune system nasal infection gondii rh typical symptoms severe pneumonia ruffled fur rh strains results c57bl respiratory crackles quantitative real pulmonary edema performed using pathological damage molecular mechanisms microbial infections mediated signaling kyoto encyclopedia inflammatory signaling graphical abstract gene ontology e staining dpi ).control group buffered saline biological relevance better understand

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