Additional file 1 of Oxidized LDL promotes EMS-induced angiogenesis by increasing VEGF-A expression and secretion by endometrial cells

Additional file 1: Figure S1. OxLDL upregulated VEGF-A expression and secretion via OLR-1/PI3K/Akt signalling. (A&B) p-AKT and HIF-1α expression in each group of hESCs treated with oxLDL (50 μg/ml) or PBS for 48 h was examined by Western blots. The histogram represents the greyscale of each lane from the Western blot. The number in each group was 3. * represents p < 0.05. (C&D) p-AKT and HIF-1α expression in the hESCs treated with oxLDL (50 μg/ml) or PBS for 48 h was examined by immunofluorescence. (E&F) VEGF-A expression in each group of hESCs treated with oxLDL (50 μg/ml) for 48 h, the AKT inhibitor perifosine (20 mM) and HIF-1α LW6 (20 mM) was examined by Western blotting. The histogram represents the greyscale of each lane from the Western blot. The number in each group was 3. * represents p < 0.05.