We profiled two HCC tumor tissues (HCC01 and HCC03) using a new DNA nanoball (DNB)-based spatial transcriptomics technology named Stereo-seq, and segmented the data into 100 * 100 DNB bins (bin 100 resolution, ~50μm diameter). After basic quality control and filtering, we retrieved transcriptomic information from 36,776 and 23,941 bins from HCC01 and HCC03 tumors respectively, with ~4,000 genes and ~14,500 unique molecular identifiers (UMIs) per bin.