Additional file 9 of Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Additional file 9: Fig. S9. 8p11.22 amplification is associated with PDAC metastasis, related to Fig. 5. A Western blot assays were conducted for detecting ADAM9 protein in PANC-1 cells after si-ADAM9-1 and si-ADMA9-2 knocked down. B The scatter plot depicting the correlation between ADAM9 expression and expression of ADAM9-interacting proteins (Spearman’s correlation). C The scatter plot depicting the correlation between the abundance of SRC and pathways (GSVA score). D The boxplots indicating the protein expression of ADAM9 (left) and CDCP1 (right) between tumor tissues and NATs in CPTAC cohort (Student’s t test). E Spearman-rank correlation of ADAM9 and CDCP1 protein expression (left). The scatter plot on the right depicting the correlation of CDCP1 and SRC protein expression in CPTAC cohort (Spearman’s correlation). F Spearman-rank correlation of the abundance of ADAM9 and epithelial cell migration (GSVA score) in CPTAC cohort (Spearman’s correlation). G Heatmap of the relative abundance of epithelial-cell-migration-related proteins that are significantly associated with ADAM9 expression in CPTAC cohort. H WGCNA of 93 PDAC samples depicting module eigengenes (MEs) highly correlated with primary tumor in the patients with five metastasis sites (upper heatmap of the left panel). Enrichment analysis for the different MEs is presented in the lower heatmap of the left panel (P value < 0.05). The heatmap (right) shows the differential proteins involved the overrepresented pathways of different modules corresponding to the five metastasis sites. I Protein-regulatory network showing proteins participated in metastasis-sites-associated pathways. J Boxplot of ADAM9 protein expression among five metastasis sites (Kruskal-Wallis test). **** p < 1.0E-4, *** p < 1.0E-3, ** p < 1.0E-2, * p < 0.05, ns > 0.05.