Additional file 4 of Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury

Additional file 4: Figure S4. Neuronal expression of osteoprotegerin is upregulated by nuclear calcium signaling and neuronal activity in TBI. A) Negative Control, baseline OPG (TNFRSF11B) signal and a positive Control of the in situ hybridization 24h post injury. B) OPG (TNFRSF11B) in situ signal is mainly found in neuronal sources (neuronal vs non-neuronal 75.38 ± 1.83% vs 24.62% ± 1.83%). N=5. C)-D) Significant increase of OPG (TNFRSF11b) mRNA density 24h post-TBI compared to sham (CS vs CT; 8.53 ± 2.45 vs 28.74 ± 12.03). Buffering of nuclear calcium in TBI significantly decreased OPG mRNA density (CT vs PT; 28.74 ± 12.03 vs 12.14 ± 1.58). Small datapoints depict individual neurons, red datapoints depict average per animal mean ± SD. N=4 (used for statistics). Scale bar: 20µm. E)-F) Buffering of nuclear calcium in TBI significantly decreased OPG mRNA density (CT vs PT; 13.46 ± 5.41 vs 6.03 ± 1.75).mall datapoints depict individual neurons, red datapoints depict average per animal mean ± SD. N=4 (used for statistics). *: p < 0.05; **: p < 0.01. Scale bar: 20µm.