Additional file 1 of The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Additional file 1: Fig. S1. Generation of TET Gene Knockout hESC Lines, Related to Fig. 1. (A) Design of the CRISPR targets for TET genes, using gRNAs (red arrows) that target the sequences corresponding to the beginning of the catalytic domain in TET1, TET2 and TET3; (B) The efficiency for the homozygous knockouts of the TET alleles. The knockouts (KO) were confirmed as bi-allelic frame-shift nonsense mutations. The others include wild-types or heterozygous mutants, or alleles with deletions/insertions of 3 × N base pairs; (C) The DNA sequences of both alleles for the indicated knockout lines. Red letters indicate the positions of the guide RNAs. del: deletion; ins: insertion; (D) Sanger sequencing of TKO hESCs in TET gene target locus.