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Additional file 5 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway
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posted on 2022-10-06, 07:36 authored by Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long, Guoping ZhuAdditional file 5: Fig. S5. The Wnt/β-Catenin pathway was affected by hypoxia. (A) The expression of canonical Wnts (Wnt3 and Wnt8a) and (B) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in AB2.2 mESCs undergoing differentiation. (C) The expression of canonical Wnts (Wnt3 and Wnt8a) and (D) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in R1 mESCs undergoing differentiation. (E-G) Expression of nuclear β-Catenin was significantly repressed, while that of cytoplasmic β-Catenin was upregulated until day 3 and downregulated on day 4 with hypoxia treatment. *, significant (P<0.05).
