Visualization 8

E. coli cell bodies attached to a thin layer of agar substrate, with fluorescently labeled filaments moving fluid tracking particles. Pseudo-colored synchronized representations of the combined, phase contrast and fluorescence sequences computed from movie Visualization 7. Upper left: pseudo-colored version of sequence Visualization 7, with phase contrast pixels (field 1) colored in red and fluorescence pixels (field 2), colored in green. Upper right: de-interlaced/interpolated superimposed phase contrast (red – cell body and tracking particles) and fluorescence (green – filaments) sequences. Lower left: de-interlaced/interpolated fluorescence-only sequence (filaments). Lower right: de-interlaced/interpolated phase contrast-only sequence (cell body and tracking particles). For proper viewing, please download the video file and play it from your computer using software such as ImageJ.