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Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008.pdf (7.32 MB)

Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008

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posted on 2015-09-23, 20:03 authored by Prof. Hesham N. MustafaProf. Hesham N. Mustafa, Kariman Mohammed El-Gohari, Hassan Mostafa Serry, Shahira Samir Zaki, Ezz El-Deen Helmy Helaeil

Acrylamide has several effects toxic and carcinogenic. The current project aimed at exploring the harmful effects of acrylamide on the structure of the stomach, cerebellum and testis in the albino rat, in an attempt to clarify its potential risk on the human health.

The stomach, testis and cerebellum specimens were collected form fifty adult male albino rats. The animals were divided into two main groups. Group (I) was subdivided into 3 subgroups, control, Ia that received acrylamide in a dose of 25mg/kg/10days (P.O.) and Ib that received acrylamide in a dose of 25mg/kg/10days (I.P.). Group (II) was subdivided into 3 subgroups, control, IIa that received acrylamide in a dose of 50mg/kg/10days (P.O.) and IIb that received acrylamide in a dose of 50mg/kg/10days (I.P.).

At the end of the experiment, all rats were anesthetized using ether inhalation. Specimens from testis, cerebellum and stomach were extracted and processed for light and electron microscopic examination. For the light microscope serial sections 5-6µm thick obtained, stained with H&E (Testis, Cerebellum and Stomach); Feulgen stain (Testis) and silver stain (Modified Glees) (Cerebellum). Testicular specimens were studied with the Image analysis technique so as to assess cellular apoptosis. Moreover, DNA cytometry of the basal compartment of the testis was performed.

In the present work, it was found that the testis of the group treated with a dose of 25mg/kg/10days showed mild affection whether acrylamide was administered orally or intraperitoneally. On the other hand, the testis of the group treated with a dose of 50mg/kg/10days showed damage especially with the intraperitoneal administration in comparison to the oral treatment. This was in the form of degeneration of germ cells, numerous multinucleated giant cells with sloughed seminiferous epithelium, and vacuolations in-between the germ cells.

In addition, feulgen stain was performed to assess the effect of acrylamide on cellular apoptosis in the testis. It was clearly proved that acrylamide at a dose of 50mg/kg/10days showed increased apoptosis.

Image analysis of the feulgen stained sections was performed to evaluate the carcinogenicity of acrylamide on testis. It was found that acrylamide at a dose of 50mg/kg/10days showed increased abnormal DNA content in cells, which was considered a reliable marker for malignancy.

As regards effect of acrylamide on the cerebellum, it was concluded that the Purkinje cells were target cells to the acrylamide. Rats treated at a dose of 25mg/kg/10days showed that Purkinje cells somata appeared unaffected, while degeneration of Purkinje cells dendrites and corresponding axons were evident in the molecular and white matter respectively. Furthermore, rats treated with 50mg/kg/10days showed argyrophilic Purkinje cells somata and their dendrites were clearly evident in the Purkinje cell and molecular layers respectively. Also, Purkinje cell axon degeneration was observed in the white matter in the form of fragmented, linearly arrayed debris. The intraperitoneal treatment showed more damage as compared to the oral one.

The stomach of animals treated with acrylamide in a dose of 25mg/kg/10days showed no gastric affection, while there were mild degenerative changes and an apparent increase in mucous secreting cells in the group that received 50mg/kg/10days.

The present study pointed out the hazards of acrylamide and its possible effect on human health. Recommendations are necessary to decrease acrylamide level in different foods and ways to decrease acrylamide formation during preparation of the different foods should be advertised.

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