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Paralogs in the PKA regulon traveled different evolutionary routes to divergent expression in budding yeast.
Notes on scripts used to generate each figure are in:
PKA_evolution_fig_notes.txt
Plasmids are shared at addgene.
Code repositories and their corresponding data is as follows:
https://github.com/heineike02/yeast_esr_expression
expression_data
https://github.com/heineike02/y1000plus_tools
y1000plus_tools_data
https://github.com/heineike02/image_analysis
microscopy
https://github.com/heineike02/PlateReaderTools
plate_reader
RNA-seq data is also on GEO at: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE163741
and code for mapping raw reads is at:
https://github.com/heineike02/rna_seq_processing
https://github.com/heineike02/UTR_annotation
Funding
Unraveling the quantitative dynamics of cAMP-PKA signaling in yeast
National Institute of General Medical Sciences
Find out more...Post 9/11 GI Bill
National Defense Science and Engineering Grant
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