High-Throughput Analysis of Foodborne Bacterial Genomic DNA Using Agilent 2200 TapeStation and Genomic DNA ScreenTape System

The initial step in Next Generation Sequencing is to construct a library from and genomic DNA. To gain the optimum result, extracted DNA must be of high molecular weight with limited degradation. High-throughput sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of genomic DNA extracts. In this study, assessment of the applicability of the TapeStation was done using genomic DNA from nine foodborne pathogens using several accepted high-throughput methods. The Agilent 2200 TapeStation System with Genomic DNA ScreenTape and Genomic DNA Reagents
was easy to use with minimal manual intervention. An important advantage of the
2200 TapeStation over other high-throughput methods was that high molecular
weight genomic DNA quality and quantity can be quantified apart from lower molec-
ular weight size ranges, providing a distinct advantage in the library construction
pipeline and over other methods available for this important step in the Next
Generation Sequencing process.