MOESM1 of Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers
posted on 2019-11-21, 05:06authored byVictor Bodart-Santos, Luiza Carvalho, Mariana Godoy, André Batista, Leonardo Saraiva, Luize Lima, Carla Abreu, Fernanda Felice, Antonio Galina, Rosalia Mendez-Otero, Sergio Ferreira
Additional file 1: Figure S1. Characterization of human Wharton’s Jelly mesenchymal stem cells (hMSCs). a MSCs had fibroblast-like morphology and stemness was confirmed by adipogenic (b, Oil Red O labeling) or chondrogenic differentiation (c, Alcian blue labeling). Scale bars, 50 μm. MSC phenotype was further confirmed by flow cytometry. Cells maintained in control medium (d) or serum-free medium (e) for 24 h were immunolabeled with antibodies against HLA-DR, CD14, CD34, CD45, CD73, CD90, CD105 or CD146. Green traces correspond to fluorescence intensities of markers, while purple shaded curves indicate isotypic controls.
Funding
Conselho Nacional de Desenvolvimento Científico e Tecnológico