Additional file 1: Figure S1. of Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291

(A) Chromatogram from size exclusion chromatography (SEC) run performed on a Superdex 200 Increase 10/300 GL column connected to an ÄKTA Pure 25 (GE Healthcare). The black line represents the calibration standard mix with molecular weights of standards labeled. The blue and red lines indicate the elution profile for cdPadR1WT and cdPadR1W94A, respectively (13.5 kDa monomer size for both). (B) Molar mass versus elution time of cdPadR1WT and cdPadR1W94A from SEC (as described) coupled with multi-angle light scattering (MALS) detection. Red lines indicate MALS signal (LS) and green lines indicate UV detection. cdPadR1WT and cdPadR1W94A both dimers with molecular weights (MW) of approximately 30 and 27 kDa, respectively (monomeric cdPadR1 MW is 13.5 kDa). (PPTX 222 kb)