CIL:11992, Drosophila melanogaster, epithelial cell
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Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (2021). CIL:11992, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08W3C11
- Description
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TIRF time lapse of mCherry-tubulin (left), myosin-GFP (middle), and overlay (myosin in green and tubulin in red; right) in a cell with a monopolar spindle from metaphase to anaphase. The S2 cells are treated with dsRNA to Klp61F (kinesin 5 motor) and BubR1 (checkpoint protein)). This video corresponds to the image in Fig. 5 A and video 9 from J Cell Bio 186:727-738, 2009.
Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org). - Date Issued
- 2021
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- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data; color combine
Data qualification: Raw;spatialmeasurements - Series
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- Identifier
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Samplenumber: 11992
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11992
- J Cell Bio 186:727-738, 2009 https://www.ncbi.nlm.nih.gov/pubmed/?term=19720876
- Vale RD, Spudich JA, Griffis ER. Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J Cell Biol. 2009 Sep 7;186(5):727-38. https://doi.org/10.1083/jcb.200902083
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
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- UC Regents
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12