Phytochemical Evaluation and In-Vitro Antibacterial Properties of the Methanolic Leaf Extract of Boswellia dalzielii Hutch. (Burseraceae)

Authors

  • Umar T Mamza Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeria
  • James Yakubu Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeria 3Department of Veterinary Physiology and Biochemistry, University of Maiduguri, Borno State, Nigeria
  • Mbursa Chiroma Department of Veterinary Physiology and Biochemistry, University of Maiduguri, Borno State, Nigeria
  • Victor M Balami Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeria 3Department of Veterinary Physiology and Biochemistry, University of Maiduguri, Borno State, Nigeria
  • Samaila Moses Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeri
  • Olufunke A Sodipo Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria
  • Fannah I Abdulrahma Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeria
  • Taiwo E Alemika Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Nigeria
  • Irfan Z Khan Department of Pure and Applied Chemistry, University of Maiduguri, Borno State, Nigeria

DOI:

https://doi.org/10.48165/

Keywords:

Phytochemical Evaluation, Antibacterial property, Boswellia dalzielii, leaf extract, in-vitro

Abstract

The upsurge of multi-drug resistance by microbes is at alarming rate and poses serious challenges in the field of  medical and pharmaceutical industries. This study was aimed to determine the active components and assess the  antibacterial potency of methanolic leaf extract of Boswellia dalzielii. The plant specimen was collected, identified,  prepared and cold macerated using 95% of methanol as solvent. The resulted crude extract was further assayed  for anti-bacterial potency; adopting agar well diffusion protocol against some selected human pathogenic isolates  viz S. aureus, S. pyogenes, B. Subtilis, Corynebact. Spp. K. pneumoniae, S. typhi,P. aeruginosa and E. coli. The Minimum  Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using the  standard protocol of microdilution assay. The antimicrobial assay of the crude extract showcased that B. dalzielii leaf is susceptible against all the tested pathogens by exhibiting significant degrees of inhibition zone of diameter  (0.25±0.26to 27.16±0.76 mm). The positive control (ciprofloxacin) showed superior activity against the tested  isolates (27.06±0.35 to31.03±0.64 mm). The MIC/MBC was observed at 25.0 to 6.25 mg/mL and 25.0 to 12.5  mg/mL against bacterial studied. S. aureus is the most susceptible pathogen with MIC/MBC values at 6.25/12.5 mg/ml respectively while S. Pyogene, B. subtilis, P. aeruginosa were inhibitory at 12.5 mg/ml and bactericidal at  25mg/ml. Corynebact. Spp. and K. pneumoniae had the moderate MIC/MBC at 25mg/ml. The phytochemical  analysis revealed the presence of alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, resins,  tannins and carbohydrates. This study showed that B. dalzielii leaf has a strong antibacterial activity against Gram +ve and Gram -ve bacteria tested; this provides a scientific justification to the traditional healers for the  utilization of the plant in the management of different ailments caused by the tested pathogens. 

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Published

2021-12-15

Issue

Vol. 40 No. 2 (2021): Bulletin of Pure and Applied Sciences Chemistry (Jul-Dec) 2021

Section

Articles

How to Cite

Phytochemical Evaluation and In-Vitro Antibacterial Properties of the Methanolic Leaf Extract of Boswellia dalzielii Hutch. (Burseraceae) . (2021). Bulletin of Pure and Applied Sciences-Chemistry , 40(2), 48–56. https://doi.org/10.48165/