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2014, vol. 43, br. 4, str. 93-97
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Antimikrobna aktivnost i HPLC analiza ekstrakata biljne vrste Daphne blagayana L. (Thymelaeceae)
Antimicrobial activity and HPLC analysis of Daphne blagayana L. (Thymelaeceae) extracts
aUniverzitet u Kragujevcu, Fakultet medicinskih nauka, Srbija bUniverzitet u Kragujevcu, Agronomski fakultet, Čačak, Srbija cUniverzitet u Nišu, Prirodno-matematički fakultet, Srbija
e-adresa: sofke-ph@hotmail.com
Projekat: Dinamika nelinearnih fizičkohemijskih i biohemijskih sistema sa modeliranjem i predviđanjem njihovih ponašanja pod neravnotežnim uslovima (MPNTR - 172015)
Sažetak
Cilj ovog rada bio je ispitivanje antimikrobne aktivnosti i HPLC analiza matanolnih i hloroformskih ekstrakata listova i grančica endemske biljne vrste Daphne blagayana, prikupljene sa područja Suve planine u Srbiji. Antimikrobna aktivnost, izražena preko vrednosti MIC, ispitana je na 6 standardizovanih bakterijskih sojeva i dva soja gljivica. Amracin i ketokonazol su korišćeni kao standardni antibiotici. Dobijene MIC vrednosti su se kretale u opsegu od 15,62 do 125 μg/mL, pri čemu su ekstrakti najjače dejstvo ispoljili prema vrstama, Klebsiella pneumoniae, Staphylococcus aureus i Proteus mirabilis. Rezultati HPLC analize pokazuju prisustvo više različitih sekundarnih metabolita, medju kojima je i 7,8-dihidroksi kumarin (dafnetin), koji je identifikovan u svim ispitivanim ekstraktima. Dafnetin, kao biološki aktivna supstanca, je velikim delom odgovoran za ispoljenu antimikrobnu aktivnost ekstrakata, ali se ne može isključiti ni uticaj ostalih sekundarnih metabolita (fenola, flavonoida) prisutnih u ispitivanim ekstraktima, koji mogu imati i sinergističko dejstvo.
Abstract
The aim of this study was to investigate the antimicrobial activity and HPLC analysis of the methanol and chloroform extracts of leaves and twigs of endemic species Daphne blagayana collected from the area of Suva Mountain, Serbia. The antimicrobial activity, expressed as the values of MIC, were investigated on the 6 standardized bacterial strains and two strains of fungi. Amracin and ketoconazole are used as standard antibiotics. MIC values of the tested extracts were ranged from 15.62 to 125 μg/ml. The strongest effect of the extracts exhibited against Klebsiella pneumoniae, Staphylococcus aureus and Proteus mirabilis. Results of the HPLC analysis showed the presence of a variety of secondary metabolites, one of which is 7,8-dihydroxycoumarin (daphnetin), which was identified in all the extracts tested. Daphnetin, as biologically active substance, is most responsible for the antimicrobial activity of the tested extracts, but its role can not exclude the influence of other secondary metabolites (phenols and flavonoids) present in the extracts, which may have a synergistic effect.
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