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Journal on Processing and Energy in Agriculture
2017, vol. 21, br. 2, str. 76-80
Antimikrobna aktivnost kompozitnog hitozanskog filma sa dodatkom pčelinjeg voska i etarskog ulja kima
Hromiš Nevena ORCID, Lazić Vera, Bulut Sandra ORCID, Popović Senka ORCID, Šuput Danijela ORCID, Markov Siniša ORCID, Džinić Natalija, Tomović Vladimir ORCID
Univerzitet u Novom Sadu, Tehnološki fakultet, Srbija

e-adresa: nevena.krkic@uns.ac.rs
Projekat:
Razvoj tradicionalnih tehnologija proizvodnje fermentisanih suvih kobasica sa oznakom geografskog porekla u cilju dobijanja bezbednih proizvoda standardnog kvaliteta (MPNTR - 31032)

Sažetak
Antimirobno pakovanje hrane, kao jedna od osnovnih aplikacija sistema aktivnog pakovanja, treba da ima mogućnost da onemogući ili inhibira rast mikroorganizama koji izazivaju kvar hrane, kao i patogenih mikroorganizama i, na ovaj način, osigura bezbednost, održi kvalitet i produži održivost hrane. U ovom radu, ispitan je uticaj dodatka rastuće koncentracije pčelinjeg voska na antimikrobnu aktivnost kompozitnog filma na bazi hitozana sa dodatkom etarskog ulja kima. Kao test mikroorganizmi odabrani su Salmonella Typhimurium i Listeria monocytogenes. Aktivnost je određena prema ASTM E2149 metodi, koja predstavlja kvantitativnu metodu određivanja antimikrobne aktivnosti koja se izvodi pod dinamičkim uslovima kontakta. Redukcija vijabilnih ćelija u suspenzijama S. Typhimurium i L. monocythogenes u prisustvu hitozanskog filma sa etarskim uljem kima, kao i u prisustvu filmova sa pčelinjim voskom iznosila je preko 80% nakon 24h kontakta, odnosno 95% nakon 3h kontakta i uticaj dodatka pčelinjeg voska na redukciju nije bio uočljiv. Sa druge strane, ukoliko se analizira broj vijabilnih ćelija (log CFU/ml), a ne redukcija vijabilnih ćelija (%), može se videti da je dodatak pčelinjeg voska ipak doveo do povećanja aktivnosti filma, posebno u slučajevima sa dodatkom 36 kg/m3 i 54 kg/m3 voska, gde je inhibicija rasta S. Typhimurium iznosila 1.9 log jedinica i 1,6 log jedinica, dok je za film bez dodatog voska ova redukcija iznosila 0,7 log jedinica. Kada su filmovi premešteni, nakon 24 h u bakterijskoj suspenziji, na Petri ploče sa nutrient agarom i inkubirani tokom 24 h na 37°C, rezultati su takođe pokazali doprinos pčelinjeg voska inhibiciji rasta S. Typhimurium.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/JPEA1702076H
objavljen u SCIndeksu: 12.06.2017.

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