Correlation between Exercise Stress Test and Echocardiographic Parameters in Elderly Individuals

Mailing Address: Liz Andréa Villela Baroncini Rua Buenos Aires, 764, ap. 601. Postal Code: 80.250-070, Batel, Curitiba, PR Brazil. E-mail: lizavb@cardiol.br, lizandreabaroncini@hotmail.com Correlation between Exercise Stress Test and Echocardiographic Parameters in Elderly Individuals Liz Andréa Villela Baroncini,1 Camila Varotto Baroncini,2 Juliana Ferreira Leal3 Pontificia Universidade Católica do Paraná,1 Curitiba, PR Brazil Universidade Federal do Paraná (UFPR),2 Curitiba, PR Brazil Universidade Estadual de Ponta Grossa, Ponta Grossa,3 PR, Brazil


Introduction
Priebe 1 provided a sensible description of the difficulties in defining "aged patients", since there is no clinical definition that precisely classifies elder or advanced-aged individuals.Aging is a continuous process rather than an abrupt event.As age advances, maximal aerobic capacity decreases 8 to 10% per decade in sedentary men and women, and exercise capacity decreases approximately 50% between ages 30 and 80.[4] The prevalence of coronary artery disease (CAD) is high in the elderly.[7] Older patients require a special and careful approach.Functional capacity is evaluated by exercise tolerance in daily life and reflects the quality of biological age.Lower exercise tolerance may reflect the severity of an underlying disease such as significant CAD or just poor functional capacity in a sedentary old person.An individual's functional capability may be assessed by means of the maximum oxygen uptake (VO 2 max) that represents the maximum amount of oxygen an individual can take in with incremental exercise.The amount of exercise can be measured using the metabolic equivalent (MET); 1 MET is the amount of oxygen consumption at rest and is equivalent to approximately 3.5 ml kg - 1 min -1 (measured in a healthy, 40-year old man, 70 kg).VO 2 max decreases about 10% per decade in healthy individuals, and such decrease is even more pronounced in individuals older than 70 years.With the increase in life expectancy, many patients aged 75 years or older seek medical care for chest pain and presurgical evaluation for several elective surgeries.Individuals that feel fit enough to perform a physical stress test are submitted to treadmill or bicycle ergometric tests.However, although sensitivity to noninvasive stress testing increases with aging, specificity tends to decline. 5e objective of the current study is to correlate exercise test variables with echocardiographic parameters in patients over 75 years old, including functional capacity, measured in MET and VO 2 max (with or without myocardial ischemia at the physical stress test), left ventricular ejection fraction (LVEF), left ventricular mass and left ventricle mass index, left atrial volume and presence of pulmonary arterial hypertension.

Methods
We assessed 381 patients (205 women; 53.8%), mean age of 79 ± 3.7 years, who underwent exercise test and bidimensional transthoracic echocardiography (2DEcho) in a private cardiologic clinic.Subjects were selected by convenience.Each patient had results of blood tests and imaging tests to be analyzed before the exercise test.
Before the study, data on demographic characteristics and risk factors were collected from the private cardiologist's records and blood test results.Body mass index (BMI) was calculated by dividing the subjects' weight (kg) by the square of their height (m).Patients were queried about the presence of hypertension, diabetes mellitus, dyslipidemia, coronary artery disease, and current smoking habit.Hypertension was defined as a history of treated hypertension or the presence of systolic blood pressure ≥ 140 mmHg or diastolic blood pressure ≥ 90 mmHg, measured by the private cardiologist.Smoking history was coded as never or current smoker. 8Subjects were classified as having diabetes when treated for insulin-dependent or noninsulin-dependent diabetes or having elevated fasting glucose levels (≥ 126 mg/dL).The use of lipid-lowering drugs or the presence of total cholesterol > 200 mg/dL, HDL-cholesterol < 40 mg/dL, LDL -cholesterol > 100 mg/dL or triglycerides > 150 mg/dL was recorded. 9-10A history of myocardial infarction, angioplasty, or coronary artery bypass surgery was recorded, and the presence of any of these conditions was considered a positive CAD history.
Indications for the 2DEcho included referral from a physician, information from close relatives, or patients' complaints.We analyzed echocardiographic and carotid ultrasonography data, including left ventricular ejection fraction, left ventricular diastolic function, left atrial volume, left ventricular mass and the presence of pulmonary arterial hypertension and carotid plaque.Exclusion criteria included the presence of left ventricular systolic dysfunction (ejection fraction < 50% on echocardiogram), left ventricular diastolic dysfunction grade II and III, significant valve disease such as mitral and aortic regurgitation or stenosis, CAD with left ventricular systolic dysfunction or dilatation, unstable cardiovascular or metabolic disease, and major orthopedic/neurological disability.
Subjects underwent treadmill electrocardiogram (ECG) testing (TET) or bike ECG testing (BET), according to the private physician request.Treadmill ECG test included Ellestad, Kattus, Naughton, Ramp, Bruce and modified Bruce protocols, and Balke and male Balke protocols, following standard recommendations. 11,12The distance covered on the treadmill was automatically calculated by the protocol, according to the number of laps covered by each patient.Blood pressure and a 12-lead ECG were recorded before the test, during the test (during the last minute of each stage), and every 3 minutes in the recovery phase.During the test, three ECG leads were continuously monitored.The test was stopped in case of a) ST-segment elevation (> 1.0mm) in leads without preexisting Q waves due to prior myocardial infarction (other than aVR, aVL, and V1); b) drop in systolic blood pressure > 10 mmHg despite an increase in workload, when accompanied by any other evidence of ischemia; c) moderate to severe angina; d) central nervous system symptoms (e.g.ataxia, dizziness, near syncope); signs of poor perfusion (cyanosis or pallor); e) sustained ventricular tachycardia or other arrhythmias, including second-or third-degree atrioventricular block, which may affect cardiac output during exercise; f) marked ST-segment depression (≥ 3mm); g) exercise-limiting symptoms such as angina, dyspnea, exhaustion, or the subjects' request to stop the test; and h) technical difficulties in monitoring the ECG or systolic blood pressure.An abnormal response of the ST-segment to exercise was defined as horizontal or downsloping ST-segment depression ≥ 1 mm measured at 80 ms after the J point or an elevated ST-segment ≥ 1 mm in leads without pathological Q-wave (excluding lead aVR).4] The study was approved by the local ethics committee and written informed consent was obtained from each participant to undergo the ergometric tests (treadmil ECG testing or bike ECG testing) , bidimensional transthoracic echocardiography and carotid ultrassonography, and to participate in the study.

Statistical analysis
Quantitative variables were described as means, medians, minimum and maximum values, quartiles and standard deviations, and categorical variables as frequency and percentiles.Associations between quantitative variables were analyzed by Pearson and Spearman correlation coefficients.Comparisons of quantitative variables between the two groups were made using the Student's t test for independent samples.Statistical testing of data normality was performed using the Kolmogorov-Smirnov test.Associations between categorical variables were assessed by the Fisher's exact test.A p-value ≤ 0.05 indicated statistical significance.Data were analyzed by means of the SPSS statistical software, version 20.

Results
Patients' baseline characteristics and echocardiographic and ergometric results are shown in Tables 1, 2 and 3.Only five patients (1.3%) performed cycle ergometer test, and then were excluded from the final analysis.Three hundred seventy-six patients performed treadmill test (Bruce protocol 203, 53.4%; Kattus 113, 29.7%; Ramp 28, 7.4%; modified Bruce 15, 3.9%; Naughton 12, 3.2%; Ellestad 5, 1.3%; Balke 3, 0.8%; Balke male 1, 0.3%).Nineteen (5%) patients did not achieve the submaximal heart rate (HR) expected for the age and 58 (15%) had previous ECG at resting conditions showing left bundle branch block and ST segment alterations.Forty (10.5%) of the patients tested positive for myocardial ischemia and 79 (21.8%) showed abnormal heart rate response in the first minute.As age increased, the distance covered by participants decreased (p = 0.021), as well the expected increase in HR (p < 0.001), VO 2 max (p < 0.001) and METs (p < 0.001) (Tables 3 and 4; Figure 1) in men and women.Women showed lower values of VO 2 max and METs when compared to men (Table 2).Inverse correlation was noted of the distance covered, VO 2 max and METs with the BMI (Table 3 and 4).Only 4 patients (1%) showed systolic pressure in the pulmonary artery above 40 mmHg in the echocardiogram at rest, which did not influence the distance covered by the subjects, HR at the first minute (p = 1), VO 2 max (p = 0.5), MET (p = 0.5) or ischemia (p = 1.0) (data not shown).The volume of the left atrium and left ventricular mass had no influence on the ergometric test variables (Table 5).Ischemia at stress test did not correlate with any echocardiographic variable (Table 5).In 198 patients (67.3%), atherosclerotic plaques in the extracranial carotid arteries were detected, which also did not correlate with any of the variables analyzed (data not shown).Severity of stenosis was not considered relevant, only the presence of the atherosclerotic plaque.

Discussion
The present study showed that relatively healthy patients aged 75-81 years, with similar demographic and echocardiographic characteristics, showed a progressive decrease in METs and VO 2 max, associated with a decrease in the distance covered during ergometric test with increasing age.6][17][18] Considering that only individuals with preserved left ventricular systolic function was studied, we did not expect an influence of this parameter on the results.Similarly, no influence of left ventricular diastolic function was expected, 19 as individuals with grade II and III diastolic dysfunction were excluded from the study.
1][22][23] The same was observed with left ventricular mass and left ventricular mass index. 24][27][28] Nevertheless, comorbidities such as previous stroke, bone and articular diseases, and chronic obstructive pulmonary disease were not analyzed in the present study.0][31] In this regard, in the present study, only 21.8% of the individuals showed an abnormal HR response at the first minute after the exercise test.Also, there was no correlation of this variable with echocardiographic parameters, age, sex or BMI.This finding was expected, as the studied cohort comprised an aged population with similar clinical and echocardiographic characteristics.Another relevant finding was the fact that only 10% of the individuals in the present study showed positive for myocardial ischemia.Sensitivity of the ergometric test was similar to that documented by Vacanti et al., 6 using myocardial perfusion scan with dipyridamole in individuals older than 75.However, it is known that elderly patients have a high prevalence of severe CAD, with low tolerance to exercise.Thus, results of exercise stress testing in    this population must be interpreted differently than in younger individuals, since even in patients classified as low risk by risk stratification scores, an annual cardiac mortality rate of 2% was found in patients aged 75 years or older. 32,33These findings confirm the need for specific protocols and instruments for elderly patients, 34 considering the great heterogeneity in aging process and its biological consequences. 33 addition, considering the presence of atherosclerotic plaques in the extracranial carotid arteries in our patients, we expected its correlation with the other variables analyzed, which did not happen.In fact, its presence was previously shown to be correlated with systolic functions and left filling ventricular pressures,which revealed to be similar in all patients of this study.
An additional important finding was the inverse correlation of the distance covered, functional capacity (METs) and VO 2 max with BMI.There is a progressive BMI increase as age advances and the prevalence of obesity has considerably increased in the elderly. 36his has a direct impact on individuals' health and life quality, since weight gain is associated with a decrease in functional capacity and vitality, body pain, emotional and physical problems, and increased risk for morbidity and disability. 37,38me limitations of the present study should be mentioned.First, the choice of the exercise protocols was made by the physician who examined the patients, based on the physical limitations of each patient.This led to the use of different ergometric protocols, making

Conclusions
Individuals aged 75 years or older, of both genders, relatively healthy, with preserved left ventricular systolic and diastolic functions, showed progressive decrease in the distance covered, VO 2 max, METS and at the expected increase in HR in exercise stress ECG test, due to aging and related comorbidities and physical deconditioning.

Figure 1 -
Figure 1 -Correlation of age with heart rate, VO 2 max, metabolic equivalent (MET), and distance (m) covered in exercise stress ECG test.

Table 5 -Echocardiographic variables, presence of ischemia and heart rate in the first minute of the exercise stress ECG test (HR 1st min) of the patients
Baroncini et al.Ergometric test and echocardiography in the elderly Int J Cardiovasc Sci.2018; [online].aheadprint,PP.0-0 Original Articleit difficult to accurately analyze and compare the ergometric variables between the subjects.Second, since only patients with preserved left ventricular systolic and diastolic functions were selected, no significant difference was expected in VO 2 max, METs, HR, and distance covered.Thus, further studies including patients with different degrees of left ventricular dysfunction in the elderly are necessary.