Ficusonic Acid : a New Cytotoxic Triterpene Isolated from Maytenus royleanus ( Wall . ex

A investigação fitoquímica das raízes de Maytenus royleanus resultou no isolamento de um novo triterpeno citotóxico, denominado ácido ficusônico, ou ácido 3β,21β-diidroxiolean-12-en29-óico, juntamente com três compostos conhecidos, ácido 3α,22β-diidroxiolean-12-en-29-óico, ácido salaspérmico e ácido ortosfênico, relatados pela primeira vez nesta espécie. Suas estruturas foram estabelecidas com base em técnicas espectroscópicas. A atividade citotóxica do composto ácido 3β,21β-diidroxiolean-12-en-29-óico foi avaliada contra duas linhagens de células de câncer: PC-3 (próstata) e HeLa (cervical). O ácido 3β,21β-diidroxiolean-12-en-29-óico apresentou fraca atividade contra PC-3 (IC50 = 35,42 μmol L ), todavia contra HeLa (IC50 = 20,47 μmol L ) sua atividade foi moderada.


Introduction
The family Celastraceae (also called Chingithamnceae, Canotinceae, Goupiaceae and Siphonodentaceae) is a large family of 90-100 genera and about 1300 species which are widely distributed in the world having a wide range of uses in folk medicine. 1In China and South America, species of Celastraceae have been used for the treatment of stomach disorder, fever, cancer, arthritis and as insecticidal. 2,3Particularly, the genus Maytenus is used as insecticide, 4 anticancer, 5 for cure of skin problems and rheumatism, 6,7 and in Canary Island, it has been applied by shepherds for extreme fatigue. 8he species of the genus Maytenus (Celastrales order, Celastraceae family) have proven to be a rich source of the structurally diverse cytotoxic compounds: maytensinoids, 9 quinoid triterpenes, [10][11][12] sesquiterpene polyesters 13 and sesquiterpene pyridine alkaloids. 14Maytenus royleana is a widely distributed thorny shrub of Pakistan Northern region and commonly known as "sur azghee".Literature survey reports no previous phytochemical study of this plant.In this work, it is reported the isolation of a new triterpene 3β,21β-dihydroxyolean-12-en-29-oic acid (1), which was named ficusonic acid, together with three known compounds, 3α,22β-dihydroxyolean-12-en-29-oic acid (2), 15 salaspermic acid (3) 16 and orthosphenic acid (4). 17aytenus royleana was collected, ground and processed (vide experimental), however for the bioassay and dereplication purpose, small quantity of the plant (200 g) was extracted with methanol.The crude methanolic extract was partitioned into different fractions of hexane, dichloromethane and methanol.In the preliminary screening for antiproliferative activity, the dichloromethane fraction (FB) was found cytotoxic which become prelude for our future study.Large scale extraction of Maytenus royleana (13 kg)   1).

Results and Discussion
The known data was dereplicated by comparing the UV and MS (mass spectra) data with reported compounds in Dictionary of Natural Product (DNP) database. 18A number of hits was observed in DNP for four major peaks (m/z 472, 472, 472 and 488 having different UV values and retention time) in the LC-MS (liquid chromatography-mass spectrometry) profile of the FB fraction.However due to excellent activity of crude fractions, the peaks were selected for purification and characterization.
The structure of ficusonic acid (1) commenced by establishing of the molecular formula C 30 H 48 O 4 through HREIMS (high resolution electron impact mass spectroscopy) m/z 472.3602 (calcd.472.3553 for C 30 H 48 O 4 ).The IR spectrum showed absorption bands at 3358 (hydroxyl), 3045 (C-H olefinic), 1760 (carboxylic carbonyl) and 1381 (gem-dimethyl group) cm -1 . 13C Nuclear magnetic resonance (NMR) and distortionless enhancement by polarization transfer (DEPT) spectra indicated a total of 30 carbons including seven tertiary methyls, nine methylenes, six methines (three sp 3 hybridized, two oxymethines at d 76.0 and 79.7 and one olefinic carbon at d 124.3) and eight quaternary carbons (six sp 3 hybridized, one olefinic d 144.7 and one carboxylic carbon at d 182.3) (Table 1).In the 1 H NMR spectrum, one proton triplet at d 5.25 (J 3.5 Hz, H-12) displayed the presence of a trisubstituted double bond and two oxygenated methines resonated at d 3.14 (dd, J 11.5, 4.5 Hz, H-3) and 3.51 (dd, J 12.5, 4.5 Hz, H-21) (Table 1).The 1 H NMR spectrum showed seven tertiary methyl signals as expected for olean-12-en skeleton with a secondary hydroxyl substituent. 18The characteristic pentacyclic triterpene fragmentation pattern was observed in the EIMS spectrum of 1 (Figure 2a).9][20] The 7 degree of unsaturations evident in the molecular formula was satisfied by one carbonyl group, one olefinic bond and five rings.
The relative configuration of two hydroxyl groups at C-3 and C-21 were determined by splitting pattern, coupling constant and NOESY (nuclear Overhauser effect spectroscopy) correlation spectrum.Both the carbinol protons in compound 1 appearing as doublet of doublet (dd, 3.14, J 11.5, 4.5 Hz, H-3 and dd, 3.51, J 12.5, 4.5 Hz, H-21) revealed the equatorial positions of two hydroxyl groups at C-3 and C-21 instead of triplet for axial position.The splitting pattern and large coupling constant of the signal at d 3.14 (dd, J 11.5, 4.5 Hz, H-3) suggested a β-configuration for hydroxyl group at C-3, otherwise it would appeared as a broad singlet. 19The β-configuration of the two hydroxyl groups was deduced from NOESY correlation spectrum; the proton at d 3.14 (H-3) correlated with protons at d 0.96 (H-24) and d 1.A detailed analysis of the NMR spectral data and comparison with related compounds in the literature [18][19][20]   suggested that the structure of 1 as 3β,21β-dihydroxyolean-12-en-29-oic acid (ficusonic acid).
Ficusonic acid (1) was tested against two cancer cell lines, HeLa (cervical cancer cells) and PC-3 (prostate cancer cells) for its antiproliferative activity along with three known compounds 2-4.The activity of the four compounds 1-4 against PC-3 was weak with IC 50 values 35.42, 35.61, 34.46 and 40.42 μmol L -1 , respectively (Table 2); however in case of HeLa compounds 1 and 3 showed moderate or significant activity with IC 50 values 20.47 and 22.60 μmol L -1 , respectively.

General experimental procedures
Melting point was determined on Buchi 535 apparatus and the IR data (KBr) were taken on Bruker Vector 22 spectrophotometer, ν max in cm -1 .Optical rotation analysis was recorded on Jasco-P2000 digital polarimeter in MeOH at room temperature.UV data was taken from 996 photodiode array detector connected to LC-MS instrument. 1 H NMR (500 MHz, CD 3 OD) and 13 C NMR (125 MHz, CD 3 OD) spectra were recorded on Bruker 500, while the chemical shift values were presented in ppm (d) and coupling constant (J) in Hz.For dereplication analysis, the LC-MS profile of FB was developed by HPLC-ELSD-UV-MS system (column C 18 ; solvent system: acetonitrile and water (0.1% formic acid in both); gradient system: 10-100% for 30 min).The LC-MS data for confirming the purity of the compounds (MeOH/H 2 O with 0.1% FA) was recorded by using a Phenomenex Luna column C 18 RP (5 μm, 150 × 4.6 mm) on Sedex 55 ELSD (evaporative light-scattering detector), 996 photodiode array detector in combination with ESI-TOF-MS (+) (time-of-flight electrospray ionization mass spectrometry).During purification on HPLC, the column used was Phenomenex 5μm Luna C 18 RP column (250 × 10 mm).ESI (+ve) MS and HRESIMS (high resolution electrospray ionization mass spectrometry) spectra were recorded on mariner ESI-TOF-MS (+).For open gravity column and thin layer chromatography, silica gel 60 column, mesh size 70-230 (E.Merck, 0.063-0.200mm) and silica gel 60 PF254 (E.Merck) were used, respectively.

Plant material
The roots of Maytenus royleanus were collected from the Buner district, Khyber Pukhtoonkhwa, Pakistan during the month of June 2008.The plant was identified by taxonomist Mr. Ambara Khan (Degree College Daggar, Buner).The voucher specimen (Bot.10068) was deposited in the Herbarium of Department of Botany, University of Peshawar, Khyber Pukhtoonkhwa (KPK), Pakistan.

Extraction and isolation
The air dried roots (13 kg) of Maytenus royleanus were repeatedly extracted (X3) with 80% MeOH/H 2 O at room temperature after every 24 h.The combined extract was concentrated under vacuum at 40 °C, to obtain brownish thick syrup that constituted the crude aqueous methanolic extract (100 g).This was first partitioned according to our standard laboratory procedure into five fractions: FA, FB, FC, FD and FE.The crude extract suspended in distilled water and defatted (X3) with petroleum ether afforded fraction FA (35 g).The polarity of aqueous phase was changed to 10% MeOH/H 2 O by addition of MeOH (200 mL) followed by extraction with dichloromethane (DCM) and concentrated under vacuum to obtain DCM FB (11.5 g) was subjected to column chromatography (CC) on silica gel 60 (70-230 mesh, Merck) and eluted with solvents n-hexane and ethyl acetate increasing in order of polarity.As a result, seven sub-fractions (FD1F1 to FD1F7) were obtained after combination of the fractions on the basis of TLC profile.Sub-fraction FD1F6 (125 mg) was subjected to further CC on silica gel 60 (70-230 mesh, Merck), n-hexane, n-hexane-EtOAc, EtOAc solvent systems and elution with 23% n-hexane/EtOAc furnished 4 (22 mg).Fraction FD1F3 was a mixture of three compounds based on LC-MS profile which was subjected to HPLC (Phenomenex Luna column C 18 RP (5 μm, 150 × 4.6 mm; 0.1% acidic (formic acid) gradient solvent system (10-100 MeCN/H 2 O in 30 min) furnished three compounds; 1 (8 mg), 2 (5 mg) and 3 (20 mg) (see Figure S1b in the Supplementary Information (SI) section).1.

Antiproliferative assays
Antiproliferative results of the compounds 1-4, were determined by the MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5diphenyltetrazolium bromide] assay on two different cancer lines.HeLa (cervical cancer lines) and PC-3 (Prostate cancer lines) are shown in Table 1.Cells were grown in DMEM (Dulbecco's modified eagle medium) for PC-3 and MEM (minimal essential medium) for HeLa, containing 10% FBS (Fetal Bovine Serum) and 2% antibiotic (penicillin and streptomycin) and maintained at 37 o C with 5% CO 2 level for 24 h in flask.Cells (1 × 10 5 cells mL -1 ) were placed in a 96 well flat bottom plates for 24 h incubation to allow for cell attachment.Various concentrations of sample varying from 100-1 μmol L -1 were added into the well and incubated for 48 h.The IC 50 values were calculated and at least three independent experiments were carried out for each sample.Doxorubicin was used as positive control in this assay for both PC-3 and HeLa. 21

Table 1 .
13 and13C NMR data and HMBC correlations of compound 1 in CD 3 OD a