Renal inflammatory and oxidative and metabolic changes after 6 weeks of cafeteria diet in rats

Introdução: A obesidade é uma doença em que a inflamação está inteiramente envolvida e pode causar insuficiência renal. Objetivo: Avaliar a influência da exposição a curto prazo de uma dieta de cafeteria sobre a inflamação no tecido renal e a formação de produtos de glicação avançada (AGEs) no plasma de rato. Métodos: Ratos Wistar machos (10 semanas de idade, pesando 350 g) foram designados para receber dieta de ração comercial (C; n = 8 animais/grupo, 5% de energia a partir de gordura) ou dieta de cafeteria (CAF-D, n = 8 animais/grupo: 29% de energia de gordura) e de sacarose em água (300 g/L) de beber durante 6 semanas. Resultados: Índice de adiposidade em seis semanas foi maior no grupo CAF-D em comparação com C. O mesmo comportamento foi observado para os níveis plasmáticos de glicose, triglicerídeos, leptina, insulina e AGEs. A expressão do gene de IL-6 e TNF-α em tecido renal foi maior no grupo D-CAF e nenhuma diferença significativa no tecido adiposo. Não houve aumento destas citocinas no plasma ou rim. Houve uma diminuição significativa de adiponectina no grupo CAF-D. Conclusão: A exposição a curto prazo da CAF-D reflete alterações no metabolismo, aumento dos níveis plasmáticos de AGEs, o que pode refletir o aumento expressão de citocinas inflamatórias no rim. resumo

Introduction: Obesity is a disease in which inflammation is directly involved and can lead to impaired renal function.Objective: To evaluate the influence of short term exposure to cafeteria diet on kidney tissue inflammation and advanced glycation end products (AGEs) in the rat plasma.Methods: Male Wistar rats (10 weeks of age, weighing 350 g) were assigned to receive commercial chow diet (C; n = 8 animals/group, 5% of energy from fat) or cafeteria diet (CAF-D, n = 8 animals/group: 29% energy fat) and sucrose in drinking water (300 g/L) for 6 weeks.Results: adiposity index at six weeks was higher in CAF-D group compared to C. The same behavior was observed for plasma levels of glucose, triglycerides, leptin, insulin and AGEs.The gene expression of IL-6 and TNF-α in renal tissue was higher in CAF-D group and no significant difference in adipose tissue.There was no increase of these cytokines in plasma and kidney or histologically.There was a significant decrease of adiponectin in the CAF-D group.Conclusion: The short exposure CAF-D reflects changes in metabolism, increased plasma levels of AGEs, which may reflect the increased expression of inflammatory cytokines in the kidney.consumption becomes chronic, there is an imbalance in the redox system, leading to the generation of these species, which are associated with metabolic disorders and inflammatory damage in several organs. 4,5GEs may act in several ways, among them, by binding to the RAGE receptor (receptor for advanced glycation end products), starting a cascade of events involving signal transduction kinases.This association culminates in the activation of factors Ikkβ/NFkB-nuclear transcriptional elements involved in the production of pro-inflammatory cytokines. 6As these receptors are present in various organs, including the kidneys, this can be a way of obese individuals develop kidney failure. 78][9] However, the literature does not show if a short period of intake of sugar and fat would be able to enhance the formation of AGEs, which leads to metabolic changes and inflammation in the kidney tissue.So, the aim of this study was to evaluate the influence of the short period of exposure cafeteria diet in inflammation of the kidney tissue as well as AGEs in plasma of rats.

mAterIAls And methods animals and experimental prOtOcOl
Male Wistar rats (10 weeks old, weighing nearly 350 g), from the Animal Center of Botucatu Medical School, Sao Paulo State University, UNESP (Botucatu, SP, Brazil), were initially divided to receive either a standard control diet (5% energy from fat and water (C, n = 8 animals/group) or cafeteria diet (29% energy from fat) and sugar in the drinking water (300 g/l) (CAF-D, n = 8 animals/group) during 6 weeks.The cafeteria diet was designed in our laboratory to contain a powdered commercial chow diet -NUVILAB CR-1 (Nuvital Q5), plus processed foods how wafer biscuit, condensed milk and palm oil, vitamins and minerals.The nutritional composition of the diets is presented in Table 1.
Rats were housed in individual cages in an animal facility at the Internal Medicine Experimental Laboratory, Botucatu Medical School, UNESP, under a controlled ambient temperature (22-26 °C) and lighting (12 h light-12 h dark cycle) condition.The animals were killed by decapitation after fasting 12 hours and anesthesia with sodium pentobarbital Q4 (50mg/kg, intraperitoneal injection).The experiment was conducted in accordance with the Guidelines for the Care and Use of Experimental.Animals and the diets followed the specifications from Nutrient Requirements of the Laboratory Rats.The protocol was approved by the local Ethical Committee for Animal Research (protocol nº PE-47/2011).

Adiposity index
The adiposity index (AI) was used as an indicator of obesity because it enables the precise evaluation of body fat percentages.Epididymal, retroperitoneal, and visceral fat deposits were dissected from the rats.The sum of the fat deposits, normalized by body weight [(epididymal + retroperitoneal + visceral)/body weight] x 100, was calculated to obtain the adiposity index. 10asma measurements An enzymatic colorimetric kit was used to measure glucose (Bioclin ® , Belo Horizonte, Minas Gerais, Brazil), triglycerides and cholesterol (Bioclin ® , Belo Horizonte, Minas Gerais, Brazil).Spectrophotometry was performed with the Chemistry Analyser BS 200 automatic spectrophotometer (Mindray Medical International Limited, China).Insulin, leptin, adiponectin and AGEs were purchased from Millipore Corporation, USA.TNF-α and IL-6 from R&D System, USA.Plasmatic levels were measured using ELISA methods according to manufacturer's instructions using a microplate spectrophotometer reader (SpectraMax 190, Molecular Devices, Sunnyvale, CA, USA).

insulin resistAnce
Insulin resistance was determined using the index of homeostasis model assessment (HOMA-IR) using the following formula: 11 HOMA-IR = fasting insulin (µU/ ml) x fasting glucose (mmol/l)/22.5.

plAsmA insulin And AGes meAsurements
Insulin (Millipore Corporation, Billerica, MA, USA), AGEs (Cell Biolabs, INC, San Diego, CA) plasma concentrations were measured by the ELISA method.A microplate spectrophotometer reader (SpectraMax 190, Molecular Devices, Sunnyvale, CA, USA) was used, according to manufacturer's instructions.
QuantificatiOn Of kidney gene expressiOn using real-time pcr fOr interleukin-6 (il-6) and tumOral necrOsis factOralfa (tnf-α) Total RNA was extracted from epididymal adipose and renal tissues using the reagent TRIzol (Invitrogen).The SuperScript II First-Strand Synthesis System for RT-PCR (Invitrogen) kit was utilised for the synthesis of 20ml of complementary DNA from 1000 ng of total RNA.The mRNA levels of TNF-a (assay Rn 00562055_m1; Applied Biosystems) and IL-6 (assay Rn 01410330_m1; Applied Biosystems) were determined by real-time PCR.Quantitative measurements were made with a commercial kit (TaqMan qPCR; Applied Biosystems) in a detection system (StepOne Plus; Applied Biosystems).Cycling conditions were as follows: enzyme activation at 50°C for 2min, denaturation at 958C for 10 min, complementary DNA products were amplified for forty cycles of denaturation at 95 °C for 15s and annealing/extension at 60 °C for 1 min.Gene expression was quantified in relation to the values of the C group after normalisation by an internal control (cyclophilin: assay Rn 00690933_m1; Applied Biosystems) by the method 22DDCT, as described previously. 12

HistoloGicAl AnAlysis
Renal tissue was fixed with 4% formaldehyde and embedded in paraffin.Two consecutive sections from each sample were cut (4 µm) and stained with hematoxylin-eosin.The whole-slides were scanned by 3DHISTECH Pannoramic MIDI System attached to HITACHI HV-F22 color camera and analyzed under 40X magnification in a blinded manner.

stAtisticAl AnAlyses
The Kolmogorov-Smirnov test was used to verify the normality of the data.Results are expressed as mean ± standard deviation (SD), and significance was calculated by t-test for independent variables.The software used was SigmaStat version 3.5 for Windows (Systat Software, Inc.).Differences were considered significant at p < 0.05.

results
Adiposity index of CAF-D animals in the sixth week was significantly higher compared to C animals (Figure 1).About the metabolic parameters, we observed that glucose, triglycerides, leptin and insulin were higher in CAF-D group compared to C group.We also observed that within six weeks the CAF-D animals developed insulin resistance (increased HOMA-IR) and significant decrease in plasma adiponectin.There was no difference in the levels of TNF-α and IL-6 between the groups (Table 2).The plasma concentration of AGE is presented in Figure 2. We observed increase in CAF-D animals compared to C animals.Besides, animals fed cafeteria diet showed a significant increase in gene expression of IL-6 and TNF-α in renal tissue (Table 3).This behavior was not observed in gene expression in adipose tissue (Table 3).
Histological analysis of the kidney in the two groups showed no structural alterations or inflammatory characteristics with absence of peritubular, glomerular or interstitial inflammatory infiltrate.Tubules and glomerulus showed normal cellular architecture.Morphology of vascular endothelium unchanged and no focal or disseminated spots of collagens deposition, which could suggest sclerosis.Normal glomerular hypercellularity, compatible with the strain of mice used, and similar to the control animals.
include increased levels of circulating cytokines, as well as glycemia, triglycerides, leptin and decreased of adiponectin. 14Our study observed the manifestation of obesity in 6 weeks, with animals showing higher adiposity index, increased fasting glucose, triglycerides, leptin, insulin resistance, and reduced adiponectin reflecting the context of obesity and metabolic disorders.These changes in body composition and metabolism are already established in the literature, but with longer periods of exposure to high fat diet as the work of Vincent et al., 15 which conducted a study observing metabolic changes weekly for 20 weeks in animals fed high-fat diet (45%) and only at 12 th week changes in lipid profile, plasma glucose and hormonal were founded.The difference in the metabolic events may be explained by the composition of the diet.High-fat diet consist in a diet in which most of the calories derived from fat, while cafeteria diet is made up of a combination of palatable foods, high energy density, which currently reflects the Western dietary pattern.In a study of Sampey et al., 16 which compared the effect of cafeteria diet and high-fat diet in inducing metabolic syndrome in rats, showed that cafeteria diet induced an increase in body weight and epididymal fat compared to high fat diet group and was also able to induce insulin resistance as early as seven weeks of consumption, what did no happen with the high fat diet.These data corroborate the cafeteria diet as a trigger of metabolic changes.
8][19] Obese people with chronic kidney disease have higher rate of decline in glomerular filtration rate and progress faster to the renal disease. 20However, the mechanisms by which obesity predisposes people to kidney damages are unknown. 8It is known that obesity causes increase in inflammation in the kidney tissue and renal injury 21 and one of the causes of this inflammation is oxidative stress. 22AGEs are generated in vivo as a normal consequence of metabolism, but their formation is accelerated under conditions of hyperglycemia, hyperlipidemia and increased oxidative stress. 7,23hese AGEs are highly reactive and can trigger inflammation by generating particularly TNF-α and IL-6, 8 which could damages the kidney via the tubular cell apoptosis, and can also promote the proliferation of mesangial cells generating mesangial proliferative glomerulonephritis, and nephritis. 24

dIscussIon
Obesity is accompanied by systemic inflammatory response and metabolic disorders. 13This process In the present study did not observe morphological changes of the kidney between the groups, but was observed increased gene expression of these cytokines in the renal tissue of CAF-D animals.This may have been triggered by the high presence of AGEs in plasma, which present in the bloodstream come into contact with the kidney binds to RAGE receptors, triggering inflammation in the organ.Although some studies in the literature indicate inflammation in adipose tissue as the beginning of the inflammatory process in obesity, and subsequently by the circulation affects the other organs, we do not observe it in this work, since we did not found increased IL-6 and TNF-α in adipose tissue or in plasma of the animals fed CAF-D.This leads us to emphasize that in an early period of obesity, inflammation manifests independently and by different mechanisms in different organs.Thus, we note that the oxidative stress caused by metabolic changes in obesity and hyperglycemia leads to inflammation.Obese animals showed an accumulation of AGEs in the plasma and it is clear that if this obesity persisted beyond the proposed period (6 weeks), kidney inflammation that can become a factor for progression of renal complications.TNF-α is a key mediator of inflammation and a major participant in the pathogenesis of kidney injury by promoting inflammation apoptosis and the accumulation of extracellular matrix by reducing rate flow glomerular (GFR) and increasing albumin permeability. 25ogether, the AGEs in plasma could be an biomarker for kidney disease, since studies have reported that when there is a reduction in the rate of glucose, or even decreased intake of sugars and processed food which contains large amount of AGEs, 26 improves the renal function. 27nclusIon These data allow us to conclude that a short exposure period and the cafeteria diet reflects on metabolic changes, elevated plasma levels of toxic products how AGEs, which can increased expression of inflammatory cytokines.

Figure 1 .
Figure 1.Adiposity Index in Control and Cafeteria diet animals over 6 weeks.Data are expressed as mean ± SE.Different letters indicate statistical difference between groups (t-test for independent variables p < 0.05)

Figure 2 .
Figure 2. Plasma advanced glycation end products (AGEs) in Control and Cafeteria diet animals over 6 weeks.Data are expressed as mean ± SE.Different letters indicate statistical difference between groups (t-test for independent variables p < 0.05)