African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Construction and analysis of a cDNA library from yellow-fruit ginseng (Panax ginseng C.A.Meyer.) leaf tissue

Chengjun Yang1,2, Jun Wang2,3*, Chuanping Yang2 and Guifeng Liu2
  1Postdoctoral Research Centres of Biology, Northeast Forestry University, Harbin 150040, P. R. China. 2Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, College of forestry, Northeast Forestry University, Harbin 150040, P. R. China. 3College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P. R. China.
Email: [email protected]

  •  Accepted: 09 July 2009
  •  Published: 16 November 2009

Abstract

 

The total RNA was isolated from yellow-fruit ginseng (Panax ginseng C.A. Meyer) leaf tissue. A cDNA library of panax ginseng leaves was constructed by using pDNR-LIB vector according to the SMART cDNA library construction kit protocol. We obtained 378 high quality sequences (GenBank accession number: ES672876-ES673253). ESTs were annotated, analyzed by BlastX and functional classified based on gene ontology, the results showed that 221 ESTs showed significant similarities to gene sequences in Nr database and were known genes, 21 ESTs were non-significance and unknown function genes, and 136 ESTs were considered novel genes. Most of the ESTs appeared to be related to physiological and cellular processes.

 

Key words: cDNA library, expressed sequence tags (EST), Panax ginseng.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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