African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Evaluation of genotoxicity of profenofos to freshwater fish Channa punctatus (Bloch) using the micronucleus assay

Atindra Kumar Pandey*
  • Atindra Kumar Pandey*
  • Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources, Lucknow- 226002, U.P., India; Environmental Toxicology Laboratory, Department of Zoology, University of Lucknow, Lucknow, U.P., India.
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N. S. Nagpure
  • N. S. Nagpure
  • Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources, Lucknow- 226002, U.P., India.
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Sunil P. Trivedi
  • Sunil P. Trivedi
  • Environmental Toxicology Laboratory, Department of Zoology, University of Lucknow, Lucknow, U.P., India.
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  •  Received: 09 August 2014
  •  Accepted: 22 September 2014
  •  Published: 24 September 2014

Abstract

The present study investigates the genotoxicity of profenofos (PFF), an organophosphate pesticide in erythrocyte cells of freshwater fish, Channa punctata (Bloch) using micronucleus assay. The 96 h LC50 value of PFF (50% EC) was estimated for the fish species in a semistatic system and then 50% of LC50 was determined as 1.15 ppb. The fish specimens were exposed to 1.15 ppb sublethal concentration of the pesticide and samplings were done on 24, 48, 72 and 96 h post exposure for assessment of DNA damage by micronucleus assay. The study confirms that PFF is toxic for aquatic organism and the micronucleus assay is a useful tool in determining the potential genotoxicity and mutagenicity of xenobiotic compounds and can be considered as sensitive parameter for toxicity monitoring program.

 

Key words: Genotoxicity, profenofos, Channa punctatus, DNA damage, micronucleus assay.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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