Acute Kidney Injury in Pregnancy

which is rarely seen in patients with other forms of pregnancy-related hepatic injury, including preeclampsia. The diagnosis should be suspected in a woman with preeclampsia who has hypoglycemia, hypofibrinogenemia, and a prolonged PTT in the absence of abruptio placentae, high bilirubin, normal/ high transaminases, microvesicular steatosis and rarely liver necrosis. Criteria for diagnosis of AFLP include six or more of the following features in the absence of another explanation: Vomiting, abdominal pain, polydipsia / polyuria, encephalopathy, elevated bilirubin, hypoglycemia, elevated urate, leucocytosis (>11x109/l), ascites or bright liver on ultrasound scan, elevated transaminases (aspartate aminotransferase or alanine aminotransferase >42 IU/l ), elevated ammonia, renal impairment, coagulopathy (prothrombin time >14 s or activated partial thromboplastin time >34 s), microvesicular steatosis on liver biopsy. (Ch’ng,2002). The liver biopsy is diagnostic but is not always feasible especially in patients with severe coagulopathy (Bacq, 2006) and it seldom influences acute management. Ultrasound and computed tomography have been used but false negative results are common. Renal biopsy shows Acute tubular necrosis (ATN). Delivery of the fetus, regardless of gestational age, is the only treatment for acute fatty liver of pregnancy (AFLP) once the diagnosis has been made. Treatment includes intravenous fluids, cryoprecipitate, Fresh frozen Plasma and glucose. It reverses with delivery. C section is preferred over vaginal delivery. Liver transplant is the treatment of choice in patients with liver necrosis. There is 20-25% maternal and fetal mortality and the syndrome can recur in subsequent pregnancies with a calculated genetic risk of 25%.The other causes of jaundice during pregnancy include cholestasis, cholelithiasis, viral hepatitis and pre-eclampsia with or without HELLP syndrome. Intrahepatic cholestasis of pregnancy may during the third The first section of the book covers the basics of nephrology and second section focuses on acute kidney injury. This easy to reference text examines the physiological and biochemical aspects of renal diseases - all in one convenient resource. Experts in the field discuss topics of increasing concern in nephrology including newer methods of assessing renal function. The field of acute kidney injury in nephrology is a rapidly evolving one with research translating into clinical guidelines and standards. This text brings together experts to provide an authoritative reference for management of AKI in various clinical settings. Pregnancy related AKI is an important entity which has also been discussed in detail. The recent advances in the field of critical care AKI have been incorporated as well and help the reader to update their knowledge.

have symptoms or signs like preeclampsia.It is a retrospective diagnosis and is confused with preeclampsia at the time of onset and with essential hypertension postpartum until the blood pressure returns to normal.Proteinuria does not occur, serum uric acid is normal.It may predict the development of hypertension later in life.It should be managed as preeclampsia when first diagnosed.Chronic hypertensioni s a s s o c i a t e d w i t h u n d e r l y i n g or preexisting hypertension.The diagnosis is established by a blood pressure of 140/90 mm Hg or greater before pregnancy or before 20 weeks' gestation, or by persistent hypertension after delivery.Renal biopsy shows nephrosclerosis rather than preeclampsia.Complications of chronic hypertension include superimposed preeclampsia and renal failure, abruptio placentae, growth restriction and fetal death.Methyldopa is the preferred treatment agent, but in mild cases, no therapy may be necessary.In women whose blood pressure is well controlled when they enter pregnancy, the same therapy should be continued.However, angiotensin II receptor blocking (ARB) agents and angiotensin converting enzyme inhibitors (ACEIs) are contraindicated in pregnancy due to the risk of fetal renal agenesis.Preeclampsia -It is the leading cause of acute kidney injury in the last trimester and occurs in 7% of all pregnancies mostly in primigravidas.However it can be seen in multigravidas if the interpregnancy interval is prolonged.It is the leading cause of maternal and fetal mortality in the world.It is associated with intrauterine growth retardation and small for gestational age (SGA) babies.It is a triad of hypertension, proteinuria and oedema occurring after the 20 th week of gestation with few cases developing postpartum within hours, usually in the first 24 to 48 hours.Preeclampsia does not occur before twenty weeks gestation except in molar pregnancies and in the presence of antiphospholipid antibody syndrome.Hypertension is defined as rise in systolic BP >30 mmHg and diastolic BP >15 mmHg.Proteinuria is defined as >300mg protein in urine per day.Oedema is not essential for diagnosis but sudden oedema and weight gain are common presenting features.Oedema is due to overfill with reduced GFR as in nephritic illnesses with suppressed renin and aldosterone and differs from oedema of CHF, cirrhosis and neprhotic syndrome which is associated with underfilling of vascular bed with high renin and aldosterone.Though increased capillary permeability and hypoalbuminemia may be seen they are not the sole contributing factors for oedema.The risk factors associated with the development of preeclampsia include age older than 35 years or younger than 16 years, chronic hypertension, obesity, preexisting diabetes , renal disease, insulin resistance, Anti phospholipid antibody syndrome, African American race and pregnancy with a new partner.Fetal risk factors may be twin or multiple gestations, trisomy 13 and hydatidiform mole.Some genetic factors contribute to the development of preecclampsia.It is four times more common if there is history of precclampsia in mother or sister.Polymorphisms of genes associated with control of blood pressure or coagulation system have been implicated.Some mutations described involve Endothelial nitric oxide synthetase (e NOS), renin angiotensinogen (T235), Leyden factor V, Prothrombin and Methyl tetra hydrofolate reductase(MTHFR) genes.A locus on 2p was described in Icelandic and Australian families.A Dutch study reported linkage to 12 q locus in HELLP syndrome which shows the genetic factors responsible for Preclampsia and HELLP may be different.Association with trisomy 13 indicates a linkage to chromosome 13.An activating mutation of mineralocorticoid receptor was described in patients with PIH but no proteinuria.Preclampsia is more commonly seen in women at high altitude or in third world countries suggesting the possible role of hypoxia or environmental pollution in genesis .HELLP syndrome (Hemolysis, ELevated liver enzymes, and Low Platelets) is observed when severe preeclampsia or eclampsia is accompanied by significant liver involvement.Acute renal failure (ARF) may develop.(Lindheimer, 1991) Eclampsia-is the occurrence of seizure activity with no other explainable cause in setting of preeclampsia.Preeclampsia superimposed on chronic hypertensiondefined as new-onset proteinuria (ie, >300 mg/d) after 20 weeks' gestation in a hypertensive patient or as a sudden increase in proteinuria or blood pressure in a patient with hypertension and proteinuria before 20 weeks' gestation.

Pathophysiology
Utero-placental ischemia: A combination of genetic and environmental factors results in inadequate invasion of uterine spiral arteries by placental trophoblasts resulting in inability of uterine vessels to transform from high resistance channels to a low resistance system.This results in utero-placental ischemia.In normal pregnancy Vascular endothelial growth factor (VEGF ) and Placental induced growth factor (PIGF) are made by placenta and circulate in high concentrations in the blood.VEGF is also synthesized by glomerular podocytes and vascular endothelial cells.VEGF and PIGF induce synthesis of nitric oxide and vasodilating prostacylin in endothelial cells decreasing vascular tone and blood pressure.In PIH the utero-placental ischemia results in oxidative stress and release of a soluble cytokine, the sFlt-1 (soluble fms-like tyrosine kinase-1).sFlt-1 is a potent antagonist of VEGF and PIGF.In PET concentrations of sFlt-1 rise in the 2 nd trimester with a decrease in VEGF and PIGF.Overproduction of sFlt-1 explains increased susceptibility to PET in multiple gestation, hydantidiform mole, trisomy 13 and first pregnancy.In addition, increased concentration of circulating pre-ecclamptic factorsangiotensin 1 /Bradykinin B2 receptor heterodimers, agonistic antibodies to angiotensin I receptor(AT 1 receptor antibody) and soluble endoglin are seen in PET.(Maynard,S 2003;Venkatesha,S 2006;Zhou,C.C,2008;Levine, RJ 2006) There is an imbalance of vasoconstrictors and vasodilator substances hence there is a state of generalized vasoconstriction in preeclampsia.The levels of prorenin, angiotensin, thromboxane and endothelin are high while the levels of vasodilator prostaglandin ie Prostacyclin, PGE2, nitric oxide and bradykinin are low.In normal pregnancy there is less sensitivity of vascular bed to the hypertensive effects of angiotensin but this is lost in preeclampsia.A myriad of markers for generalized endothelial dysfunction are seen in preeclampsia which include von Willebrand factor, Platelet activating factor (PAF-1), Endothelin and cellular fibronectin.The increase in blood volume, increased GFR and vasodilatation seen in normal pregnancy are lost in preeclampsia.Though the blood volume in preeclampsia is decreased there is an increase in effective circulating volume with suppressed renin and aldosterone with elevation of brain natriuretic peptide.

Screening tests
Laboratory evaluation helps to determine disease severity by characterizing the extent of end organ involvement.Hematocrit -Hemoconcentration supports the diagnosis of preeclampsia but hemolysis, if present, can decrease the hematocrit.
Platelet count -Preeclampsia accounts for 21% of cases of maternal thrombocytopenia.Thrombocytopenia is usually moderate and platelet count rarely decreases to < 20,000/μL.Thrombocytopenia in patients with preeclampsia always correlates with the severity of the disease.It is considered a sign of worsening disease and is an indication for delivery.Quantification of protein excretion -Excretion of 300 mg or more in 24 hours is necessary for diagnosis.It is suggested by at least 1+ protein on dipstick of two urine specimens collected at least four hours apart.A dipstick of 3+ or greater or 5 g or more per day is a criterion of severe disease.Serum creatinine concentration -An elevated or rising level suggests severe disease.Serum alanine and aspartate aminotransferase concentrations (ALT and AST) -Elevated or rising levels suggest hepatic dysfunction indicative of severe disease.Serum lactate dehydrogenase (LDH) concentration -Microangiopathic hemolysis is suggested by an elevated LDH level and red cell fragmentation (schistocytes or helmet cells) on peripheral blood smear.Elevation of total bilirubin may also suggest hemolysis.Microangiopathic hemolysis is present in severe disease or HELLP syndrome (Hemolysis, Elevated Liver function tests, Low Platelets).Serum uric acid concentration -It is often elevated in preeclampsia, but not diagnostic.High uric acid levels may be due to hypoxia or due to reduced GFR.Fetal well-being is evaluated by a nonstress test or biophysical profile.In addition, the fetus is examined by ultrasound to evaluate growth and amniotic fluid volume.Assessment of umbilical artery Doppler flow may also aid in evaluation of the fetal condition.Coagulation function tests (eg, prothrombin time, activated partial thromboplastin time, fibrinogen concentration) are usually normal if there is no thrombocytopenia or liver dysfunction, and therefore do not need to be monitored routinely.Recently, urinary PlGF values and sFlt-1 values have shown great promise as a means of identifying preclinical preeclampsia .

Management
The definitive treatment of preeclampsia is delivery to prevent development of maternal or fetal complications from disease progression.Several groups have formulated guidelines for diagnosis, evaluation, and management of hypertensive disorders of pregnancy (NHBPEP.Report of the National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy (2000), ACOG 2002).Laboratory follow-up -The minimum laboratory evaluation should include platelet count, serum creatinine, and serum AST.These tests should be repeated once or twice weekly in women with mild preeclampsia to assess for disease progression, and more often if clinical signs and symptoms suggest worsening disease (ACOG 2000).The value of other tests is less clearly defined.A rising hematocrit can be useful to look for hemoconcentration, which suggests contraction of intravascular volume and progression to more severe disease, while a falling hematocrit may be a sign of hemolysis.An elevated serum LDH concentration is also a sign of hemolysis, and a marker of severe disease or HELLP syndrome.Hemolysis can be confirmed by observation of schistocytes and helmet cells on a blood smear .Quantification of protein excretion can be performed to determine whether the threshold for severe preeclampsia (5 g/24 hours) has been reached.Since several clinical studies have shown that neither the rate of increase nor the amount of proteinuria affects maternal or perinatal outcome in the setting of preeclampsia, repeated 24-hour urinary protein estimations are not useful once the threshold of 300 mg/24 hours for the diagnosis of preeclampsia has been exceeded.Serum creatinine alone can be used to monitor renal function.
Treatment of hypertension -Nonpharmacologic treatments include bed rest and alcohol avoidance.Weight loss and salt restriction are not recommended.ACE inhibitors are contraindicated.Diuretics in pregnancy lead to intravascular volume depletion, organ and placental hypoperfusion.They may be used with caution in presence of significant edema.If preeclampsia develops diuretics must be discontinued.Beta-blockers have no major contraindications, although neonatal bradycardia, hypoglycemia, and respiratory depression can occur.Labetalol is not associated with neonatal bradycardia and is used for hypertensive emergencies.Alpha-methyldopa is the drug of choice for hypertension.Clonidine is also used.Calcium channel blockers may cause tocolysis in 3 rd trimester hence should be used only if hypertension is unresponsive to other medications.Hydralazine is a first-line agent for hypertensive emergencies.Experience with minoxidil and prazosin is limited.The use of antihypertensive drugs to control mildly elevated blood pressure in the setting of preeclampsia does not alter the course of the disease or diminish perinatal morbidity or mortality.Novel therapies are under investigation.L-arginine is the physiologic precursor for nitric oxide, which has been implicated in the pathogenesis of preeclampsia and is being tested.Another randomized trial showed that plasma volume expansion did not improve maternal or fetal outcome.Assessment of fetal well-being -A minimum of daily fetal movement counts and twice weekly fetal non stress testing with assessment of amniotic fluid volume, or biophysical profile is recommended.Early fetal growth restriction may be the first manifestation of preeclampsia or a sign of severe preeclampsia.Sonographic estimation of fetal weight should be performed to look for growth restriction and oligohydramnios at the time of diagnosis of preeclampsia and then repeated serially.Doppler velocimetry is useful for assessing fetal status if fetal growth restriction is present.Delivery-The decision to deliver the fetus is based upon gestational age, maternal and fetal condition, and the severity of preeclampsia.Mild PIH-Patients at term (>37 weeks) are delivered.Most experts advise that delivery should not be postponed beyond 40 weeks of gestation in any preeclamptic woman.Vaginal delivery is preferred at term if there are no contraindications.Cervical ripening agents should be considered in women with unfavorable cervices.Women with mild PIH with period of gestation <37 weeks can be managed expectantly.However maternal end-organ dysfunction and non reassuring tests of fetal well-being may be indications for delivery at any gestational age.Outpatient care is a cost-effective option for some women with mild preeclampsia.However these patients should comply with frequent maternal and fetal evaluations (every one to three days) and have ready access to medical care.Restricted activity is recommended; however, complete bed rest is not required.Hospitalization is needed in the event of fulminant progression to eclampsia, hypertensive crisis, abruptio placentae, or HELLP syndrome.Patients should be hospitalized immediately if they develop severe or persistent headache, visual changes, right upper quadrant or epigastric pain, nausea or vomiting, shortness of breath, or decreased urine output (ACOG 2000).As with any pregnancy, decreased fetal movement, vaginal bleeding, abdominal pain, rupture of membranes, or uterine contractions should be reported immediately.Severe preeclampsia -Severe preeclampsia is generally regarded as an indication for delivery, regardless of gestational age, to minimize the risk of development of maternal and fetal complications.Prolonged antepartum management at a tertiary care setting or in consultation with a maternal-fetal medicine specialist may be considered in few women under 32 to 34 weeks of gestation.However, women who develop severe preeclampsia at or beyond 32 to 34 weeks of gestation should be delivered at an institution with appropriate facilities.Hyaline membrane disease is common in preterm infants of preeclamptic women.Therefore, antenatal corticosteroids (betamethasone) to promote fetal lung maturity should be administered to women less than 34 weeks of gestation since preterm delivery is common.Even in preterm delivery vaginal delivery may be attempted.Cervical ripening agents may be used prior to induction if the cervix is not favorable.However, a prolonged induction should be avoided.Some authors recommend cesarean delivery for women with severe preeclampsia who are under 30 weeks of gestation .Management of severe preeclampsia-or eclampsia-related pulmonary edema includes treatment of the severe preeclampsia or eclampsia, supplemental oxygen, and fluid restriction.Diuresis is indicated if there is fluid overload but care should be taken to avoid further intravascular volume depletion.Mechanical ventilation may be necessary.Close, continuous maternal-fetal monitoring is indicated intrapartum to identify worsening hypertension, deteriorating maternal hepatic, renal, cardiopulmonary, or hematologic function, and uteroplacental insufficiency or abruptio placentae (often manifested by fetal bradycardia/or vaginal bleeding).The vitals should be monitored every hour and preeclampsia laboratory tests should be repeated every six hours.Epidural anaesthesia should be avoided in presence of thrombocytopenia.Invasive hemodynamic monitoring can be useful in complicated patients.Anticonvulsant therapy is generally initiated during labor or while administering corticosteroids or prostaglandins prior to planned delivery.Therapy is continued for 24 hours postpartum (range 12 to 48 hours).Magnesium sulfate is the drug of choice for the prevention of eclampsia and prevention of recurrent eclamptic seizures.It is more effective than Phenytoin or Nimodipine or lytic cocktail ( mixture of chlorpromazine, promethazine and pethidine) .Magnesium causes vasodilatation of the cerebral vasculature, inhibition of platelet aggregation, protection of endothelial cells from damage by free radicals, prevention of calcium ion entry into ischemic cells, decreasing the release of acetylcholine at motor end plates within the neuromuscular junction, and as a competitive antagonist to the glutamate N-methyl-D-aspartate receptor (which is epileptogenic).Anticonvulsant therapy is also used for prevention of seizures in women with mild preeclampsia, but its role in this setting is controversial.The magnesium sulfate dose is 4 g intravenously as a loading dose then 1 g/h, or 5 g intramuscularly into each buttock followed by 5 g intramuscularly every four hours.Hypotension is the major concern from regional anesthesia since preeclamptic women have depleted intravascular volumes.Outcome -The major adverse outcomes associated with preeclampsia are related to maternal central nervous system, hepatic, and renal dysfunction (eg, cerebral hemorrhage, hepatic rupture, renal failure), bleeding related to thrombocytopenia, preterm delivery, fetal growth restriction, abruptio placentae, and perinatal death.Factors that influence outcome include gestational age at onset and delivery, severity of disease, and whether there are coexisting conditions present, such as multiple gestation, diabetes mellitus, renal disease, thrombophilia, or preexisting hypertension.There is approximately one maternal death due to preeclampsia-eclampsia per 100,000 live births, with a case-fatality rate of 6.4 deaths per 10,000 cases in US.In mild preeclampsia neonatal outcomes are generally good and comparable to those of normotensive women.Risk of recurrence in subsequent pregnancy depends upon severity of disease, gestational age at onset and gestational age at delivery.Postpartum course -Vital signs are monitored every two hours while the patient remains on magnesium sulphate and laboratory tests are repeated until two consecutive sets of data are normal.Hypertension and proteinuria due to preeclampsia resolve postpartum, often within a few days, but sometimes take few weeks or rarely a year or more.Antihypertensive medications can be discontinued when blood pressure returns to normal levels.Elevated blood pressures that remain 12 weeks postpartum are unlikely to be related to preeclampsia and may require long-term treatment.Vasoconstriction and endothelial dysfunction resolve over a few days.Postpartum preeclampsia Pre eclampsia /eclampsia occurring more than two days and less than six weeks after delivery.It is important to exclude other diagnoses, especially when symptoms suggest the possibility of central nervous system pathology.Late postpartum eclampsia is defined as eclamptic seizures developing greater than 48 hours, but less than four weeks postpartum).Controversial prevention strategies include calcium supplementation, fish oils, sodium restriction, low-dose aspirin and antioxidants.

Hemolysis, elevated liver enzymes, low platelets -HELLP syndrome
HELLP syndrome is a variant of severe preeclampsia, first described by Dr. Louis Weinstein in 1982.About 15 to 20 percent of affected patients do not have antecedent hypertension or proteinuria, leading some experts to believe that HELLP is a separate disorder from preeclampsia [Sibai BM1986, Reubinoff BE, 1991].Both severe preeclampsia and HELLP syndrome may be associated with other hepatic manifestations, including infarction, hemorrhage, and rupture.HELLP develops in approximately 1 to 2 per 1000 pregnancies overall and in 10 to 20 percent of women with severe preeclampsia/ eclampsia.The majority of cases are diagnosed between 28 and 36 weeks of gestation with 70 percent occurring prior to delivery [Sibai BM, Ramadan MK 1993].Of these patients, approximately 80 percent are diagnosed prior to 37 weeks of gestation and fewer than 3 percent develop the disease between 17 and 20 weeks of gestation.The disease presents postpartum in 30 percent, usually within 48 hours of delivery, but occasionally as long as seven days after birth.Only 20 percent of postpartum patients with HELLP have evidence of preeclampsia antepartum.

Diagnosis and classification of HELLP syndrome
There is no consensus regarding the degree of laboratory abnormality diagnostic of HELLP syndrome.Some studies use AST (and/or ALT) and LDH values above the upper limit of normal, while others require elevations of at least two standard deviations above the mean.Due to differences in assays used to measure these enzymes, an elevated value in one hospital may be near normal in another.HELLP syndrome and severe preeclampsia are probably part of a disease spectrum.A precise definition of HELLP is necessary for research purposes and for predicting maternal complications.We require the presence of all of the following criteria to diagnose HELLP.Microangiopathic hemolytic anemia with characteristic schistocytes (also called helmet cells) on blood smear.Other signs suggestive of hemolysis include an elevated LDH or indirect bilirubin and a low serum haptoglobin concentration (≤25 mg/dL).Platelet count ≤100,000 cells/microL .HELLP syndrome accounts for 21% of maternal thrombocytopenia in pregnancy.Serum LDH ≥600 IU/L or total bilirubin ≥1.2 mg/dL Serum AST ≥70 IU/L.Some investigators obtain ALT levels instead of, or in addition to, AST levels.An advantage of the AST is that it is a single test that reflects both hepatocellular necrosis and red cell hemolysis.Women who do not meet all of the above laboratory abnormalities are considered to have partial HELLP syndrome.These patients may progress to complete expression of HELLP syndrome.Approximately 50% of patients have complete HELLP (all components present), and 50% have incomplete HELLP (at least 1 components present: EL, HEL, ELLP, LP).Some physicians subclassify HELLP based on the severity of thrombocytopenia, as follows: Class 1 -Platelet count < 50,000/μL, Class 2 -Platelet count 50,000-100,000/μL, Class 3 -Platelet count 100,000-150,000/μL

Clinical manifestations
Clinical manifestations are nonspecific (nausea, vomiting, headache in 50%, epigastric or right upper quadrant pain in 50%).Early HELLP syndrome is often misdiagnosed as heartburn, hence a high index of suspicion for HELLP syndrome is important.All patients with HELLP syndrome do not meet the strict criteria for preeclampsia.Hypertension (blood pressure ≥140/90) and proteinuria are present in approximately 85 %, 15% have diastolic blood pressure (BP) >90 mm Hg but either or both may be absent in women with severe HELLP syndrome (Sibai, 2004).Some patients are asymptomatic, major complications can occur despite normal blood pressure and proteinuria.These include DIC, abruptio placentae, acute renal failure, pulmonary edema, subcapsular liver hematoma, retinal detachment or stroke.Jaundice and ascites may also be present.Bleeding may occur due to thrombocytopenia.Thrombocytopenia is usually moderate, with counts rarely <20,000/μL.Major hemorrhage is uncommon.Maternal thrombocytopenia reaches a nadir at 24-48 hours postpartum.The maternal mortality rate with HELLP is 1%.The perinatal mortality rate is 11%.Fetal growth restriction is common.Neonates may be at increased risk for thrombocytopenia.

Treatment
Management is like preecclapsia .Delivery is the ultimate cure and should be planned immediately if >34 weeks or can be delayed for 24-48 hours if <34 weeks' gestation to administer corticosteroids if the patient is asymptomatic and the fetal testing is normal.Magnesium sulfate (MgSO 4 ) should be administered intrapartum and postpartum, regardless of blood pressure levels, to prevent seizures (eclampsia).Approximately 6-10 units of platelets is administered at the time of skin incision, and an additional 6 units are administered if oozing is noted during the surgery.Also platelet transfusion is indicated if the platelet count is less than 20,000 cells/uL.If cesarean delivery is planned platelet transfusion, is recommended to achieve platelet count greater than 50,000 cells/uL.Thrombocytopenia and elevated liver function tests worsen postpartum but should start normalizing by the third postpartum day.The use of steroids for HELLP is controversial.Management of hepatic complications -Marked elevations in serum aminotransferases are not typical of uncomplicated HELLP; when they occur, the possibility of hepatic infarction, subcapsular hematoma, or viral hepatitis should be considered.Marked elevation in serum aminotransferases (usually 1000 to 2000 IU/L or higher) associated with right upper quadrant pain and fever is characteristic of hepatic infarction; this diagnosis can be confirmed by hepatic imaging.These infarcts resolve post partum.These patients may have an underlying procoagulant state, such as the antiphospholipid syndrome.HELLP may be complicated by hepatic rupture with development of a hematoma beneath Glisson's capsule.Histology of the liver adjacent to the rupture shows periportal hemorrhage and fibrin deposition, along with a neutrophilic infiltrate( hepatic preeclampsia).The hematoma may remain enclosed, or rupture, with hemoperitoneum .A hepatic hematoma rarely occurs in the absence of preeclampsia or HELLP .It is characterized by abdominal pain and many have severe thrombocytopenia, shoulder pain, nausea, and vomiting.If hepatic rupture occurs, swelling of the abdomen from hemoperitoneum and shock is seen.The aminotransferases are elevated, and values of 4000 to 5000 IU/L can occasionally be seen.Imaging using CT or MR is more sensitive than ultrasonography for these lesions.The management of a contained hematoma is to support the patient with volume replacement and blood transfusion, as needed, with consideration of percutaneous embolization of the hepatic arteries.It takes months for the hematoma to resolve completely.Surgical intervention is indicated if there is hemodynamic instability, persistent bleeding, increasing pain, or continued expansion of the hematoma.Operative management includes packing, drainage, hepatic artery ligation, and/or resection of affected areas of the liver.For patients with intractable hemorrhage, administration of recombinant factor VIIa and liver transplantation are recommended.Patients who survive have no hepatic sequelae.Postpartum couse -Decreasing platelet counts continue until 24 to 48 hours after delivery, while serum LDH concentration usually peaks 24 to 48 hours postpartum.An upward trend in platelet count and a downward trend in LDH concentration should be seen by the fourth postpartum day in the absence of complications.Recovery can be delayed in women with DIC, platelet count less than 20,000 cells/uL, renal dysfunction or ascites.Outcome is generally good; however, serious complications are relatively common.In a series of 437 women with HELLP syndrome at a tertiary care facility, the following complications were observed DIC -21 %, Abruptio placentae -16 %, Acute renal failure -8 %, Pulmonary edema -6 %, Subcapsular liver hematoma -1%, Retinal detachment -1 %.In addition, 55 % of the patients required blood or blood products, and 2 % required laparotomies for intraabdominal bleeding.1 % died.Other complications include: adult respiratory distress syndrome, sepsis, and stroke.Wound complications secondary to bleeding and hematomas are common in women with thrombocytopenia.Fetal complications include prematurity (70 %), intrauterine growth restriction and abruptio placenta, and depend largely upon the severity of the disease and the gestational stage.The overall perinatal mortality is 7 to 20 %.However, surviving babies do not have an increased risk of liver disease or thrombocytopenia.Although most liver function tests return to normal postpartum , rarely high total bilirubin may be seen in 20 % even till 3 years post partum.HELLP syndrome with or without renal failure does not affect longterm renal function.The rate of recurrence is 2 -27 %.Recurrent hepatic rupture in a subsequent pregnancy have been reported, suggesting there may be a genetic predisposition to this condition.Women with a history of HELLP syndrome are at high risk for developing preeclampsia in a subsequent pregnancy.The incidence of preeclampsia varies from 20-50 percent in normotensive women to 75 percent in those with underlying hypertension.There is no evidence that any therapy prevents recurrent HELLP syndrome.

Microangiopathies
Microangiopahy is seen in pre eclampsia.Two other important conditions where microangiopathy in pregnancy is seen are Thrombotic thrombocytopenic purpura (TTP) and hemolytic uremic syndrome (HUS) which are characterized by thrombocytopenia, hemolytic anemia, and multiorgan failure.Incidence is 1 in 25,000 births.TTP is known for central nervous system involvement, while HUS predominantly affects the kidneys.Significant overlap exists in the clinical manifestations of TTP and HUS.Pathogenesis of microangiopathies -Normally the endothelium is smooth and platelets do not adhere to the endothelium.In microangiopathies the endothelium is damaged and there is formation of thrombi.Complement activation plays an important role in endothelial damage.Role of complement-Complement is a system of serum proteins.The complement cascade is activated through three pathways (i.e., classical, lectin, and alternative pathways) that converge in the generation of C3 convertase.Because the alternative pathway is initiated spontaneously, the alternative C3 convertase (C3bBb) is tightly regulated by plasma and membrane-bound factors, mainly factor H (FH), factor I(FI), membrane cofactor protein (MCP), and decay-accelerating factor (DAF).Complement activation leads to endothelial damage.Role of platelets-Platelets are nonnucleated cells derived from megakaryocytes in the bone marrow and normally live in the peripheral circulation for as long as 10 days.Platelets play a critical initiating role in the hemostatic system.Primary hemostasis begins when platelets adhere to the site of endothelial disruption, leading to platelet clumping.This is followed by platelet activation, characterized by release of granules containing von Willebrand factor, adenosine 5'-diphosphate (ADP), and serotonin.This attracts other platelets into the platelet plug, which stops the bleeding.Simultaneously, the synthesis of thromboxane A 2 and release of serotonin leads to vasoconstriction to reduce blood loss at the site of vascular injury.The secondary hemostatic phase begins when the coagulation pathway is activated on the surface of the activated platelets to form a secondary thrombus with fibrin.Abnormal intravascular platelet aggregation leads to microthrombi formation, which results in thrombocytopenia, intravascular hemolysis from the breakage of red blood cells through partially occluded vessels, and end organ ischemia.Genesis of thrombocytopenia-The platelet count in nonpregnant women is 150,000-400,000/μ L. Average platelet count in pregnancy is decreased (213,000/μ L vs 250,000/μ L).Low platelet count is due to hemodilution, increased platelet consumption, and increased platelet aggregation driven by increased levels of thomboxane A 2 .Thrombocytopenia can be defined as platelet count less than 150,000/uL or platelet count below the 25 th percentile for pregnant patients (116,000/μ L).In pregnancy mild,moderate and severe thrombocytopenia are defined as platelet count of 100,000-150,000/μL; 50,000-100,000/μ L and <50,000/μ L respectively.Development of TMA-An acquired or constitutional deficiency in ADAMTS13, a von Willebrand factor (vWF)-processing enzyme, is an important cause of of a peculiar type of TMA.ADAMTS13 levels fall during the last two trimesters of pregnancy, hence TTP is more common at this time.(Fadi Fakhouri, 2010).Development of HUS-The pathogenesis of pregnancy-associated atypical hemolytic uremic syndrome (P-aHUS) remains obscure and viral illness, retained placental fragments, drugs eg oxytocics, ergot and oral contraceptives have been implicated.The severe deficiency in the von Willebrand factor cleaving protease (ADAMTS13) seen in TTP is rarely present in patients with HUS.P-aHUS may be related to alternative C3 convertase dysregulation.A lack of control of the alternative C3 convertase leads to complement-induced lesions of the endothelial cells.Acquired (anti-FH antibodies) or constitutional (inactivating mutations in FH, FI, or MCP coding genes or activating mutations in factor B and C3 coding genes) with dysregulation of the alternative C3 convertase has been established as a risk factor for the occurrence of aHUS.It is assumed that excessive activation of the alternative C3 convertase leads to complement activation.Pregnancy may trigger the onset or subsequent relapses.Mutations in the SCR19-20 domains of factor H are less frequent in P-aHUS patients compared with non-pregnancy-related aHUS.

Hemolytic uremic syndrome(HUS)/Idiopathic post partum failure
It occurs in primipara and is characterized by renal failure, anemia and hypertension.The risk for P-aHUS is highest during a second pregnancy.Onset is within hours to days post partum.Symptoms can begin before delivery, but the onset in most cases is delayed for 48 hours or more after delivery (mean four weeks).HUS may follow a normal pregnancy or be preceded by findings indistinguishable from preeclampsia.HUS with severe renal failure more frequently presents in the postpartum period.Presenting symptoms are often nonspecific , although majority (60%) present with bleeding.Hypertension is seen in 75% of cases.Hemolysis and anemia may be absent at presentation in 50% of cases.DIC is rare.Labs -Obligate findings include hemolytic anemia (hematocrit < 30% with schistocytes on peripheral smear) and thrombocytopenia under 100,000/μ L (50% of patients will have counts < 20,000/μ L).LDHis usually >600 U/liter.Hemolytic anemia is coomb's negative.There may be hypocomplementemia, deficiency in prostaglandins or antithrombin levels.HUS is a clinical diagnosis.Tissue biopsy is not required.In problematic cases when renal biopsy is done it shows mesangiolysis and glomerular simplification.Outcomes-The outcomes are poor.80-90% survive acute episode.There is a high maternal mortality of 18-44% and fetal loss is seen in 80%.62% reached ESRD by 1 month.Outcomes do not differ between patients with pregnancy-related and non-pregnancy-related aHUS.Pregnancies in female patients with complement abnormalities are complicated by fetal loss and preeclampsia in 4.8% and 7.7%,respectively.(Fadi Fakhouri,2010) Almost half have residual neurologic or chronic renal failure.Recurrences occur in 50%.Long-term sequelae, such as hypertension and chronic renal failure, are observed in 44% of patients with HUS.The perinatal mortality rate is as high as 30%.Treatment includes Plasmapheresis.Plasma infusion alone is less effective.Steroids, antiplatelet therapy, Immunoabsorption, splenectomy, IV gamma globulin therapy have been used in different studies.Renal failure is managed by hemodialysis.Antihypertensives are used for control of blood pressure.Platelet transfusions should be avoided.Heparin and fibrinolytic agents and anti thrombin III concentrates may be used.Dilatation and curettage should be considered when the disease occurs very close to delivery.

Thrombotic thrombocytopenic purpura
TTP is characterized by the pentad of microangiopathic hemolytic anemia, thrombocytopenia, renal insufficiency, fever, and neurologic abnormalities.TTP usually occurs antepartum , about 12 % in the first trimester, 56 % in the second trimester, and 33 % in the third trimester/postpartum (Egerman RS, 1996), There may be mild renal failure and severe neurological involvement (headache, altered consciousness, seizures, hemiparesis), and fever.Severe thrombocytopenia and hemolytic anemia may be seen.Examination shows petechiae, ecchymoses, and nose and gum bleeding .Rarely hematuria, gastrointestinal bleeding and intracranial bleeding may occur.Bleeding associated with surgery is uncommon unless the platelet counts are lower than 50,000/μ L. Clinically significant spontaneous bleeding is rare unless counts fall below 10,000/μ L.

Treatment
Plasmapheresis is the recommended therapy.Plasmapheresis removes platelet-aggregating substances causing TTP.Treatment is 90% successful with TTP but is less successful with HUS.Steroids are used along with plasmapheresis.However, steroids are less effective than plasmapheresis (25% response rate).Platelet transfusions should be avoided when possible because they can cause a clinical deterioration.Platelet transfusions should be used only for uncontrolled bleeding or intracranial hemorrhage.Other therapies include immunosuppressive agents (vincristine, azathioprine, cyclosporine)and splenectomy for TTP.Premature termination of pregnancy has been associated with relapse.Delivery should be considered only when no response to other therapies occurs.(George JN, 2010)

Differential diagnosis
TTP, HUS and preeclampsia need to be differentiated as prognosis and treatment vary.The distinction between HUS-TTP and severe preeclampsia is often difficult.TTP is characterized by severe thrombocytopenia, hemolytic anemia, neurologic abnormalities (headache, altered consciousness, seizures, hemiparesis), and fever.Renal involvement may occur in 80% of cases of TTP; TTP occurs in 2 nd /3 rd trimester .von Willebrand factorcleaving protease (ADAMTS-13) activity <5 percent is seen in 33 to 100 percent of women with TTP.Unlike HELLP the condition may continue or worsen after delivery with TTP.If suspected preeclampsia/HELLP does not improve within 48-72 hours after delivery, consider TTP.TTP like HUS is associated with isolated platelet consumption; thus, although thrombocytopenia is seen, the other findings of DIC are typically absent.A peripheral blood smear can also be useful.The percentage of schistocytes on peripheral smear is higher in TTP (2 to 5 percent).HUS is characterized by thrombocytopenia, hemolytic anemia, acute renal failure with proteinuria, hematuria, or oliguria/anuria).Neurological manifestations are uncommon.The absence of DIC, onset more than two days after delivery, and/or persistent disease for more than one week are the main findings that characterize HUS.Neurologic involvement may be seen in 50% of cases of HUS.A peripheral blood smear can also be useful.The percentage of schistocytes on peripheral smear is higher in HUS (2 to 5 percent).ADAMTS deficiency is rare in HUS.History of preceding proteinuria and hypertension favor preeclampsia , preeclampsia-HELLP does not occur before 20 weeks of gestation and most cases are diagnosed in the third trimester.Delivery leads to resolution with preeclampsia, HELLP is associated with thrombocytopenia, and in severe cases, there may be DIC with prolongation of the PT and aPTT, and reductions in the plasma concentrations of factors V and VIII.The percentage of schistocytes on peripheral smear is low (<1%).ADAMTS deficiency is not seen in PIH.In addition, only a few select laboratories are able to perform complementassociated mutational analysis.Studies of larger heterogeneous populations need to be performed before recommending genetic analysis for patients with pregnancy-associated TTP-HUS.It has not been shown that distinguishing TTP from HUS alters treatment of such patients.

Antepartum and post partum hemorrhage
Significant blood loss due to antepartum or postpartum hemorrhage may cause ischemic injury and lead to obstetric acute renal failure.Antepartum hemorrhage may be due to placenta praevia or concealed hemorrhage due to abruptio placentae which is usually associated with PIH.Treatment consists of intravascular volume repletion and blood transfusions.If the acute renal failure is due to pre renal intravascular volume depletion and is corrected rapidly the patient may recover without dialysis.However if the insult is prolonged it may lead to acute tubular necrosis for which dialysis is needed.

Puerperal sepsis
It is an important cause of AKI.According to World Health Organization puerperal sepsis is defined as infection of the genital tract occurring at any time between the rupture of membranes or labor and the 42 day post partum in which 2 or more of the following are present: pelvic pains, fever oral temperature 38,5°C or higher on any occasion, abnormal vaginal discharge (example presence of pus), abnormal smell or foul odour of discharge, delayed uterine involution.The causative organisms are gram positive streptococcus pyogenes, staphylococcus aureus, coliforms,Chlamydia and Clostridium tetani.(Momoh ,2010)Causes are prolonged rupture of membranes, obstructed labour, frequent vaginal examinations, anemia, caesarean section etc. .The source of infection may be from the retained products of conception where there may be a history of foul smelling lochia and the retained products can be demonstrated on imaging.High vaginal swab culture may help to identify the causative organism.Other sources may be urinary tract infection or mastitis.Hence a detailed physical examination including the breast examination should be undertaken.Treatment consists of appropriate antibiotics according to the culture sensitivity reports.Surgery to remove the retained products or abscess drainage even hysterectomy in extreme cases may be needed.

Urinary Tract Infections (UTI)
Another important cause of renal failure in pregnancy is UTI.UTIs are the most common renal disease occurring during pregnancy and range from asymptomatic bacteriuria to pyelonephritis.UTIs have been associated with SGA babies, premature labor, IUD, anemia and hypertension in mother.In some cases upper UTI may be associated with renal failure.Pregnant females are at risk for development of UTIs (2-10%), because of anatomic and physiologic changes that occur in normal pregnancy.UTI may be classified as:

Asymptomatic bacteriuria
Bacteriuria occurs in 2 to 7 % of pregnancies, particularly in multiparous women, a similar prevalence as seen in nonpregnant women.A clean-voided specimen containing more than 1 lakh organisms per milliliter suggests infection.Bacteriuria often develops in the first month of pregnancy and is frequently associated with a reduction in concentrating ability.30% of patients develop pyelonephritis if asymptomatic bacteriuria is left untreated.Universal screening is therefore recommended in all pregnant females.Screening for asymptomatic bacteriuria should be performed at 12 to 16 weeks gestation (or the first prenatal visit, if that occurs later).Rescreening is generally not performed in low risk women, but can be considered in women at high risk for infection (eg, presence of urinary tract anomalies, hemoglobin S, or preterm labor).Dipstick has a sensitivity of <50% and routine microscopy and culture are needed.Urinalysis with culture should be performed on a monthly basis after resolution.Treatment with a course of oral antibiotics (Nitrofurantoin 100 mg orally every 12 hours for five to seven days, Ampicillin ,Amoxicillin (500 mg orally every 12 hours for three to seven days), Amoxicillinclavulanate (500 mg orally every 12 hours for three to seven days),Cephalexin (500 mg orally every 12 hours for three to seven days) and Fosfomycin (3 g orally as a single dose)and sulphonamides are some of the safe regimes.Treatment reduces incidence of pyelonephritis to 3%.Fluoroquinolones should be avoided in pregnancy.The safest course is to avoid using nitrofurantoin in the first trimester if another antibiotic that is safe and effective is available.Sulfonamides should be avoided in the last days before delivery because they can increase the level of unbound bilirubin in the neonate.Trimethoprim (FDA category C) is generally avoided in the first trimester because it is a folic acid antagonist, has caused abnormal embryo development in experimental animals, and some case control studies have reported a possible association with a variety of birth defects.Ceftrioxone should be avoided the day before parturition because it displaces bilirubin and may cause kernicterus.Recurrence occurs in 35%.If bacteriuria is persistent, suppressive therapy (Nitrofurantoin 50-100 mg at night , cephalexin 250-500 mg or amoxycillin) is indicated.

Cystitis
Cystitis (3%) is associated with dysuria, urgency and frequency, without systemic signs.It usually occurs in 2 nd trimester and is common even in patients with negative urine cultures.Though cystitis recurs in 17% it does not progress to acute pyelonephritis.It should be aggressively treated with oral antibiotic regimens.The symptoms of cystitis and pyuria accompanied by a "sterile" urine culture finding may be due to Chlamydia trachomatis urethritis.There may be mucopurulent cervicitis, hence erythromycin therapy is effective.

Pyelonephritis-(3%)
Majority (70%) patients with PN have asymptomatic bacteriuria.50% cases occur in 2 nd trimester.Onset is abrupt with fever, chills, flank pain, anorexia, nausea, vomiting and costovertebral tenderness.The etiologic organisms include Escherichia coli, Klebsiella, Enterobacter and Proteus .10% are due to Gram+ organsisms.15% patients have concurrent bacteremia.Other complications include hemolysis, sepsis, adult respiratory distress syndrome, hepatic dysfunction and death.Pyelonephritis requires hospitalization and intravenous antibiotics and fluids till fever resolves.Effective regimens include ampicillin plus gentamicin or a third-generation cephalosporin followed by oral administration of antibiotics for 14 days .This leads to complete resolution of infection in 70%.Antibiotics can be further adjusted according to culture sensitivity results.In infections recurring in 2 weeks, 2-3 wks of treatment and suppressive therapy throughout the pregnancy should be given.Post coital cephalexin 250mg or NFT 50 mg may be preventive.Perinephric or cortical abscesses may be seen.Although renal function is well maintained during acute pyelonephritis, some pregnant women develop acute renal failure.Renal biopsy may reveal focal microabscesses and recovery after antimicrobial therapy may be incomplete due to irreversible injury.

Acute Fatty Liver of Pregnancy (AFLP)
It is a rare complication of pregnancy.AFLP is unique to pregnancy.There is no predilection for any geographical area or race.It occurs more commonly in nullipara and primipara than multipara.It was first described in 1940 by Sheehan as an "acute yellow atrophy of the liver".The incidence of AFLP is 1 case per 20,000 births in the UK.Incidence of AFLP from non-UK studies based on hospital case series estimate 1 in 4000 deliveries to 1 in 16000 births.AFLP is associated with AKI in 60%.AFLP occurs in the third trimester or the immediate period after delivery however it can be seen as early as 26 weeks of gestation.(Buytaert,1996) It is due to mutation in enzyme for long chain 3 hydroxy acyl CoA dehydrogenase leading to disordered metabolism of fatty acids by mitochondria in the mother.The most common mutation found in acute fatty liver of pregnancy is the E474Q missense mutation (IJlst L,1997) This gene mutation is recessive; therefore, in non pregnant state, women have normal fatty acid oxidation.However, if the fetus is homozygous for this mutation, it will be unable to oxidize fatty acids.These acids are passed to the mother, who, because of diminished enzyme function, cannot metabolize the additional fatty acids.This results in hepatic strain leading to the development of AFLP, which can be relieved by delivery of the infant.The accumulation of long-chain 3-hydroxyacyl metabolites produced by the fetus or placenta is toxic to the liver and may be the cause of the liver disease.It is characterized by jaundice, hypoalbuminemia, mild renal failure, DIC, PIH, hypoglycemia, pancreatitis and encephalopathy.Laboratory findings may be consistent with disseminated intravascular coagulation (DIC), specifically, prolongation of prothrombin time, low fibrinogen, and low antithrombin levels.This results in a clinical picture similar to DIC; however, in AFLP, the values are abnormal, not due to consumption of the clotting factors but rather to decreased production by the damaged liver.Bilirubin levels are elevated.This elevation is primarily the conjugated form, with levels exceeding 5 mg/dL.This can result in jaundice, which is rarely seen in patients with other forms of pregnancy-related hepatic injury, including preeclampsia.The diagnosis should be suspected in a woman with preeclampsia who has hypoglycemia, hypofibrinogenemia, and a prolonged PTT in the absence of abruptio placentae, high bilirubin, normal/ high transaminases, microvesicular steatosis and rarely liver necrosis.Criteria for diagnosis of AFLP include six or more of the following features in the absence of another explanation: Vomiting, abdominal pain, polydipsia / polyuria, encephalopathy, elevated bilirubin, hypoglycemia, elevated urate, leucocytosis (>11x109/l), ascites or bright liver on ultrasound scan, elevated transaminases (aspartate aminotransferase or alanine aminotransferase >42 IU/l ), elevated ammonia, renal impairment, coagulopathy (prothrombin time >14 s or activated partial thromboplastin time >34 s), microvesicular steatosis on liver biopsy.(Ch'ng,2002).The liver biopsy is diagnostic but is not always feasible especially in patients with severe coagulopathy (Bacq, 2006) and it seldom influences acute management.Ultrasound and computed tomography have been used but false negative results are common.Renal biopsy shows Acute tubular necrosis (ATN).Delivery of the fetus, regardless of gestational age, is the only treatment for acute fatty liver of pregnancy (AFLP) once the diagnosis has been made.Treatment includes intravenous fluids, cryoprecipitate, Fresh frozen Plasma and glucose.It reverses with delivery.C section is preferred over vaginal delivery.Liver transplant is the treatment of choice in patients with liver necrosis.There is 20-25% maternal and fetal mortality and the syndrome can recur in subsequent pregnancies with a calculated genetic risk of 25%.The other causes of jaundice during pregnancy include cholestasis, cholelithiasis, viral hepatitis and pre-eclampsia with or without HELLP syndrome.Intrahepatic cholestasis of pregnancy may present during the third trimester but itching is the characteristic symptom and serum bilirubin concentration is rarely higher than 6mg/ dl.Cholelithiasis may occur at any time during pregnancy and is accompanied by pain in the right upper quadrant, and fever, and USG is usually diagnostic.Acute viral hepatitis in pregnancy presents as a systemic illness with fever, nausea, vomiting, fatigue, and jaundice, however, aminotransferase concentrations are markedly elevated (>500U/liter).The manifestations of pre-eclampsia are usually observed in the second half of pregnancy, whereas the symptoms of HELLP syndrome and AFLP frequently appear in the third trimester.The incidence of HELLP syndrome is much higher (1:5,000).Morbidity of the infant includes increased risk of cardiomyopathy, neuropathy, myopathy, nonketotic hypoglycemia, hepatic failure, and death associated with fatty acid oxidation defects in newborns.

Urinary tract obstruction
Functional hydronephrosis rarely cause renal failure.The diagnosis can be established in some cases by the normalization of renal function in the lateral recumbent position and its recurrence when supine.In some cases, either insertion of a ureteral catheter or delivery of the fetus is required.Rarely, acute urinary tract obstruction in pregnancy is induced by a kidney stone.

Antiphospholipid Syndrome (APS)
It refers to a syndrome characterized by arterial or venous thrombosis or specific pregnancy complications in women with laboratory evidence of antibodies to proteins bound to anionic phospholipids.International consensus conferences have proposed and revised classification criteria for definite APS ( Sapporo criteria) (Miyakis 2006).Definite APS is considered present if at least one of the following clinical criteria and at least one of the following laboratory criteria are satisfied.1. Clinical -(a)Thrombosis -Unequivocal imaging or histologic evidence of thrombosis in any tissue or organ, OR (b) Pregnancy morbidity -Otherwise unexplained death at ≥10 weeks gestation of a morphologically normal fetus, OR One or more premature births before 34 weeks of gestation because of eclampsia, preeclampsia, or placental insufficiency, OR Three or more embryonic (<10 week gestation) pregnancy losses unexplained by maternal or paternal chromosomal abnormalities or maternal anatomic or hormonal causes.2. Laboratory -The presence of antiphospholipid antibodies (aPL), on two or more occasions at least 12 weeks apart and no more than five years prior to clinical manifestations, as demonstrated by one or more of the following: (a)IgG and/or IgM aCL in moderate or high titer (>40 units GPL or MPL or > 99th percentile for the testing laboratory),(b)Antibodies to ß2-glycoprotein-I (ß2-GP-I) of IgG or IgM isotype at a titer >99th percentile for the testing laboratory when tested according to recommended procedures.(c)Lupusanticoagulant (LA) activity detected according to published guidelines Clinical manifestations include deep vein thrombosis, thrombocytopenia, livedo reticularis, stroke, superficial thrombophlebitis, pulmonary embolism, fetal loss, transient ischemic attack, and hemolytic anemia.In rare patients, APS results in multiorgan failure because of multiple blood vessel occlusions, a condition referred to as "catastrophic antiphospholipid syndrome" In patients with preeclampsia or the HELLP syndrome, catastrophic APS must be considered, in patients with histories of thrombosis or spontaneous abortions .Thrombotic renal disease occurs in a minority of patients with APS.Glomerular capillaries and other renal vessels, both arteries and veins of all sizes, can be affected.The disease may be silent or produce acute or chronic renal failure with proteinuria.APLS may cause glomerular lesions like membranous nephropathy, minimal change disease or proliferative glomerulonephritis.

Laboratory diagnosis
A history of a biologic false positive serologic test for syphilis (BFPTS) may be a clue to the presence of any type of aPL: aCL, ß2-GP-I antibodies, or an LA.However, because of the nonspecific nature of the BFPTS, the presence of one or more aPL should be confirmed with one of the tests (A)The presence of aPL may be demonstrated directly by: ELISA testing in the case of aCL and antibodies to ß2-GP-I and (B) Clotting assay that demonstrates effects of an aPL on the phospholipid-dependent factors in the coagulation cascade (LA test).The lupus anticoagulant phenomenon refers to the ability of aPL to cause prolongation of in vitro clotting assays such as the activated partial thromboplastin time (aPTT), the dilute Russell viper venom time (dRVVT), the kaolin clotting time or, the prothrombin time.This prolongation is not reversed when the patient's plasma is diluted 1:1 with normal plateletfree plasma.Treatment includes heparin, oral anticoagulants ie warfarin, antiplatelet drugs eg aspirin and clopidogrel and hydroxyl chloroquin.

Miscellaneous causes
The other miscellaneous causes of acute renal failure in pregnancy include Acute gastroenteritis, infections ,drugs and other causes similar to the varied causes of AKI in non pregnant states.

Pathology
Pathology of pregnancy related AKI varies with etiology.

Glomerular endotheliosis
It is the typical finding in PIH.Glomeruli look enlarged and the endothelial cells are swollen.This lesion was first described by Spargo.It resolves by the end of post partum period.

Acute tubular necrosis
May be seen in cases of ischemic or toxic insult.

Acute interstitial nephritis
May be commonly due to infection or drug.

Cortical necrosis
It is one of the dreaded histopathological lesions .50% of all cortical necrosis is pregnancy related.Causes include Abruptio (esp if concealed hemorrhage), septic abortion, placenta previa, prolonged IUD, or amniotic fluid embolism.It is commoner in post partum than antepartum AKI.Renal cortical necrosis may be patchy or total.The triad of anuria, gross hematuria, and flank pain seen in cortical necrosis is unusual in the other causes of renal failure in pregnancy.The diagnosis can be established by ultrasonography which shows a subcapsular hypoechoic band .Renal calcifications on plain film of the abdomen suggest cortical necrosis (6 weeks).CT scanning demonstrates hypoechoic or hypodense areas in renal cortex or may demonstrate cortical tram track calcification by 3 weeks.Angiogram shows abrupt cut off of vascularity which is called pruning.No specific therapy is effective.Many patients develop chronic kidney disease.In our study from Osmania General Hospital, Hyderabad, India over 7 years there were total of 105 patients with Renal cortical necrosis (RCN).The mean age was 28.125 ± 12.40 years.Forty one cases (39.04%) resulted from obstetric complications.The most common histology type of was patchy cortical necrosis in 65 patients (62%).All patients required dialysis and the mean duration of dialysis was 3 ± 1weeks.Thirty three (31.42%)patients progressed to end stage kidney disease while 3 underwent renal transplantation.10(9.5%)patients succumbed to the acute kidney injury (AKI).(ManishaSahay)

Vascular changes
May be seen in HUS with glomerular and arteriolar fibrinoid necrosis and thrombi.Glomeruli show mesangiolysis and glomerular simplification.

Dialysis in pregnancy
Early dialysis is necessary in pregnant women with renal failure and should be considered when the serum creatinine reaches 3.5 mg/dL or the glomerular filtration rate (GFR) is less than 20 mL/min or blood urea nitrogen is more than 100mg/dl.Longer, more frequent dialysis (20 h/wk) is associated with the best fetal outcome.Hemodialysis may therefore be necessary at least 5 days per week.Careful avoidance of hypotension is important.As pregnancy is a procoagulant state the dose of heparin needs to be increased.Peritoneal dialysis with smaller volumes and frequent exchanges is another option.Peritoneal dialysis may be difficult in the third trimester due to increased uterine size.Premature labor and fetal size that is small for the gestational age are typical in women who deliver on dialysis.Nutritional support that allows weight gains of 0.3 to 0.5 kg/wk should be maintained in the second and third trimesters.Although the spontaneous abortion rate is approximately 50% for pregnant women who require dialysis, the fetal survival rate for pregnancies that continue is as high as 71%.