Anterior Chamber Angle Assessment Techniques

The anterior chamber angle is the actual anatomical angle created by the root of the iris and the peripheral corneal vault. Within it lie the structures involved in the outflow passage of the aqueous, namely the trabecular meshwork and the Schlemm’s canal (figure 1). The depth of the angle in a healthy eye is approximately 30°, with the superior part usually less deep than the inferior half. However the depth is influenced by gender, age and refractive error. Female gender has the greatest influence on iridocorneal angle reduction, followed by age and spherical equivalent (Rufer et al.). The relationship of the iris plane to the cornea has a significant effect on the aqueous humor’s accessibility to its outflow drainage system. In eyes where the iris and corneal endothelium are “closed”against one another, the aqueous will not be drained causing an increase of the intraocular pressure (angle closure glaucoma) (Lens, 2008).


Introduction
The anterior chamber angle is the actual anatomical angle created by the root of the iris and the peripheral corneal vault.Within it lie the structures involved in the outflow passage of the aqueous, namely the trabecular meshwork and the Schlemm's canal (figure 1).The depth of the angle in a healthy eye is approximately 30°, with the superior part usually less deep than the inferior half.However the depth is influenced by gender, age and refractive error.Female gender has the greatest influence on iridocorneal angle reduction, followed by age and spherical equivalent (Rufer et al.).The relationship of the iris plane to the cornea has a significant effect on the aqueous humor's accessibility to its outflow drainage system.In eyes where the iris and corneal endothelium are "closed"against one another, the aqueous will not be drained causing an increase of the intraocular pressure (angle closure glaucoma) (Lens, 2008).Primary angle-closure glaucoma (PACG) is a leading cause of blindness worldwide (Quigley and Broman, 2006).Five overlapping conditions, not necessarily progressing in an orderly sequence, are usually described in this disease: angle closure suspect, intermittent (subacute) angle closure, acute angle closure, chronic angle closure and absolute angle closure (Kanski, 2007).At the earliest stage (angle closure suspect), eyes have narrow or occludable angles without raised intraocular pressure (IOP) or glaucomatous optic neuropathy.It has been estimated that 22% of the eyes with angle closure suspect progress to acute angle closure (Thomas et al., 2003a) whereas 28.5% progress to chronic angle closure over 5-10 years (Thomas et al., 2003b).Prophylactic laser iridotomy performed in eyes with narrow angles may halt the progression of the angle closure process and prevent development of PACG (Nolan et al., 2003).Similarly peripheral iridoplasty, which may also be used to break an attack of acute angle-closure unresponsive to medical therapy or laser iridotomy, can be successfully employed in nonacute situations in patients with angle-closure when laser iridotomy fails to deepen the anterior chamber angle, i.e. particularly in case of plateau iris (Ritch et al., 2007, Ng et al., 2008).Hence evaluation of anterior chamber angle (ACA) is of great importance to identify and treat those patients at risk for PACG.It is well known that the depth and volume of the anterior chamber diminish with age and are related to the degree of ametropia.Male subjects have larger anterior chamber dimensions than female subjects.Although grading of limbal chamber depth using van Herick's technique (see below) is commonly used as a surrogate for measuring ACA, gonioscopy has represented for many years the only method able to adequately quantify ACA.However this technique has a subjective and semi-quantitative nature, is hardly reportable due to the difficulty of obtaining good images and requires a good training to be performed properly.Moreover, in some circumstances such as plateau iris, the angle can be narrow despite a deep anterior chamber.For these reasons, with technology advancements, several new techniques have been proposed with the aim of providing a better imaging of the anterior segment.In this chapter we will show the clinical findings of each method reviewing the strengths and limitations of each approach.

van Herick test
Performed on the slit lamp without any additional aids, the van Herick test allows quick, not invasive assessment of anterior angle width.The technique was originally described by van Herich (Van Herick et al., 1969): a narrow slit of light is projected onto the peripheral nasal or temporal cornea at an angle of 60° as near as possible to the limbus.This results in a slit image on the surface of the cornea the width of which is compared with the peripheral anterior chamber depth ("black space") (figure 2).A four-point scale is then used, with each grade indicating the probability of angle closure.In grade 4 the anterior chamber depth (ACD) is ≥100% corneal thickness and the angle is wide open; in grade 3 it is > 25 to 50% and the angle is incapable of closure; in grade 2 it is 25% and the angle closure is possible; in grade 1 is < 25% and the angle closure is likely.Studies have shown suboptimal results when using van Herick test to screen for primary angle closure (Alsbirk, 1986), (Congdon et al., 1997), (Thomas et al., 1996): particularly it has been found that measurements performed at the nasal limbus tend to overestimate the angle width.To improve precision in quantification of ACD, Foster (Foster et al., 2000a) proposed a modified scheme in which the original grade 1 was sub-divided into 0%, 5%, and 15% corneal thickness, and a grade of 75% corneal thickness was added to compensate for the gap between the original grades 3 and 4.This seven-point grading system resulted in 99% sensitivity compared to gonioscopic evaluation; the interobserver agreement for this augmented grading scheme was good (weighted kappa 0.76).However it is hardly reproducible in clinical practice.

Smith method
Another optical technique used to determine ACD is Smith's slit-length method (Smith, 1979).It is performed using a standard slit-lamp.The illumination system is located in the subject's temporal field at an angle of 60° and the slit-beam is projected horizontally.If measuring the patient's right eye the right ocular of the slit lamp is used and vice versa for the left eye.A beam of approximately 1.5 mm thickness, with its orientation horizontal, is placed across the cornea.The procedure involves focusing the slit-beam on the corneal surface while an out of focus image of the slit-beam is observed on the iris/lens surface.The length of the slit-beam when the two corneal and iris/lens images are just touching is multiplied by a constant to give the ACD in millimetres (figure 3).In the original description of the method by Smith the constant was 1.40, while others (Barrett et al., 1996)  The Smith method has been validated by a number of authors (Barrett et al., 1996) (Douthwaite andSpence, 1986).It allows the clinician to obtain reliable estimates of axial ACD, without any attachments to the slit-lamp biomicroscope.The axial ACD estimates are accurate to within ± 0.25 mm (Smith, 1979), ± 0.2 mm (Jacobs, 1979) and± 0.33 mm (Barrett et al., 1996) as compared to pachymetry and to within ± 0.42 mm, as compared to ultrasonography.There is no effect of the central corneal thickness on the ACD estimate made using this method (Osuobeni et al., 2003), which has also a minimum inter and intra observer variation (Osuobeni et al., 2000).From birth to the age of 13 years, the mean value of ACD increases from 2.37 to 3.70 mm for Northern European boys, and from 2.39 to 3.62 mm for girls (Larsen, 1971).There is very little change in the mean anterior chamber depth from the teenage years to about 30 years, while there is a decline in the mean anterior chamber depth from 30 to 60 years, probably because of the increase of lens thickness (Fontana and Brubaker, 1980).Between this age range the typical value is around 2.5 mm.The depth and volume of the anterior chamber are also related to the degree of ametropia.Male subjects usually have larger anterior chamber dimensions than female subjects.There is a direct association between narrow anterior chamber angle and shallow anterior chamber depth (Wishart and Batterbury, 1992).
Consequently, the ACD quantification represents an indirect means of assessing the anterior chamber angle and identifying patients who are more likely to develop PACG.Usually eyes with ACD <2 mm are considered at risk (Wishart and Batterbury, 1992).Fig. 3. Measurement of the anterior chamber depth using Smith's method.Yellow arrow indicates the focused horizontal corneal-imaged slit and red arrow the out-of-focus iris/lens-imaged slit (see text for details)

Gonioscopy
Gonioscopy still represents the gold standard for assessment of the angle.This technique was first developed by Trantas in the late 1800s and subsequently modified by Koeppe and Barkan to allow a direct visualization of the structure of the anterior chamber angle with a contact lens (Friedman and He, 2008).Nowadays however indirect gonioscopy (which relies on mirrors or prisms to reflect light from the angle to the viewer) is usually preferred because of several advantages over direct gonioscopy: the patient can be examined at the slit lamp using a variable magnification and there is no astigmatic aberration.Two lenses are commonly employed: the Zeiss-type and the Goldmann-type.The Zeiss-type lens has a 9-mm diameter corneal surface (radius of curvature 7.72 mm) and doesn't require a coupling agent.The Goldmann-type has larger base diameter (corneal surface 12 mm and radius of curvature 7.38 mm) and requires a coupling agent (thick artificial tears or hydroxypropyl methylcellulose) when placed on the cornea.Although use of Zeiss-type lens needs more training and expertise, it has undoubted advantages because leaves the anterior segment clear for later viewing of the posterior pole and compresses the cornea centrally, which in turns allows for greater dynamic assessment of the angle structures.Moreover pressure on the larger base lenses can lead to compression over Schwalbe's line with a consequent alteration of angle's morphology.Since illumination conditions and degree of pupil dilation may dramatically alter angle configuration, a strict assessment protocol should be followed: the patient should look straight ahead and should be examined in a dark room, using a 1-mm beam with adequate illumination to visualize angle structures (Weinreb and Friedman, as cited in (Friedman and He, 2008).Three grading systems have been proposed for documenting angle findings seen in gonioscopy: Scheie (Scheie, 1957), Schaffer (Shaffer, 1960) and Spaeth (Spaeth, 1971) classification (table 1).In the Scheie scheme grade zero represents a wide open angle.Grade 1 is a "slightly narrow" angle and the iris root is not visible; grade 2 means that the ciliary body is not visible while grade 3 means that the posterior (pigmented) trabecular meshwork is not visible.Grade 4 is a closed angle and therefore no angle structures are visible.Scheie believed that persons with grade 3 and grade 4 angles were at greatest risk of PACG.The Shaffer system is currently the most popular grading system.It uses both angle width and angle structures to classify angle grade: this is confusing because sometimes width and structures seen may place an angle into different categories.In this grading system angles between 35 and 45 degrees are classified as grade 4, those between 20 and 35 as grade 3, those between 10 and 20 as grade 2 and those ≤10 as grade 1, with a closed angle (zero degrees) classified as grade 0. Angle width is often preferred to angle depth in the description of ACA, because the latter may differ in different locations.Taking into consideration the angle structures, Shaffer classification's grade 4 comprises all structures, grade 3 the structures up to the scleral spur, grade 2 up to the trabecular meshwork, in grade 1 only the Schwalbe's line is visible and in grade 0 none of the angle structures are visible.
Spaeth classification provides the most comprehensive approach to angle assessment.This classification includes three components: angular width of angle recess, configuration of the peripheral iris, insertion site of the iris root.The width of the angle recess is graded from 10 to 40 degrees.The iris configuration is reported as ''r'' (regular), ''s'' (steep, as in plateau iris configuration), or ''q'' (queer, or backward, bowing as may occur in pigment dispersion syndrome).The insertion of iris root ranges from A -anterior to the Schwalbe's line, Bbehind Schwalbe's line, but anterior to scleral spur, C -posterior to scleral spur (i.e., scleral spur visible, but not ciliary body), D -ciliary body visible, and E -large amount of ciliary body visible.When the iris is appositional with the angle, the "apparent" iris insertion, seen without indentation, is noted as a letter placed in parenthesis, while the "actual" insertion is noted with a letter not placed in parenthesis.Finally the Spaeth system rates also the pigment of the posterior trabecular meshwork at 12 o'clock from 0 to 4+ (black pigmented meshwork) and the presence or absence of peripheral anterior synechiae (PAS).An example of wide open angle is given in figure 4.  (Spaeth et al., 1995).
A quantitative grading of the angle has been proposed by some authors (Cockburn, 1980, Congdon et al., 1999), but has not gained popularity in clinical practice.Once an angle is viewed and it is determined that there is iridotrabecular contact, it is necessary to determine whether the angle is appositionally closed or if there are permanent PAS.To pursue this task a gentle indentation with the goniolens is performed ("dynamic gonioscopy").By indenting the central cornea (usually with a Zeiss-type lens) the aqueous is displaced into the peripheral anterior chamber where it bows the iris posteriorly and widens the chamber angle.This widening differentiates areas where the peripheral iris is permanently adherent to the peripheral cornea (i.e.PAS) from areas where the iris is merely reversibly apposed to the peripheral cornea.
Caution must be taken in distinguishing PAS from iris processes.Iris processes, either plastered across the surface of the angle or bridging from the peripheral iris to the angle structures, are pigmented strands continuous with and histologically identical to the iris.These are a normal variant and have no effect upon aqueous outflow.On the contrary PAS are abnormal adhesions of the peripheral iris to the angle structure that, if extensive enough, can eventually reduce trabecular outflow.Usually PAS tend to be wider (at least half of 1 clock hour in width) and are present to the level of the trabecular meshwork or higher.
Examples of PAS include the fibrovascular membrane formed in neovascular glaucoma, proliferating abnormal endothelial cells in the iridocorneal endothelial (ICE) syndromes, epithelialization of the angle due to epithelial ingrowth, or inflammatory trabecular and keratic precipitates in contact with an inflamed iris in uveitis.
The gonioscopic criteria for an occludable angle usually include: 1) trabecular meshwork invisible in 270° or more of the entire angle in the primary position of gaze without indentation and / or 2) angular width less than 20 degrees by the Shaffer grading (Kim and Jung, 1997).Often these criteria are used to identify angles that require treatment (i.e.iridotomy), although there is still no unanimous consensus (Friedman, 2001, Foster et al., 2002).

Pentacam
The Pentacam (Oculus, Inc., Lynnwood, WA, USA) is a rotating Scheimpflug camera with a short-wavelength slit light (475 nm, blue light-emitting diode laser) that is able to take 25 slit images of the anterior segment of the eye in 2 seconds with 500 true elevation points in each image.Any eye movement is detected (and the results corrected) by a second camera.A three-dimensional model of the anterior segment can therefore be built with the obtained data including the corneal thickness, corneal topographic parameters, central ACD, anterior chamber angle (ACA), anterior chamber volume (ACV) and other parameters.Interestingly the software doesn't require any manual initiation.Two chamber angles for each chosen meridian are provided.It is noteworthy to mention that ACA is calculated by lengthening the posterior cornea and the iris contour to compute the chamber angle using an interpolation method, because Pentacam cannot image the angle recess and scleral spur.
Several studies have investigated the reliability of Pentacam (and other Scheimpflug cameras) in measuring ACA.Lam (Lam et al., 2002) found on 25 healthy subjects with open angles that the 95% limit of agreement on repeat measurements of angle width was 5°, and inter-observer agreement was 6º.Rabsilber showed good reliability of Pentacam in assessing ACA in 76 healthy volunteers (Rabsilber et al., 2006).Although measurement of the ACA obtained with the Pentacam seem to correlate significantly with Shaffer's grade determined by gonioscopy, a certain discrepancy has been reported between ACA and ACD measured by Pentacam and ultrasound biomicroscopy (UBM) or anterior-segment optical coherence tomography (AS-OCT) in eyes with a narrow angle (Liang et al.) (Kurita et al., 2009) (Mou et al.).This may be due to the inability of the Pentacam to visualize the most peripheral part of the iris.Alternatively the placement of an eye cup, which is necessary for UBM examination, may be responsible of a flattening of the cornea which in turns leads to an artificial reduction of the ACD.Hence ACV seems to be the most efficient parameter to screen patients with POAG or POAG suspect using the Pentacam (Kurita et al., 2009).However although Pentacam non-contact approach to angle assessment is highly appealing for screening purposes, it is limited to visualization of only the angle recess.Scheimpflug photography indeed does not display the retroiridal structures or the ciliary body, which are of great interest in glaucoma diagnosis.

Ultrasound Biomicroscopy (UBM)
Ultrasound-based diagnostic imaging uses a probe containing a piezoelectric transducer to emit a sound wave which propagates through tissues and is partially reflected -echoes-from anatomic structures differing in acoustic impedance (density × speed of sound).Some of the echoes return to the transducer and are converted back into voltages and amplified.The range of each echo is proportional to the time delay between sound wave emission and echo return, specifically, r = ct/2, where r is the range, c is the speed of sound (1532 m/s at 37°C in normal saline) and t is the time (Ursea and Silverman).
Each pulse/echo event thus provides information along one line of sight.By mechanically scanning the probe, information along an ordered series of lines is obtained.By converting echo amplitude into pixel intensity, a 2D cross-sectional B-scan image is then produced.Ultrasonic imaging resolution improves by increasing the frequency of the transducer.However higher frequencies produce a correspondingly smaller wavelength which is less able to penetrate the tissues.
Ultrasound systems utilizing probes of approximately 35 MHz or more have come to be known as "ultrasound biomicroscopy" (UBM) or 'very high-frequency ultrasound systems'.Such systems have a tissue penetration of only 5 mm but provide lateral and axial resolutions approximately of 40 and 20 microns, respectively.They allow therefore for a more detailed assessment of the anterior ocular structures than was available using traditional B-scan ultrasound.UBM systems are now produced by numerous companies, with probes ranging from 50 to 80 MHz.Handheld UBM probes are now often equipped with acoustically transparent, fluid-filled 'bubble tips' that can be placed directly onto the globe.These obviate the use of water-baths or scleral shells for acoustic coupling, greatly simplifying the examination and allowing the patient to be examined in a sitting position.UBM provides both quantitative and qualitative information on the anterior segment of the eye.Pavlin and coworkers carried out the first clinical UBM study of the ocular structures in glaucomatous patients in the early 1990s (Pavlin et al., 1992).Subsequently many authors proposed different biometric parameters to characterize the angle and anterior segment.The most common include: angle-opening distance (AOD), trabecular-iris angle (TIA), trabecular-ciliary process distance (TCPD), iris thickness (ID), angle-recess area (ARA), iris ciliary process distance (ICPD), iris-lens contact distance (ILCD) (see Table 2 and figure 5 for details).AOD and ARA can be measured at various distances from the sclera spur.Theoretically, 500 μm is the appropriate distance because it approximates the length of the trabecular meshwork.However, a longer measurement distance of 750 μm uses information from a large region of the image and may be more robust, especially for ARA, since it is less affected by local iris surface undulations.Henzan and coworkers studied the performance of the UBM parameters in differentiating primary angle closure/primary angle closure suspect from non-occludable angle eyes through the receiver operating characteristic (ROC) curve and the area under the curve (AUC).They found that AOD 500 and TIA under light conditions had the greatest AUC of 0.94.The ideal cutoff values for the AOD 500 and TIA under light conditions determined with the Youden index (=sensitivity -[1 -specificity]) were 0.17 mm (sensitivity, 0.82; specificity, 0.96) and 15.2 degrees (sensitivity, 0.83; specificity, 0.93), respectively (Henzan et al.).A limitation of these findings is that UBM measurements of angle structures can be influenced by a number of variables including patient's age and gender (Friedman et al., 2008), direction of gaze, accommodation, room illumination, variation in image acquisition (position of the probe, meridians scanned) (Friedman and He, 2008).One would expect high variability in the measurements because of the partly subjective nature of the caliper placement on visualized anatomic landmarks.On the contrary the reported reproducibility of analyses on single UBM images seems to be pretty good.
Marchini showed high reproducibility in a paper comparing UBM parameters in angle closure patients (range of coefficient of variation 1.4 -16%) (Marchini et al., 1998).
Even better reproducibility was reported by Gohdo when measuring the ciliary body thickness (CBT) one and two millimeters posterior to the scleral spur (coefficient of variation < 2.5%) (Gohdo et al., 2000).
In any case image analysis using calipers to mark each structure takes a large amount of time due to the need to place a cursor at each point for any given measurement.
To overcome this issue, Ishikawa and colleagues created a semi-automated program (UBM Pro 2000, Paradigm Medical Industries, Salt Lake City, UT, USA) that provides several important parameters once the scleral spur is identified (figure 6) (Ishikawa et al., 2000).(Friedman et al., 2003) for NOA (Yoo et al., 2007) for OA ICPD Distance from the posterior iris surface to the ciliary process perpendicular 500 μm from scleral spur 0.39± 0.21 0.40± 0.10 (Sihota et al., 2005) ILCD Length of contact between surfaces of lens and iris 0.79±0.220.98± 0.41 (Sihota et al., 2005) *All values (mean±standard deviation) are in mm except TIA which is in degrees and ARA500 which is in mm 2 .Abbreviations: AOD=angle-opening distance; TIA=trabecular-iris angle; TCPD=trabecular-ciliary process distance; ID:iris thickness; ARA=angle-recess area; ICPD=iris ciliary process distance; ILCD=iris-lens contact distance; Table 2. Biometric parameters used in UBM for characterizing the angle and anterior segment in subjects with an occludable/nonoccludable angle The software calculates AOD 250 , AOD 500 , ARA 750 and performs linear regression analysis of consecutive AODs, producing two figures: the acceleration and the y-intercept.Acceleration tells how rapidly the angle is getting deeper, using the tangent of the angle instead of degrees as the unit.The y-intercept refers to the distance between the scleral spur and the iris surface along the perpendicular to the trabecular meshwork plane.Acceleration and the y-intercept can be negative numbers.A negative number for acceleration means that the angle has an almost normal configuration at its peripheral part and becomes very shallow or is attached to the cornea at its central part.A negative yintercept means that the angle recess is very shallow or is attached to the cornea at its periphery, whereas it is relatively wide centrally.According to Ishikawa and colleagues this software dramatically improves the overall reproducibility, with a coefficient of variation ranging between 7.3 and 2.5 for the various parameters (Ishikawa et al., 2000).
UBM gives also valuable qualitative information which helps in the diagnosis and in the management of several ocular diseases.In plateau iris syndrome UBM well demonstrates the ciliary body anteriorly positioned compressing the iridocorneal angle and placing the peripheral iris in apposition to the trabecular meshwork (figure 7).

Anterior segment optical coherence tomography (AS-OCT)
Optical coherence tomography (OCT) of the eye was first described by Huang and coworkers at the Massachusetts Institute of Technology (Boston, MA, USA) in 1991 (Huang et al., 1991).OCT uses a near-infrared light that is directed throughout ocular tissues.While most of the light is absorbed by the tissues or scattered, a small portion is reflected and collected by an interferometer in order to produce an image.In time-domain OCT, the reference mirror is mechanically scanned in the range axis, and this allows determination of the range to optical reflections along the tissue path, which are represented by interference fringes in the OCT signal.
OCT was initially developed only for retinal imaging; in 1994 Izatt et al. (Izatt et al., 1994) for the first time used it also for imaging the anterior chamber (anterior segment OCT, AS-OCT).Since then, AS-OCT has rapidly become popular for ACA assessment.
Originally anterior and posterior segment imaging used the same wavelength (830 nm).Subsequently a longer wavelength of 1310 nm was preferred for AS-OCT.This increases the depth of penetration by reducing the amount of light scattered by the sclera and limbus, allowing for visualization of the ACA morphology in greater detail.In addition, the 1310 nm light incident on the cornea is strongly absorbed by water in the ocular media, with only 10% reaching the retina.This enables the AS-OCT to utilize higher power, enhancing imaging speed and eliminating motion artifacts (Quek et al.).The Visante™ OCT (Carl Zeiss Meditec, Dublin, CA, USA) and the SL-OCT (Heidelberg Engineering, GmbH, Dossenheim, Germany) are 2 commercially available devices which use this wavelength providing an axial and transverse resolution of 18 μm and 60 μm, respectively, for the Visante and <25 μm and 20-100 μm for the SL-OCT (Quek et al.).SL-OCT incorporates OCT technology into a modified slit-lamp biomicroscopy system: this requires slower image acquisition speed and more operator skills.
More recently the new frequency (Fourier) domain OCTs have been developed, where the broadband signal is broken into a spectrum using a grating or linear detector array (i.e.sensitive detectors arranged in grating or single row), and depth is determined from the Fourier transform of the spectrum without motion along the reference arm (Ursea and Silverman).The fast readout speed of the detectors (typically tens of kilohertz) allows acquisition at video frame rates (30 fps) while the multiplexed scheme provides a signal-tonoise ratio (SNR) advantage over time domain OCT (TD-OCT).Fourier (also called Spectral) domain OCTs (SD-OCTs) allows scans at a rate of 26,000 A-scans per second and more images to be taken in a single pass.These devices produce therefore detailed cross-sectional images of structures at an axial resolution of 5 μm and a transverse resolution of 15 μm.The RTVue (Optovue Inc.,Fremont, CA, USA), the Cirrus high-definition OCT (HD-OCT) 4.0 (Cirrus; Carl Zeiss Meditec Inc.) and the OPKO Spectral OCT SLO (OPKO Health, Inc.) are all SD-OCT systems that can be used for either retinal or anterior segment imaging (when used with a corneal adaptor module).AS-OCTs provide same type of ACA measurements of UBM, with the same advantages and limitations (i.e. they are influenced by patient's age and gender, direction of gaze, accommodation, room illumination, meridians scanned).Furthermore, likewise UBM, some AS-OCTS have a built-in semi-automated software which offers the most common biometric parameters of ACA after the manual localization of the scleral spur (figure 9) (table 3).Contradictory results are present in literature on the agreement between UBM and AS-OCT in quantitative ACA measurement and detection of narrow angles.Some authors have found the two methods to be quite similar (Radhakrishnan et al., 2005, Dada et al., 2007); others have shown poor agreement (Mansouri et al.).In a study including 32 patients Wang et al. have reported that low-resolution OCT is similar to UBM for most of the studied angle measurements, while high-resolution OCT tends to give higher measurements than both low-resolution OCT and UBM.Furthermore AS-OCT measurements seem more reproducible than those from UBM (Wang et al., 2009).Likewise UBM, AS-OCT may be used for qualitative evaluation of ACA in a variety of ocular diseases (plateau iris syndrome, pigment dispersion syndrome, etc.) and it is undoubtedly safer than UBM in the evaluation of filtering blebs because AS-OCT is non-contact technique.(Nolan et al., 2007, See et al., 2007) (Pekmezci et al., 2009).AS-OCT tends indeed to detect more closed ACAs than gonioscopy, particularly in the superior and inferior quadrants (Nolan et al., 2007, Sakata et al., 2008a).
Angle recess area at 750 μm from scleral spur (ARA 750 ) and angle-opening distance at 500 μm from the scleral spur (AOD 500 ) seem to have the highest correlation with gonioscopy (Pekmezci et al., 2009).
Radhakrishnan indicated an AOD 500 cutoff of 190 μm for detecting occludable angles (Radhakrishnan et al., 2005).As for UBM, also for AS-OCT angle classification hinges on accurate localization of the scleral spur, as it is used as the reference point for all the other quantitative measurements.However, this localization is not always easy to be found possibly generating non-negligible intra-and inter-observer variance.
The sclera spur can be defined as the point where there is a change in curvature of the inner surface of the angle wall, often appearing as an inward protrusion of the sclera.
Studies investigating the visibility of the sclera spur with AS-OCT showed a visualization between 70% and 78.9% of analyzed images (Sakata et al., 2008b) (Wong et al., 2009).Most of the cases in which the scleral spur could not be detected occurred in images in which the internal surface of the sclera formed a smooth continuous line (with no inward protrusion of the sclera or change in its curvature) or in images with suboptimal quality.Less frequently, the scleral spur was difficult to identify owing to an atypical contour of the inner corneoscleral wall.Despite the possible difficulty in localization of sclera spur, several studies have shown low intra-and inter-observer variability of AS-OCT ACA measurements, which tends to increase only when AS-OCT image acquisitions are performed by less-experienced operators (Khor et al., Tan et al., Muller et al., 2006, Li et al., 2007) In a recent study the range of intraobserver variability in image analysis was from 9.4% to 12.5% in the experts and from 4.2% to 17.4% in the non-experts.Inter-observer variability was 10.7% in the experts and 10.2% in the non-experts.The reproducibility was high, 0.875 and 0.942 in the experts and 0.906 in the non-experts (Tan et al.).

Conclusions
Several new technologies are becoming more and more popular for the assessment of the angle and anterior segment.They can provide useful additional qualitative information to those obtained with the traditional tools (slit-lamp and gonioscopy).Furthermore they can offer also precise ACA measurements which however are often not comparable each other.
Hence we believe that one should build its normative data -using gonioscopy as reference standard-to use them for screening for angle closure purposes.However, as technologies evolve, it is likely that the diagnostic performance of different techniques/instruments may soon reach acceptable specificity and sensitivity levels for mass screening for angle closure.(1980).A new method for gonioscopic grading of the anterior chamber angle.Am J Optom Physiol Opt, Vol. 57, No. 4, (Apr 1980) pp. (258-261), 0093-7002 (Print) Congdon, N.G., Spaeth, G.L., Augsburger, J., Klancnik, J., Jr., Patel, K. & Hunter, D.G. (1999).

Fig. 1 .
Fig. 1.Diagram of the anatomical structures forming the iridocorneal angle

Fig. 2 .
Fig. 2.An example of van Herick technique for assessing anterior chamber depth (a grade 4 is shown, see text) have p r o p o s e d m o r e r e c e n t l y 1 .3 1 .B o t h c o n s t a n t s h a v e b e e n d e t e r m i n e d u s i n g a n o p t i c a l pachymetry.

Fig. 4 .
Fig. 4. Gonioscopy of a wide open angle.All angle structures are visible.From down to top:ciliary body band with some iris processes reaching the trabecular meshwork, scleral spur, pigmented (posterior) trabecular meshwork, nonpigmented (anterior) trabecular meshwork and Schwalbe's line delineated by some scattered pigment Not many studies have been published on the reliability of the angle grading systems.Some authors have reported a weighted k values for inter-observer reproducibility of Shaffer classification in the ranges of 0.6 using a Goldmann-style lens(Foster et al., 2000b) (Aung et  al., 2005).The Spaeth system has shown high reproducibility and comparability to UBM in 22 patients(Spaeth et al., 1995).A quantitative grading of the angle has been proposed by some authors(Cockburn, 1980,  Congdon et al., 1999), but has not gained popularity in clinical practice.Once an angle is viewed and it is determined that there is iridotrabecular contact, it is necessary to determine whether the angle is appositionally closed or if there are permanent PAS.To pursue this task a gentle indentation with the goniolens is performed ("dynamic gonioscopy").By indenting the central cornea (usually with a Zeiss-type lens) the aqueous is displaced into the peripheral anterior chamber where it bows the iris posteriorly and widens the chamber angle.This widening differentiates areas where the peripheral iris is permanently adherent to the peripheral cornea (i.e.PAS) from areas where the iris is merely reversibly apposed to the peripheral cornea.Caution must be taken in distinguishing PAS from iris processes.Iris processes, either plastered across the surface of the angle or bridging from the peripheral iris to the angle structures, are pigmented strands continuous with and histologically identical to the iris.These are a normal variant and have no effect upon aqueous outflow.On the contrary PAS are abnormal adhesions of the peripheral iris to the angle structure that, if extensive enough, can eventually reduce trabecular outflow.Usually PAS tend to be wider (at least half of 1 clock hour in width) and are present to the level of the trabecular meshwork or higher.

Fig. 5 .
Fig. 5. Diagram illustrating several biometric descriptors of the angle, including angleopening distance (blue line), iris thickness (yellow line), trabecular-iris angle (red line), trabecular-ciliary process distance (green line) and angle recess area (light blue area).In this example measurements are made 500 μm from the sclera spur

Fig. 7 .
Fig. 7. Ultrasound biomicroscopy view of an eye with plateau iris syndrome In pigment dispersion syndrome UBM shows an open angle, a characteristic concave iris and a ciliary body rotated posteriorly (figure 8).

Fig. 8 .
Fig. 8. Ultrasound biomicroscopy view of an eye with pigment dispersion syndrome Angle recession, intraocular foreign bodies, ciliary body cysts are also easily detectable by UBM.Lastly UBM may represent a useful tool for the planning and guidance of glaucoma surgery, including the evaluation of filtering blebs, sclerectomy and canaloplasty, as well as the diagnosis and evaluation of postoperative complications.

Table 1 .
Angle grading systems