Impact on Mechanical Properties of 10 versus  20 Minute Treatment of Human Pericardium  with Glutaraldehyde in OZAKI Procedure

Luca Koechlin; Giuseppe Isu; Vladislav Borisov; Diana Robles Diaz; Friedrich S. Eckstein; Anna Marsano; Oliver Reuthebuch

doi:10.5761/atcs.nm.20-00125

New Methods

Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure

Luca Koechlin, Giuseppe Isu, Vladislav Borisov, Diana Robles Diaz, Friedrich S. Eckstein, Anna Marsano, Oliver Reuthebuch

Author information

Luca Koechlin
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Giuseppe Isu
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Department of Biomedicine, University of Basel, Basel, Switzerland
Vladislav Borisov
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Department of Biomedicine, University of Basel, Basel, Switzerland
Diana Robles Diaz
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Department of Biomedicine, University of Basel, Basel, Switzerland
Friedrich S. Eckstein
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Department of Biomedicine, University of Basel, Basel, Switzerland
Anna Marsano
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland
Department of Biomedicine, University of Basel, Basel, Switzerland
Oliver Reuthebuch
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland

Keywords: pericardium, OZAKI, mechanical stability

JOURNAL OPEN ACCESS

2021 Volume 27 Issue 4 Pages 273-277

DOI https://doi.org/10.5761/atcs.nm.20-00125

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Abstract

Purpose: The aim of this study was to analyze the effects of 10-minute (standard term) versus 20-minute treatment with glutaraldehyde (GA) on mechanical stability and physical strength of human pericardium in the setting of the OZAKI procedure.

Methods: Leftover pericardium (6 patients) was bisected directly after the operation, and one-half was further fixed for 10 additional minutes. Uniaxial tensile tests were performed and ultimate tensile strength (UTS), ultimate tensile strain (uts), and collagen elastic modulus were evaluated.

Results: Both treatments resulted in similar values of uniaxial stretching-generated elongations at rupture (10 minutes 25 ± 7 % vs. 20 minutes: 22 ± 5 %; p = 0.05), UTS (5.16 ± 2 MPa vs. 6.54 ± 3 MPa; p = 0.59), and collagen fiber stiffness (elastic modulus: 31.80 ± 15.05 MPa vs. 37.35 ± 15.78 MPa; p = 0.25).

Conclusion: Prolongation of the fixation time of autologous pericardium has no significant effect on its mechanical stability; thus, extending the intraoperative treatment cannot be recommended.

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