To evaluate the phylogenetic relationships among Roegneria amurensis, R. ciliaris and R. japonensis, hybridizations among them were carried out. In the hybrids of R. amurensis × R. ciliaris, R. japonensis × R. amurensis and R. ciliaris × R. japonensis, average chromosome associations were 0.38 I 13.83 II, 0.18 I 13.90 II and 0.15 I 13.91 II, respectively. All the hybrids were partially fertile with stainable pollen grains and the fertility was 82%, 88% and 95%, respectively. The results indicated that the three species share the same basic genomes, StStYY and they are closely related. Combined with morphology and geographic distribution, R. amurensis and R. japonensis are treated as a subspecies and a variety of R. ciliaris, respectively.