Effects of Melatonin on Gene Expression of IVM/IVF Porcine Embryos

Jang, H. Y.; Kong, H. S.; Choi, K. D.; Jeon, G. J.; Yang, B. K.; Lee, C. K.; Lee, H. K.

doi:2005.18.1.17

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2005;18(1): 17-21.
https://doi.org/10.5713/ajas.2005.17 Published online April 19, 2005.

Effects of Melatonin on Gene Expression of IVM/IVF Porcine Embryos

H. Y. Jang, H. S. Kong, K. D. Choi, G. J. Jeon, B. K. Yang, C. K. Lee, H. K. Lee

Abstract

The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR. Porcine embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% CO2 and 20% O2 at 37째C in NCSU23 medium. Melatonin was added to medium at concentration of 1nM, 5 nM, and 10 nM. When treated with 1nM (39.0%) of melatonin, the developmental rate of embryos beyond the morula stage were higher than that of control group (31.0%) (p<0.05). Number of inner cell mass and tropectoderm cell in control (23.0 0.5 and 17.3 0.8), 1 nM (23.6 0.6 and 19.0 0.5), and 5 nM (23.3 1.1 and 16.3 0.8) treated with melatonin were higher than in 10 nM (20.0 0.5 and 13.3 0.8) treated with melatonin (p<0.05). To develop an mRNA phenotypic map for the expression of catalase, bax and caspase-3, single cell RT-PCR analysis were carried out in porcine IVM/IVF embryo. Catalase was detected in 0, 1 and 5 nM supplemented with melatonin, but bax and caspase-3 were detected in 10 nM treated with melatonin.

Keywords: Porcine Embryo; RT-PCR; Catalase; Bax; Caspase-3; Melatonin