RESEARCH PAPER
Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater
1
University of Veterinary Medicine and Pharmacy, Košice, Slovakia
2
Faculty of Medicine, Department of Medical Microbiology and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia
3
Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovakia
Corresponding author
Ann Agric Environ Med. 2015;22(3):447-450
KEYWORDS
ABSTRACT
Introduction and objective:
Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coli and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated from an urban wastewater treatment plant.
Material and Methods:
Wastewater samples and artificially added polystyrene pellets were used as the source for E. coli isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, qnrS, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper.
Results:
ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and qnrS genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant E. coli was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production.
Conclusions:
Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, qnrS genes and virulence factors present a potential environmental health risk.
Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coli and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated from an urban wastewater treatment plant.
Material and Methods:
Wastewater samples and artificially added polystyrene pellets were used as the source for E. coli isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, qnrS, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper.
Results:
ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and qnrS genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant E. coli was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production.
Conclusions:
Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, qnrS genes and virulence factors present a potential environmental health risk.
REFERENCES (25)
1.
Marti E, Variatza E, Balcazar JL. The role of aquatic ecosystems as reservoirs of antibiotic resistance. Trends Microbiol. 2014; 22(1): 36–41.
2.
Majtan J, Majtanova L, Xu M, Majtan V. In vitro effect of subinhibitory concentrations of antibiotics on biofilm formation by clinical strains of Salmonella enterica serovar Typhimurium isolated in Slovakia. J Appl Microbiol. 2008; 104(5): 1294–1301.
3.
Naves P, del Prado, G, Huelves L, Gracia M, Ruiz V, Blanco J, Dahbi G, Blanco M, del Carmen Ponte M, Soriano F. Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains. Microb Pathog. 2008; 45(2): 86–91.
4.
Kmet V, Drugdova Z, Kmetova M, Stanko M. Virulence and antibiotic resistance of Escherichia coli isolated from rooks. Ann Agric Environ Med. 2013; 20(2): 273–275.
5.
VET01S2: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals. CLSI 2013.p.1–168.
6.
Gregova G, Kmetova M, Kmet V, Venglovsky J, Feher A. Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse. Ann Agric Environ Med. 2012; 19(1): 75–77.
7.
Stepanović S, Vuković D, Dakić I, Savić B, ŠvabićVlahovic M. A modified microtiterplate test for quantification of staphylococcal biofilm formation. J Microbiol Methods. 2000; 40(2): 175–179.
8.
Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods. 2008; 72(2): 157–165.
9.
Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTXM extendedspectrum ßlactamases. J Antimicrob Chemother. 2005; 57(1): 154–155.
10.
PérezPérez PJ, Hanson ND. Detection of PlasmidMediated AmpC betaLactamase Genes in Clinical Isolates by Using Multiplex PCR. J Clin Microbiol. 2002; 40(6): 2153– 2162.
11.
Robicsek A, Strahilevitz J, Sahm DF, Jacoby GA, Hooper DC. Qnr prevalence in ceftazidime resistant Enterobacteriaceae isolates from the United States. Antimicrob Agents Chemother. 2006; 50(8): 2872–2874.
12.
Mazel D, Dychinco B, Webb VA, Davies J. Antibiotic resistance in the ECOR collection: Integrons and identification of a novel aad gene. Antimicrob Agents Chemother. 2000; 44(6): 1568–1574.
13.
Doyle D, Peirano G, Lascols Ch, Lloyd D, Churcha DL, Pitout JDD. Laboratory detection of Enterobacteriaceae that produce carbapenemases. J Clin Microbiol. 2012; 50(12): 3877–3880.
14.
Johnson JR, Stell AL. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis. 2000; 181(1): 261–272.
15.
Foley SL, Home SM, Giddings CW, Robinson M, Nolan LK. Iss from a virulent avian Escherichia coli. Avian Dis. 2000; 44(1): 185–191.
16.
Dozois CM, DhoMoulin M, Brée A, Fairbrother JM, Desautels C, Curtiss III. R. Relation between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the tsh genetic region. Infect Immun. 2000; 68(7): 4145–4154.
17.
Le Bouguénec C, Archambaud M, Labigne A. Rapid and specific detection of the pap, afa, and sfa adhesinencoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J Clin Microbiol. 1992; 30(5): 1189–1193.
18.
Gordon DM, O’Brien CL. Bacteriocin diversity and the frequency of multiple bacteriocin production in Escherichia coli. Microbiology 2006; 152: 3239–3244.
19.
Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000; 66(10):4555–4558.
20.
Zurfluh K , Hächler H, NüeschInderbinen M, Stephan R. Characteristics of extendedspectrum βlactamase and carbapenemaseproducing Enterobacteriaceae Isolates from rivers and lakes in Switzerland. Appl Environ Microbiol. 2013; 79(9): 3021–3026.
21.
BurkowskaBut A, Swiontek Brzezinska M, Walczak M. Microbiological contamination of water in fountains located in the city of Toruń, Poland. Ann Agric Environ Med. 2013; 20(4): 645–648.
22.
Woodford N, Wareham DW, Guerra B, Teale Ch. Carbapenemaseproducing Enterobacteriaceae and nonEnterobacteriaceae from animals and the environment: an emerging public health risk of our own making? J Antimicrob Chemother. 2014, 69(2): 287–291.
23.
Poirel L, Stephan R, Perreten V, Nordmann P. The carbapenemase threat in the animal world: the wrong culprit. J Antimicrob Chemother. 2014; 69(7): 2007–2008.
24.
Mliji el M, Hamadi F, Latrache H, Cohen N, El Ghmari A, Timinouni M. Association between plasmid carrying an expandedspectrum cephalosporin resistance and biofilm formation in Escherichia coli. New Microbiol. 2007; 30(1): 19–27.
25.
Pratt LA, Kolter R. Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol. 1998; 30(2): 285–293.
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