Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

Maria Surma, Tadeusz Adamski, Wojciech Święcicki, Paweł Barzyk, Zygmunt Kaczmarek, Anetta Kuczyńska, Karolina Krystkowiak, Krzysztof Mikołajczak, Piotr Ogrodowicz

Abstract


The aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed and yellow lupin cultivars. Embryos dissected from mature but still-green seeds were cultured in vitro on two modified MS media and under three temperature regimes. Shoot and root lengths of regenerated plants were measured after 7, 14 and 21 days of culture. For pea plants full-strength MS medium with 4 g l−1 agar and temperature 22/ 20°C (day/night) appeared to be the most conducive to shoot and root development, whereas for lupin plants lower temperatures were more propitious: 12°C in the dark for narrow-leafed lupin and 16/ 12°C (day/night) for yellow lupin. Almost all the cultured embryos developed into plants, but not all the regenerated plants survived acclimation to ex vitro conditions.

Keywords


Lupinus angustifolius; Lupinus luteus; Pisum sativum; embryo culture

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References


Pickering RA, Devaux P. Haploid production: approaches and use in plant breeding. In: Shewry PR, editor. Barley: genetics, biochemistry, molecular biology and biotechnology. Wallingford: C.A.B. International; 1992. p. 519–547.

Thomas WTB, Forster BP, Gertsson B. Doubled haploids in breeding. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I, editors. Doubled haploid production in crop plants: a manual. Dortrecht: Kluwer Academic Publishers; 2003. p. 337–349.

Wędzony M, Forster BP, Żur I, Golemiec E, Szechyńska-Hebda M, Dubas E, et al. Progress in doubled haploid technology in higher plants. In: Touraev A, Forster BP, Jain SM, editors. Advances in haploid production in higher plants. Berlin: Springer; 2008. p. 1–34.

Rybczyński JJ, Podyma E. Micropropagation of some Lupinus species from seedling explants. Genet Pol. 1993;34(3):237–247.

Rybczyński JJ, Podyma E. Preliminary studies of regeneration via somatic embryogenesis induced on immature cotyledons of white lupin (Lupinus albus L.). Genet Pol. 1993;34:249–257.

Rybczyński JJ. An assessment of the present studies of in vitro culture of lupins. In: Towards the 21st century. Proceedings of the 8th international lupin conference, Asilomar, California, USA, May 11–16 1996.

Canterbury, New Zealand: International Lupin Association; 1999. p. 222–235.

Święcicki W, Rybczyński JJ, Święcicki WK. Domestication and genetics of the yellow lupin (Lupinus luteus L.) and the biotechnological improvement of lupins. J Appl Genet. 2000;41(1):11–34.

Turner KL, Wroth JM, Cowling WA. Pro-embryos of Lupinus spp. produced from isolated microspore culture. Aust J Agric Res. 2004;55(5):589. http://dx.doi.org/10.1071/AR03226

Croser JS, Lülsdorf MM, Davies PA, Clarke HJ, Bayliss KL, Mallikarjuna N, et al. Toward doubled haploid production in the Fabaceae: progress, constraints, and opportunities. CRC Crit Rev Plant Sci. 2006;25(2):139–157. http://dx.doi.org/10.1080/07352680600563850

Skrzypek E, Czyczyło-Mysza I, Marcińska I, Wędzony M. Prospects of androgenetic induction in Lupinus spp. Plant Cell Tissue Organ Cult. 2008;94(2):131–137. http://dx.doi.org/10.1007/s11240-008-9396-7

Ochatt S, Pech C, Grewal R, Conreux C, Lulsdorf M, Jacas L. Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae). J Plant Physiol. 2009;166(12):1314–1328. http://dx.doi.org/10.1016/j.jplph.2009.01.011

Kuchel H, Langridge P, Mosionek L, Williams K, Jefferies SP. The genetic control of milling yield, dough rheology and baking quality of wheat. Theor Appl Genet. 2006;112(8):1487–1495. http://dx.doi.org/10.1007/s00122-006-0252-z

Marza F, Bai GH, Carver BF, Zhou WC. Quantitative trait loci for yield and related traits in the wheat population Ning7840 × Clark. Theor Appl Genet. 2005;112(4):688–698. http://dx.doi.org/10.1007/s00122-005-0172-3

Surma M, Adamski T, Kuczyńska A, Krystkowiak K, Trzeciak R, Mikołajczak K, et al. Zmodyfikowana technika pojedynczego ziarna w hodowli jęczmienia ozimego. Biul IHAR. 2011;262:59–66.

Ochatt SJ, Sangwan RS, Marget P, Assoumou Ndong Y, Rancillac M, Perney P. New approaches towards the shortening of generation cycles for faster breeding of protein legumes. Plant Breed. 2002;121(5):436–440. http://dx.doi.org/10.1046/j.1439-0523.2002.746803.x

Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

Zar JH. Biostatistical analysis. Englewood Cliffs NJ: Prentice-Hall; 1984.

Vuillaume E, Hoff T. Développement in vitro d’embryons immatures de Lupinus albus L. et de Lupinus mutabilis Sweet par culture de gousses, d’ovules ou d’embryons isolés. Agronomie. 1986;6(10):925–930. http://dx.doi.org/10.1051/agro:19861008

Podyma E, Turzyński D, Rybczyński JJ. An immature embryo culture, vegetative propagation and somatic cell genetic manipulation of Lupinus taxa. In: Proceedings of the 5th International Lupin Conference: July 5–8, 1988, Poznań, Poland. Warsaw: PWRiL; 1988. p. 439–443.

Kasten W, Kunert R. A culture method for isolated immature embryos of different Lupinus species. Biol Zent Bl. 1991;110:290–300.

Kasten W, Paradies T, Kunert R, Straka P. Progress in realization of interspecific hybrids in the genus Lupinus by means of an embryo rescue technique. Biol Zent Bl. 1991;110(4):301–309.

Stafford A, Davies DR. The culture of immature pea embryos. Ann Bot. 1979;44(3):315–321.

Mikołajczak K, Ogrodowicz P, Kuczyńska A, Krystkowiak K, Adamski T, Surma M. In vitro culture of immature embryos in production of single seed descent lines in pea and lupine. BioTechnologia. 2012;93(2):215.




DOI: https://doi.org/10.5586/asbp.2013.021

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