Gossypium barbadense: An Approach for in Situ Conservation in Cerrado, Brazil

Abandonment of planting of Gossypium barbadense has endangered its existence. The objective was to determine the characteristicof the maintenance of Gossypium barbadense in the Central-West Region of Brazil, with the aim to foster the conservation of the species. Expeditions were conducted in 2014-2015 in Southeast Goias, where cotton collection has not been reported before. Data from previous collections in Goias, Mato Grosso, Mato Grosso do Sul and Distrito Federal available in Albrana database were considered this study. In the Central-West Region of Brazil, 466 accesses of G. barbadense were recorded, found most frequently in backyards (91.4%), but also spontaneous plants (7.5%), farm boundary (0.8%) and commercial farming (0.2%) have also been found. The main use indicated by VDU was as medicinal plant (0.66), therefore this is the main reason for in situ preservation. However, this cultural habit has been abandoned, causing uncertainty about the continuity of preservation. There was presence of lint almost only in seeds of plants of the variety brasiliense. The most common fiber color was white, not cream color, suggesting that plants are derived from commercial cultivars used in the past. Hence, until 2015, the conservation relies on single plants almost exclusively in backyards for medicinal use, and there are no crops of this type of cotton for commercial purpose. The exploitation of the possible use both of the fiber as a medicinal plant would foster ex situ and in situ preservation of this important genetic resource.

The species G. barbadense is valued for its high fiber quality, often referred as Egyptian cotton. It had millenarian use in the manufacture of ropes, fishing nets in Africa and it has been commercialized in the United States, becoming a product of great importance in other countries like Egypt, India and Sudan (Todou & Konsala, 2011). Also known as "pima cotton" in the northern hemisphere, still has great importance for the production of high quality fabrics (Sousa, 2010). This type of cotton was widely cultivated. In Brazil, it was introduced from Peru in pre-Colombian periods, and its cultivation as economic exploitation was made from the 1760s, initially in Maranhão state and then covering a larger region of Northeast Brazil for export to England (Alves, 2006). Its cultivation has been gradually replaced by upland cotton belonging to the species G. hirsutum (Braga Sobrinho & Lukefahr, 1983), since upland cotton presents the most productive varieties and has been improved for mechanized planting, being cthe first species responsible for all the production of the world's commercial fiber (Bertini et al., 2005).
Brazil is the fifth largest cotton producer in the world (Abrapa, 2015), and the Central-West Regions the leading producer, where about 630,800 ha have been cultivated in the 2015/16 season (Conab, 2015). Immediate conservation measures are necessary to protect the species germplasm (Maxted et al., 1997), since gene flow is not controlled (Ferment & Zanoni, 2007). Research on G. barbadense cotton have highlighted its importance as a primary source of genetic variability that can be exploited to improve the commercial cotton fiber quality (Wang et al., 2011) and as a source of genetic resistance to nematode (Gutiérrez et al., 2010).
The species G. barbadense occurs in the country as cultivars and local varieties, and is not commercially cultivated in Brazil. Itcan be found in all Brazilian states most frequently as a backyard plant (Albrana, 2016). Thus it has been suggested thatin situ conservation depends on the use of the plants by the owner, which may be applied to medicinal proposes or use of the fiber (Almeida et al., 2009). The changing cultural habits, environmental depredation, agricultural expansion of commercial cotton and policies of cotton eradication with the aim of pest control have been the main threats to the disappearance of numerous local varieties of cotton, and their genetic potential has not been examined yet (Barroso et al., 2005;Menezes et al., 2010;Menezes et al. 2015).
The prospection of Southwest Goiás which had not been explored before associated to the data available at Albrana website lead to a description of G. barbadense distribution in the Central-West Region of Brazil to promote genetic resources conservation strategies.

Method and Methods
Expeditions to collect G. barbadense were conducted in the 2014-2015 period in Southeast Goiás municipalities. The expeditions started from a ginnery, which name is California, located in the city of Ipameri, GO, then extending to adjacent cities. The following points of collection were defined by adopting a methodology "snowball" (Bernard, 1994) in which a first preconceived informant indicates another possible informant with the set characteristics of knowing local cotton plants, and so on, including more participants. The expeditions were carried out with the authorization of Information and Authorization System in Biodiversity (SISBIO No. 37165), so leaves or petals were collected for genotyping studies and seeds with the aim to create a germplasm collection in IF Goiano Campus-Urutaí, GO, Brazil.
Southeast Goiás has been chosen since there were no previous cotton collections there (Albrana, 2016), while surrounding areas of Central-West Region of Brazil were represented in Embrapa collection. A total of 39 plants were collected, covering the municipalities of Bela Vista, Cristianópolis, Ipameri, Palmelo, Piracanjuba, Pires do Rio, Santa Cruz and Urutaí.
Cotton plants were scrutinized following a semi-structured form comprising the geographical coordinates (latitude, longitude and altitude obtained by Global Positioning System-GPS Garmim®, ETREX 10), information about population (number of plants per collection point, species, type of population, declared seed origin, presence of petal stain, presence of fuzz, leaf color), cultural procedures (fertilization, seed storage and processing), as well as environmental and phenological information (time of flowering, plant height and age).
The cotton type was classified according to the definition proposed by Johnston et al. (2006) wherein: i) Wild is a kind of plant that occurs only in natural environments and that has not gone through selection; ii) Feral is sexual or vegetative progeny derived from selected crop genotypes, which has survived and self propagated for a long period; iii) Backyard plants are grown in backyards or gardens and the origin of seeds is from relatives, friends or neighbors; iv) Local variety is the traditional cotton grown by farmers and seeds are those harvested from their crops; v) Volunteer or spontaneous plants are those germinated without human interference, which seeds or vegetative propagules are often from domesticated genotypes, occurring within a crop field close to it.

Permanence (Years)
Até 1 (Albrana, 2016). The conservation in backyards also occurs in Mexico (Ulloa et al., 2006). The complexity and difficulty of in situ conservation strategies lies therefore on the need for cooperation of the owners of the plants, and the possibility to foster the commitment to conservation of the species, according to the International Treaty on Plant Genetic Resources for Food and Agriculture (FAO 2016 The fuzz a can also be the most c presents cr in the 80s (Borém et

Conclus
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