Gene-set of brown planthopper genome
Dataset type: Genomic
Data released on April 20, 2015
Wang Z; Yu L; Zhou X; Zhang C (2015): Gene-set of brown planthopper genome GigaScience Database. https://doi.org/10.5524/100139
The brown planthopper, Nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. Outbreaks of it have re-occurred approximately every three years in Asia. It has also been used as a model system for ecological studies and for developing effective pest management.
The N. lugens population sequenced in the present study was originally collected in Hangzhou, China, in 2008 and had been reared on rice seedlings (strain: TN1). One male and one female were isolated and mated to produce F1 progeny. A single pair was then selected for sibling inbreeding for 13 generations. Genomic DNA was extracted from adult females of the F13 generation. We constructed a series of DNA libraries with varying insert sizes (180 bp, 200 bp, 500 bp, 2 kb, 5 kb, 10 kb, 20 kb, and 40 kb) to perform WGS sequencing. All of the above libraries were sequenced on Illumina HiSeq 2000 or GA-II sequencers. After performing filtering steps to remove adapter-contaminated reads, low-quality reads, and duplicate reads, a total of 158.01 Gbp of clean WGS data (131.67X of the genome) with 131.7-fold of genome coverage depth and were combined with 507.94 Gbp of clean fosmid sequence data was obtained. This was assembled using SOAPdenovo and Rabbit, producing a final draft assembly of 1.141Gbp with a scaffold N50 of 356,597 bp. The Nilaparvata lugens genome should highlight potential directions for effective pest control of the planthopper.
Additional details
Read the peer-reviewed publication(s):
- Xue, J., Zhou, X., Zhang, C.-X., Yu, L.-L., Fan, H.-W., Wang, Z., Xu, H.-J., Xi, Y., Zhu, Z.-R., Zhou, W.-W., Pan, P.-L., Li, B.-L., Colbourne, J. K., Noda, H., Suetsugu, Y., Kobayashi, T., Zheng, Y., Liu, S., Zhang, R., … Cheng, J.-A. (2014). Genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation. Genome Biology, 15(12). https://doi.org/10.1186/s13059-014-0521-0
Accessions (data included in GigaDB):
BioProject: PRJNA177647
Click on a table column to sort the results.
Table SettingsSample ID | Common Name | Scientific Name | Sample Attributes | Taxonomic ID | Genbank Name |
---|---|---|---|---|---|
SAMN03077662 | Nilaparvata lugens | Sex:female [PATO:0000383] Geographic location (country and/or sea,region):Hangzhou China Isolate:NLH13 |
108931 | brown planthopper |
Click on a table column to sort the results.
Table SettingsFile Name | Description | Sample ID | Data Type | File Format | Size | Release Date | File Attributes | Download |
---|---|---|---|---|---|---|---|---|
gff format file describing gene structures | Annotation | GFF | 1.85 MB | 2015-04-20 | MD5 checksum: 1735d30cd1a47533572cd6ade61b0d91 |
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protein sequences of genes | Protein sequence | FASTA | 6.46 MB | 2015-04-20 | MD5 checksum: 8b5204c3a171c2648203e3139f6b0447 |
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cds sequences of genes | Coding sequence | FASTA | 33.24 MB | 2015-04-20 | MD5 checksum: 76f300ef50230209cd6f796a1e6f80b2 |
Date | Action |
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April 27, 2015 | Dataset publish |
April 27, 2015 | Description updated from : The brown planthopper, Nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. Outbreaks of it have re-occurred approximately every three years in Asia. It has also been used as a model system for ecological studies and for developing effective pest management. The N. lugens population sequenced in the present study was originally collected in Hangzhou, China, in 2008 and had been reared on rice seedlings (strain: TN1). One male and one female were isolated and mated to produce F1 progeny. A single pair was then selected for sibling inbreeding for 13 generations. Genomic DNA was extracted from adult females of the F13 generation. We constructed a series of DNA libraries with varying insert sizes (180 bp, 200 bp, 500 bp, 2 kb, 5 kb, 10 kb, 20 kb, and 40 kb) to perform WGS sequencing. All of the above libraries were sequenced on Illumina HiSeq 2000 or GA-II sequencers. After performing filtering steps to remove adapter-contaminated reads, low-quality reads, and duplicate reads, a total of 158.01 Gbp of clean WGS data (131.67X of the genome) with 131.7-fold of genome coverage depth and were combined with 507.94 Gbp of clean fosmid sequence data was obtained. This was assembled using SOAPdenovo and Rabbit, producing a final draft assembly of 1.141Gbp with a scaffold N50 of 356,597 bp. The Nilaparvata lugens genome should highlight potential directions for effective pest control of the planthopper. |
April 27, 2015 | Dataset size updated from : 0 bytes |
October 20, 2015 | File Nlug_v1.1.fa1.Nchange.gff.gz updated |
October 20, 2015 | File Nlug_v1.1.fa1.Nchange.pep.gz updated |
October 20, 2015 | File Nlug_v1.1.fa1.Nchange.cds updated |
October 23, 2015 | File Nlug_v1.1.fa1.Nchange.cds updated |
October 23, 2015 | File Nlug_v1.1.fa1.Nchange.cds updated |
October 23, 2015 | File Nlug_v1.1.fa1.Nchange.cds updated |
October 23, 2015 | File Nlug_v1.1.fa1.Nchange.gff.gz updated |