Abstract
Screening of estrogenic activity on dichloro diphenyl trichloroethane (DDT), dichloro diphenyl dichloro ethylene (DDE), dieldrin, heptachlor, aldrin, chlordane, lindane, polybrominated diphenyl ethers (PBDE) and parabens was compared using Organization for Economic Cooperation and Development (OECD) test guideline 455 (TG455). The estrogenic activity of DDT was 58,000-fold (PC50, 1.67 × 10−6M) less than 17ß-estradiol(E2) (PC50, 2.88×10−11M) but DDE, dieldrin, heptachlor, aldrin, chlordane, lindane and PBDE did not show any estrogenic activity in this assay system. In the case of paraben compounds, the rank of relative transcriptional activation (logRTA) was butyl paraben −1.63752 (PC50, 1.25 × 10−7M) > isobutyl paraben −2.34008 (PC50, 6.3 × 10−7M) > ethyl paraben -2.64016 (PC50, 1.26 × 10−6M) > isopro-pyl paraben −2.73993 (PC50, 1.58 × 10−6M) > propyl paraben -2.84164 (PC50, 2.0 × 10−6M). Our data suggest that OECD test guideline TG455 may be useful as a screening tool for potential endocrine disruptors.
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Bonefeld-Jorgensen, E.C. and Ayotte, P. (2003) Toxicological properties of POPs and related health effects of concern for the arctic populations, in: AMAP assessment 2002: Human Health in the Arctic. AMAP., pp. 137.
Bonefeld-Jorgensen, E.C. (2004). The human health effect programme in Greenland, a review. Sci. Total Environ., 331, 215–231.
Colborn, T., vom Saal, F.S. and Soto, A.M. (1993). Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ. Health Perspect., 101, 378–384.
Coldham, N.G., Dave, M., Sivapathasundaram, S., McDonnell, D.P., Connor, C. and Sauer, M.J. (1997). Evaluation of a recombinant yeast cell estrogen screening assay. Environ. Health Per-spect., 105, 734–742.
Damdimopoulos, A.E., Spyrou, G. and Gustafsson, J.A. (2008) Ligands Differentially Modify the Nuclear Mobility of Estrogen Receptors alpha and beta. Endocrinology, 149, 339–345.
Hunter, D.S., Hodges, L.C., Vonier, P.M., Fuchs-Young, R., Gottardis, M.M. and Walker, C.L. (1999) Estrogen receptor activation via activation function 2 predicts agonism of xenoestrogens in normal and neoplastic cells of the uterine myometrium. Cancer Res., 59, 3090–3099.
Krieger, R.I. (2001). Handbook of Pesticide Toxicology, 1. Academic Press, San Diego, USA.
Nishihara, T., Nishikawa, J.I., Kanayama, T., Dakeyama, F., Saito, K., Imagawa, M., Takatori, S., Kitagawa, Y., Hori, S. and Utsumi, H. (2000) Estrogenic activities of 517 chemicals by yeast two-hybrid assay. Journal of Health Science, 46, 282–298.
OECD, Organization for Economic Cooperation and Development (2003) Draft summary report of the sixth meeting of the task force on endocrine disrupters testing and assessment (EDTA 6). Paris.
OECD, Organization for Economic Cooperation and Development (2009) Ten new test guidelines and six updated test guidelines have been adopted by council on 7 September 2009.
Okubo, T., Yokoyama, Y., Kano, K. and Kano, I. (2001) ER-dependent estrogenic activity of parabens assessed by proliferation of human breast cancer MCF-7 cells and expression of ERalpha and PR. Food Chem. Toxicol., 39, 1225–1232.
Routledge, E.J., Parker, J., Odum, J., Ashby, J. and Sumpter, J.P. (1998) Some alkyl hydroxy benzoate preservatives (Parabens) are estrogenic. Toxicol. Appl. Pharmacol., 153, 12–19.
Soni, M.G., Carabin, I.G. and Burdock, G.A. (2005) Safety assessment of esters of p-hydroxybenzoic acid (Parabens). Food Chem. Toxicol., 43, 985–1015.
Soto, A.M., Sonnenschein, C., Chung, K.L., Fernandez, M.F., Olea, N. and Srrano, F.O. (1995) The E-screen assay as a tool to identify estrogens: An update on estrogenic environmental pollutants. Environ. Health Perspect., 103, 113–122.
Vo, T.T. and Jeung, E.B. (2009) An evaluation of estrogenic activity of parabens using uterine Calbindin-D9K gene in an immature rat model. Toxicol. Sci., 11 2, 68–77.
Vos, J.G., Dybing, E., Greim, H.A. Ladefoged, O. Lambre, C. Tarazona, J.V. Brandt, I. and Vethaak, A.D. (2000) Health effects of endocrine-disrupting chemicals on wildlife, with special reference to the European situation. Crit. Rev. Toxicol., 30, 71–133.
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Kim, T.S., Kim, C.Y., Lee, H.K. et al. Estrogenic Activity of Persistent Organic Pollutants and Parabens Base on the Stably Transfected Human Estrogen Receptor-α Transcriptional Activation Assay (OECD TG 455). Toxicol Res. 27, 181–184 (2011). https://doi.org/10.5487/TR.2011.27.3.181
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DOI: https://doi.org/10.5487/TR.2011.27.3.181